Addgene

We narrowed to 18,120 results for: Met;

Showing: 2601 - 2640 of 18120 results

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pGTag Hygro EGFP nLAP +2

Plasmid

#194307

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

Insert

Hygro-P2A-nLAP(EGFP)

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_Uricase

Plasmid

#182400

Purpose

Bacterial expression of uricase

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

uricase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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ubc-nls-pcp-10xSunTag (SRv1.1)

Plasmid

#185798

Purpose

pcp-10xSunTag component for SunRISER SRv1.1

Depositor

Robin Lee

Article

Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

Insert

pcp-10xSunTag

Use

Tags

FactorXa site, HA, and NLS

Expression

Mammalian

Mutation

Promoter

human ubiquitin C promoter

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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ubc-nls-pcp-12xSunTag (SRv1.2)

Plasmid

#185799

Purpose

pcp-12xSunTag component for SunRISER SRv1.2

Depositor

Robin Lee

Article

Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

Insert

pcp-12xSunTag

Use

Tags

FactorXa site, HA, and NLS

Expression

Mammalian

Mutation

Promoter

human ubiquitin C promoter

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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His-TEV-GB1 Initial Gly

Plasmid

#128685

Purpose

Expresses GB1 mutant with no Methionine residues

Depositor

Sharon Rozovsky

Article

Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

Insert

spg

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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CSM160

Plasmid

#105684

Purpose

Plasmid containing an RFP dropout used to construct randomized PAM library

Depositor

Chase Beisel

Article

Maxwell et al Methods. 2018 Jul 1;143:48-57. doi: 10.1

Insert

RFP Golden Gate dropout

Use

Tags

Expression

Bacterial

Mutation

Promoter

Bba_R0040 TetR Promoter

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pPD027 Reporter Template v1 (Near Spaced)

Plasmid

#138751

Purpose

Template for COMET promoters, expresses EYFP reporter upon promoter activation.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pPD152 Reporter Template v2

Plasmid

#138752

Purpose

Template for COMET promoters, expresses EYFP reporter upon promoter activation. BsaI sites produce palindromic sticky ends.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPD032 Reporter Template v1 (Mid Spaced)

Plasmid

#138753

Purpose

Template for COMET promoters, expresses EYFP reporter upon promoter activation.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPD033 Reporter Template v1 (Far Spaced)

Plasmid

#138754

Purpose

Template for COMET promoters, expresses EYFP reporter upon promoter activation.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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EMX1*_sgRNA

Plasmid

#100558

Purpose

Expresses EMX1* sgRNA. Target sequence: (G)GAGTCCGAGCAGAAGAAGAA. Same target sequence as #100555, except with an additional 5' G.

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

EMX1 sgRNA

Use

Tags

Expression

Mutation

Promoter

U6

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pAP1071-2

Plasmid

#99502

Purpose

3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3utr::linker::TEV

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3’utr::linker::TEV

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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TF101-STTM-miR390

Plasmid

#84812

Purpose

Turning off/down miR390 in Soybean

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM-miR390

Use

Tags

Expression

Plant

Mutation

Promoter

unknown

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-194-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85929

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-194-3p Sensor

Use

Lentiviral

Tags

Expression

Mutation

Promoter

PGK

Available since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-526a.sensor.PGK.dNGFR.WPRE

Plasmid

#85900

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-526a Sensor

Use

Lentiviral

Tags

Expression

Mutation

Promoter

PGK

Available since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-486-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85883

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-486-3p Sensor

Use

Lentiviral

Tags

Expression

Mutation

Promoter

PGK

Available since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-301b.sensor.PGK.dNGFR.WPRE

Plasmid

#85850

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

mir-301b Sensor

Use

Lentiviral

Tags

-

Expression

Mutation

Promoter

PGK

Available since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-181a-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85871

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-181a-3p Sensor

Use

Lentiviral

Tags

Expression

Mutation

Promoter

PGK

Available since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AB.pCCL.sin.cPPT.GFP.miR-425-5p.sensor.PGK.dNGFR.WPRE

Plasmid

#85858

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-425-5p Sensor

Use

Lentiviral

Tags

Expression

Mutation

Promoter

PGK

Available since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AB.pCCL.sin.cPPT.GFP.miR-512-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85879

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-512-3p Sensor

Use

Lentiviral

Tags

Expression

Mutation

Promoter

PGK

Available since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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TF101-STTM-miR172

Plasmid

#84815

Purpose

Turning off/down miR172 in Soybean

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM-miR172

Use

Tags

Expression

Plant

Mutation

Promoter

unknown

Available since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only
TF101-STTM-miR408

Plasmid

#84814

Purpose

Turning off/down miR408 in Soybean

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM-miR408

Use

Tags

Expression

Plant

Mutation

Promoter

unknown

Available since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only
MSCV-6NBC-GFP-PGK-Puro-Barcode49

Plasmid

#83726

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode49

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode42

Plasmid

#83719

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode42

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode18

Plasmid

#83695

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode18

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode92

Plasmid

#83769

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode92

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode90

Plasmid

#83767

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode90

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode28

Plasmid

#83705

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode28

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode15

Plasmid

#83692

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode15

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ1852-5 pHR: U6-SpsgCD95-3 CMV-EGFP

Plasmid

#84253

Purpose

Expresses Sp sgCD95-3 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgCD95-3

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ1852-3 pHR: U6-SpsgCD95-2 CMV-EGFP

Plasmid

#84252

Purpose

Expresses Sp sgCD95-2 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgCD95-2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode94

Plasmid

#83771

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode94

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode95

Plasmid

#83772

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode95

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode96

Plasmid

#83773

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode96

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode80

Plasmid

#83757

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode80

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode82

Plasmid

#83759

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode82

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode84

Plasmid

#83761

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode84

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode85

Plasmid

#83762

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode85

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode86

Plasmid

#83763

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode86

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode87

Plasmid

#83764

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode87

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 2601 - 2640 of 18120 results

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