We narrowed to 8,219 results for: Lif
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Plasmid#207615PurposeZebrafish Alk8 BMP receptor kinase domain fused to VfLOVDepositorInsertAlk8 (acvr1l) kinase domain + VfLOV domain (acvr1l Zebrafish, Vaucheria frigida)
Available SinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA-sfGFP
Plasmid#162689PurposepFA vector control expressing sfGFPDepositorInsertsfGFP
TagsC terminal 6x His tagExpressionBacterialPromoterPL tet promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME-PLIN2-tdTOMATO
Plasmid#172533PurposePLIN2-tdTOMATO fusion (zebrafish lipid droplet reporter) in middle entry vector for Gateway cloningDepositorInsertplin2-tdtomato
MutationS80R, A81S, E144D, I148F, and R415H mutations in …Available SinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mcherry-WIPI2d-cs-TEV-STREP
Plasmid#178912PurposeMammalian expression of human WIPI2d with N-terminal mCherry and C-terminal StrepDepositorInsertWIPI2d
TagsC term strep and N term mcherryExpressionMammalianAvailable SinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Halo-53BP1 HRD
Plasmid#207087PurposeHomologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous 53BP1 locus.DepositorInsertHaloTag with internal PuroR cassette flanked by human 53BP1 locus sequences
TagsHaloTagExpressionMammalianPromoterEndogenousAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mCherry_SNAP_SNAPV_PACT_Active
Plasmid#173072PurposeActive SNAP tag at the centrosomeDepositorInsertmCherry-SNAP-SNAP-Varied-PACT
TagsmCherry-SNAP-SNAP-VariedExpressionMammalianPromoterCMV IE94Available SinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPYtag-Histag-Avidin
Plasmid#214836PurposeThis plasmid encodes a protein (SPYtag-Histag-Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertSPYtag-Histag-Avidin
ExpressionBacterialPromoterT7Available SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cys-60nmSAH-SPYcatcher
Plasmid#214840PurposeThis plasmid encodes a protein (Cys-60nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-60nmSAH-SPYcatcher
TagsHis-TEV-SUMO-ExpressionBacterialPromoterT5Available SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
ER-mCherry-eMagA
Plasmid#162248PurposeBlue-light dependent heterodimerization systemDepositorInsertCytP450 ER targeting sequencel-mCherry-eMagA
ExpressionMammalianPromoterbeta-actin promoterAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
P307-FBP_6
Plasmid#139697PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, control pathwayDepositorInsertpLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
UseLuciferaseExpressionPlantPromotervariousAvailable SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
Non tagged Avidin
Plasmid#214835PurposeThis plasmid encodes a protein (Non tagged Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertNon tagged Avidin
ExpressionBacterialPromoterT7Available SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-11b_PcrA_M6_H93A
Plasmid#194156PurposeEngineered PcrA_M6 helicase to support isothermal amplification method SHARPDepositorInsertEngineered helicase PcrA_M6
Tags6x His tag and FLAG tagExpressionBacterialMutationH93A, C96A, N187C, C247A, L384V, L409CAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai
Plasmid#202569PurposeExpresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the R261A mutation and a GFP retrotransposition reporter (GFP-AI) in the 3' UTRDepositorInsertLINE-1
TagsGFP-AI retrotransposition reporter and HaloTag7 o…ExpressionMammalianMutationChanged ORF1p Arginine 261 to AlanineAvailable SinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
OSCA3.1
Plasmid#136596PurposeExpresses the mechanosensitive channel OSCA3.1 in mammalian cellsDepositorAvailable SinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-cuOx144_Bxb1attB_loxP-PGKpuro-loxP
Plasmid#119903PurposeBxb1 integration plasmid for cuO array with Puro selection cassetteDepositorInsertcuOx144 array
UseMouse TargetingAvailable SinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
OSCA4.1
Plasmid#136597PurposeExpresses the mechanosensitive channel OSCA4.1 in mammalian cellsDepositorAvailable SinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
SAC1deltaTMD-FKBP-mCherry
Plasmid#108123PurposeRecruitable cytosolic SAC1DepositorInsertSACM1L(1-520):FKBP1A(3-108):mCherry (SACM1L Human)
ExpressionMammalianMutationSACM1L(1-520)PromoterCMVAvailable SinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-GFP
Plasmid#86537PurposeRetrovirus expressing GFPDepositorInsertGFP
UseRetroviralExpressionMammalianPromoterSynapsin I (human)Available SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AAV2cap
Plasmid#198014PurposeFor high-level expression of the AAV2 cap gene, suitable for use with pCMV-Rep78/68 SSM libraryDepositorInsertAAV2 cap
UseAAVExpressionMammalianMutationWTPromoterCMVAvailable SinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR iRFP-ZtSH2
Plasmid#188627PurposeFluorescent reporter for CD3ε/ZtSH2 pYtag biosensorDepositorInsertZtSH2
UseLentiviralTagsiRFPExpressionMammalianPromoterSFFVAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only