We narrowed to 1,501 results for: Mbp

1. UR1Ct (265-1040)

   Plasmid

   #115647

   Purpose

   expression of truncated Rag1 including the RING, E3 ubiquitin ligase (265-1008) and C-terminal region (384-1040)

   Depositor

   Martin Gellert

   Article

   Grundy et al Proc Natl Acad Sci U S A. 2010 Dec 28;10

   Insert

   Rag1 (265-1040) (Rag1 Mouse)
   

   Use

   Tags

   8XHis-MBP-PreScission

   Expression

   Mammalian

   Mutation

   truncated Rag1 (aa 265-1040)

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. SLIRP.HMG6.wt.fl

   Plasmid

   #49821

   Purpose

   expression vector for MBP-SLIRP fusion

   Depositor

   Thomas Cech

   Article

   McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

   Insert

   SLIRP SRA stem-loop interacting RNA binding protein (SLIRP Human)
   

   Use

   Tags

   E. coli Maltose Binding Protein

   Expression

   Bacterial

   Mutation

   wildtype codon-optimized for E. coli expression

   Promoter

   T7

   Available since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-FLAG-eSpCas9

   Plasmid

   #126769

   Purpose

   Expression of increased fidelity eSpCas9 in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   eSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A

   Promoter

   T7

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAB043a

   Plasmid

   #79222

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ lambda cI-MBP; PlacIq lacI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PlacZ; PlacIq

   Available since

   April 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJCC_164 SpCas9 R1333A/R1335A

   Plasmid

   #179528

   Purpose

   For bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

   Insert

   SpCas9 R1333A/R1335A
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   changed arginines 1333 and 1335 to alanine

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJCC_103 SpCas9 T1337C

   Plasmid

   #179525

   Purpose

   For bacterial expression of SpCas9 T1337C (for DNA cross-linking) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

   Insert

   SpCas9 T1337C
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   changed threonine 1337 to cysteine

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. bdSUMO-5.3-GFP-HiBit

   Plasmid

   #171014

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with 5.3 peptide and NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-5.3-muGFP-HiBiT
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. EcNusGKOW

   Plasmid

   #113124

   Purpose

   Expresses C-terminus of E. coli NusG

   Depositor

   James Berger

   Article

   Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

   Insert

   NusG (nusG Escherichia coli)
   

   Use

   Tags

   His6-MBP-N10-TEV

   Expression

   Bacterial

   Mutation

   Deleted residues 1-124

   Promoter

   T7

   Available since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. EcRfaHKOW

   Plasmid

   #113125

   Purpose

   Expresses C-terminus of E. coli RfaH

   Depositor

   James Berger

   Article

   Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

   Insert

   RfaH (rfaH Escherichia coli)
   

   Use

   Tags

   His6-MBP-N10-TEV

   Expression

   Bacterial

   Mutation

   Deleted residues 1-107

   Promoter

   T7

   Available since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJB03

    Plasmid

    #111961

    Purpose

    Expresses MBP-GFP-DHFR; construct generated for pull-down and localization experiments

    Depositor

    Matthew Good

    Article

    Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

    Inserts

    mEGFP
    eDHFR
    

    Use

    Tags

    DHFR

    Expression

    Bacterial

    Mutation

    eDHFR;residues 2-159 and enhanced GFP with monome…

    Promoter

    Tac

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. ICMAB - pET22b T7 EncFtn Mms6

    Plasmid

    #225538

    Purpose

    Construction of intracellular magnetite accumulating bacterial systems.

    Depositor

    Urartu Seker

    Article

    Yavuz et al Small. 2022 Jul;18(26):e2200537. doi: 10

    Inserts

    Iron binding protein
    Ferroxidase
    Material binding peptite
    Iron transporter protein
    

    Use

    Tags

    no

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMTTH+H1.4

    Plasmid

    #157796

    Purpose

    Expression of human linker histone H1.4

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    H1.4 (H1-4 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTAC

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJ4M_hnRNPA2_FL_WT

    Plasmid

    #139109

    Purpose

    Express FL hnRNPA2 with a C-terminal MBP tag

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant

    Plasmid

    #101209

    Purpose

    Bacterial expression plasmid for SpCas9-HF1 variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant N497A/R661A/Q695A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A and Q926A

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJSC114 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant

    Plasmid

    #101215

    Purpose

    Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant K848A/K1003A/R1060A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    K848A, K1003A and R1060A

    Promoter

    T7

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. hCD12

    Plasmid

    #225541

    Purpose

    Expresses human histone deacetylase 6 (HDAC6) catalytic domain 1 and 2 (CD12), also known as hCD12, in Escherichia coli cells.

    Depositor

    David Christianson

    Article

    Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi

    Insert

    Human Histone Deacetylase 6 Catalytic Domain 1 and 2 (HDAC6 Human)
    

    Use

    Tags

    His6-MBP tag with cleavable TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJ4M_hnRNPA2_FL_D290V

    Plasmid

    #139110

    Purpose

    Express FL hnRNPA2 D290V with a C-terminal MBP tag

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D290V

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. B1 Integrin-GFP

    Plasmid

    #186138

    Purpose

    For expression of recombinant beta1 integrin cytosolic domain with C-terminal GFP tag in bacteria

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    ITGB1 (ITGB1 Human)
    

    Use

    Tags

    MBP and mEGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSHS325 - Bacterial expression plasmid for SpCas9 REC3 domain

    Plasmid

    #101205

    Purpose

    Bacterial expression plasmid for SpCas9 REC3 domain

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant K506–Q712
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    K506–Q712

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJ4M_hnRNPA2_FL_P298L

    Plasmid

    #139111

    Purpose

    Express FL hnRNPA2 P298L with a C-terminal MBP tag

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    P298L

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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