We narrowed to 6,045 results for: pes

1. pCR3.1 GSK3beta S9A HA

   Plasmid

   #15994

   Depositor

   Morris Birnbaum

   Article

   Summers et al J Biol Chem. 1999 Jun 18. 274(25):17934-

   Insert

   GSK3beta (GSK3B Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S9A: constitutively active

   Promoter

   Available since

   Oct. 26, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pENTR_hEMSY

   Plasmid

   #125156

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

   Insert

   EMSY (EMSY Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-FDIO-dAPEX2

   Plasmid

   #117175

   Purpose

   Peroxidase reporter for multiplexed electron microscopy labeling

   Depositor

   David Ginty

   Article

   Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

   Insert

   dAPEX2
   

   Use

   AAV; Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   W41F and A134P on soybean APX

   Promoter

   EF1a

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSTAP-seq_human-4xUAS

   Plasmid

   #125150

   Purpose

   Vector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in human cells

   Depositor

   Alexander Stark

   Article

   Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

   Insert

   4 x upstream activating sequence (UAS)
   

   Use

   Stap-seq screening vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUE1-TSP-ORF68 R443A

   Plasmid

   #162652

   Purpose

   Expresses ORF68 R443A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   ORF68
   

   Use

   Tags

   N-terminal 2xStrep-Tag II and HRV 3C site

   Expression

   Mammalian

   Mutation

   Amino acid R443 mutated to alanine

   Promoter

   CMV

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-HCMVgH (Merlin)

   Plasmid

   #136448

   Purpose

   Mammalian expression plasmid for HCMV glycoprotein H (Merlin strain), full-length

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   glycoprotein H (UL75 Human betaherpesvirus 5)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMpGWB431

   Plasmid

   #68696

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Emerald Luc fused with PEST sequence [ELuc(PEST)]

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBS-KS-attB2-SA(1)-T2A-QF2-Hsp70

   Plasmid

   #62945

   Purpose

   Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 1

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-QF2-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBS-KS-attB2-SA(0)-T2A-QF2-Hsp70

   Plasmid

   #62944

   Purpose

   Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 0

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-QF2-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBS-KS-attB2-SA(2)-T2A-QF2-Hsp70

    Plasmid

    #62946

    Purpose

    Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 2

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-QF2-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBS-KS-attB2-SA(1)-T2A-LexA::GADfluw-Hsp70

    Plasmid

    #78306

    Purpose

    Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 1

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-nlsLexA::GADfluw-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSFG-TFII-I-GFP delta

    Plasmid

    #22190

    Depositor

    Ananda Roy

    Article

    Desgranges et al Mol Cell Biol. 2005 Dec . 25(24):10940-5

    Insert

    TFII-I (GTF2I Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBS-KS-attB2-SA(2)-T2A-LexA::GADfluw-Hsp70

    Plasmid

    #78307

    Purpose

    Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 2

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-nlsLexA::GADflluw
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pITGFP89

    Plasmid

    #83560

    Purpose

    Used to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    TEF1 promoter -yEGFP-CLN2PEST
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV5-Fyn C3,6S

    Plasmid

    #19979

    Depositor

    Marilyn Resh

    Article

    Alland et al J Biol Chem. 1994 Jun 17. 269(24):16701-

    Insert

    Fyn (FYN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C3,6S

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-E1.1-NRSE-RFP

    Plasmid

    #22929

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

    Insert

    E1.1 binding site from Scardigli et al. 2003, with neuron-restrictive silencing element, with minimal CMV
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMpGWB231

    Plasmid

    #68622

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Emerald Luc fused with PEST sequence [ELuc(PEST)]

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX4T2-Snf1-cat

    Plasmid

    #52683

    Purpose

    expression of the Snf1 kinase domain in bacteria

    Depositor

    Benjamin Turk

    Article

    Lee et al Mol Cell Biol. 2012 Nov;32(22):4705-17.

    Insert

    SNF1 (SNF1 Budding Yeast)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    encodes kinase domain only, aa 41-315

    Promoter

    tac

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pUE1-TSP-ORF68 K395A/K396A

    Plasmid

    #162656

    Purpose

    Expresses ORF68 K395A/K396A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Mutation

    Amino acids K395 and K396 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EGFP-bSV1_1-1009

    Plasmid

    #129471

    Purpose

    Mammalian expression of the N-terminus + central domain of bovine SV1

    Depositor

    Elizabeth Luna

    Article

    Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

    Insert

    Supervillin (SVIL Bovine)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    contains amino acids 1-1009

    Promoter

    CMV

    Available since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAGW-dpse-GAL4-DBD

    Plasmid

    #125153

    Purpose

    Gateway-compatible vector to express a fusion protein containing the GAL4-DBD (N-terminally) followed by the protein of interest under the control of a D.pseudoobscura enhancer and CP

    Depositor

    Alexander Stark

    Article

    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

    Insert

    D. pseudoobscura peak #102 enhancer and D. pseudoobscura CG13116 CP
    

    Use

    Gateway fly expression vector

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. SMT205

    Plasmid

    #134817

    Purpose

    SMT201 with mutated RBS for DHFR, in vivo assays

    Depositor

    Tanja Kortemme

    Article

    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

    Inserts

    Dihydrofolate reductase
    Thymidylate synthase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Silent mutations to eliminate internal restrictio…

    Promoter

    T7 and TET

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBS chick PEA3 [TJ#51]

    Plasmid

    #16279

    Depositor

    Thomas Jessell

    Article

    Lin et al Cell. 1998 Oct 30. 95(3):393-407.

    Insert

    PEA3 (ETV4 Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

24. pBS chick ER-81 [TJ#50]

    Plasmid

    #16274

    Depositor

    Thomas Jessell

    Article

    Lin et al Cell. 1998 Oct 30. 95(3):393-407.

    Insert

    ER81 (ETV1 Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

25. pRS426-TUB1-internal6xHis (1-440)

    Plasmid

    #60391

    Purpose

    Expression of yeast alpha tubulin (TUB1) - internal 6xHis without Cterminal tail using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB1 (TUB1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    internal 6xHis (see supplement figure 4), amino a…

    Promoter

    GAL

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pRY1004

    Plasmid

    #21788

    Depositor

    Richard Yu

    Article

    Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

    Insert

    Dig1-YFP (DIG1 A. victoria, Budding Yeast)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    contains 5' 1000bp UTR (Dig1 promoter) Di…

    Promoter

    Available since

    Sept. 18, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-Ef1a-DIO-PBG-WPRE-hGH

    Plasmid

    #74287

    Purpose

    pAAV vector to express PBG (chimeric Glycoprotein) in a Cre-dependent manner

    Depositor

    Edward Callaway

    Article

    Kim et al Neuron. 2015 Dec 16;88(6):1253-67. doi:

    Insert

    PBG (chimeric rabies Glycoprotein)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRS424-TUB2 - human TUBB2a Cterminal tail

    Plasmid

    #60396

    Purpose

    Expression of chimeric yeast beta tubulin (TUB2) - human beta2 tubulin Cterminal tail (TUBB2a) using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    TUB2 amino acids 429 - end, replaced with human T…

    Promoter

    GAL

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pTL370

    Plasmid

    #69880

    Purpose

    attB flanked genomic Cirl for integration at attP sites.

    Depositor

    Tobias Langenhan, Robert Kittel

    Article

    Scholz et al Cell Rep. 2015 May 12;11(6):866-74. doi:

    Insert

    CIRL (Cirl Fly)
    

    Use

    Phic31-integration vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pNTI751 pPGK1-GCN4(UTR)-ZEM(TF)

    Plasmid

    #164916

    Purpose

    Expresses the ZEM transcription factor under the translational control of the yeast GCN4 5'UTR

    Depositor

    Nicholas Ingolia

    Article

    Muller et al Science. 2020 Dec 11;370(6522). pii: 370

    Insert

    GCN4(UTR)-ZEM(TF)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK1 (yeast)

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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