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p44PPV-eGFP

Plasmid

#241907

Purpose

Part 2 of a two-plasmid PCA biosensor composed of the PcaV repressible PPV promoter upstream from a reporter gene (eGFP) on a second plasmid

Depositor

Neil Dixon

Article

F M Machado et al J Biol Eng. 2019 Nov 27;13:91. doi: 10.1

Insert

His6-eGFP

Use

Synthetic Biology

Mutation

none

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pαH-NiV-F-WT-Foldon

Plasmid

#200391

Purpose

Mammalian cell expression of Nipah virus fusion protein

Depositor

Priyamvada Acharya

Article

May et al J Virol. 2023 Jun 29;97(6):e0043323. doi

Insert

NiV-F Ectodomain

Tags

Foldon, HRV3C cleavage site, 8X His tag, 2X Strep…

Expression

Mammalian

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pαH-LayV-F-WT-Foldon

Plasmid

#200392

Purpose

Mammalian cell expression of Langya virus fusion protein

Depositor

Priyamvada Acharya

Article

May et al J Virol. 2023 Jun 29;97(6):e0043323. doi

Insert

LayV-F Ectodomain

Tags

Foldon, HRV3C cleavage site, 8X His tag, 2X Strep…

Expression

Mammalian

Mutation

Ectodomain (AA 1-483)

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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DriD-wHTH-DNABD

Plasmid

#237851

Purpose

The wHTH DNA binding domain used to understand the allosteric mechanism

Depositor

Maria Schumacher

Article

Cannistraci et al Commun Biol. 2025 Apr 29;8(1):679. doi:

Insert

DriD wHTH DNABD

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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DriD-3HB-mutant

Plasmid

#237852

Purpose

DriD 3HB surface mutant used to interrogate the allosteric mechanism

Depositor

Maria Schumacher

Article

Cannistraci et al Commun Biol. 2025 Apr 29;8(1):679. doi:

Insert

DriD 3HB mutant

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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GB4650

Plasmid

#215265

Purpose

TU for the copper regulated expression of the PhiC31 recombinase.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

CBS:miniDFR:PhiC31:tNos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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GB4652

Plasmid

#215266

Purpose

TU for the copper regulated expression of the flippase (FLP) recombinase.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

CBS:miniDFR:FLP:TNOS

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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GB1203

Plasmid

#215369

Purpose

TU for the expression of the silencing suppressor P19 driven by the 35s promoter

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

35s:P19:tNos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pCR-U6-gRNA-TRE3G-Nluc

Plasmid

#192659

Purpose

A plasmid encoding TRE3G sgRNA and TRE3G-promoter-Nluc

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Nluc

Use

Luciferase

Expression

Mammalian

Promoter

U6, TRE3G

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCR-U6-gRNA-miniCMV-Nluc

Plasmid

#192657

Purpose

A plasmid encoding miniCMV sgRNA and miniCMV-promoter-Nluc

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Nluc

Use

Luciferase

Expression

Mammalian

Promoter

U6, miniCMV

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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p415 Glo3 R59K

Plasmid

#112652

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 R59K

Expression

Yeast

Mutation

R59K (GAP-dead)

Promoter

Met25

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Gcs1 R54K

Plasmid

#112647

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Gcs1 R54K

Expression

Yeast

Mutation

R54K

Promoter

Met25

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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KA2963_eSpCas9-PGKHygdtkCh

Plasmid

#124205

Purpose

Expression of eSpCas9(1.1) and sgRNA

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Expression

Mammalian

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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KA1063_pPB-hCMV*1-cHA-IRESH2BmKO2

Plasmid

#124169

Purpose

piggyBac-based Dox-inducible expression

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Expression

Mammalian

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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KA1123_pPB-TREtight-cHA-IRESH2BmKO2

Plasmid

#124172

Purpose

piggyBac-based Dox-inducible expression

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Expression

Mammalian

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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p415 Ret2LD2a Q146F

Plasmid

#75259

Purpose

Q146F mutation

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2LD2a

Tags

His

Expression

Yeast

Mutation

Q146F

Promoter

Met25

Available Since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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p415 Ret2LD2a T158K

Plasmid

#75262

Purpose

T158K mutation

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2LD2a T158K

Tags

His

Expression

Yeast

Promoter

Met25

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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p415 Ret2LD2a K153Q

Plasmid

#75261

Purpose

K153Q mutation

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2LD2a K153Q

Tags

His

Expression

Yeast

Promoter

Met25

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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p415 Ret2LD2a N152T

Plasmid

#75260

Purpose

N152T mutation

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2LD2a N152T

Tags

His

Expression

Yeast

Promoter

Met25

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-GCN4cc-Erp2CT

Plasmid

#73923

Purpose

GST fusion- coiled coil- Erp2 cytoplasmic tail

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Erp2 CT

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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