We narrowed to 5,945 results for: pcas

1. pTrex-b-NLS-hSpCas9

   Plasmid

   #62543

   Purpose

   Expression of Cas9 in T. cruzi

   Depositor

   Rick Tarleton

   Article

   Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.

   Insert

   NLS-hSpCas9
   

   Use

   CRISPR; Trypanosoma cruzi expression

   Tags

   3xFLAG and NLS

   Promoter

   Ribo-HX1

   Available Since

   April 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. G1333 DddAtox-N–dSpCas9

   Plasmid

   #157833

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   bpNLS–G1333 DddAtox-N–dSpCas9–UGI–UGI–bpNLS
   

   Expression

   Mammalian

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-cofilin S3A

   Plasmid

   #163604

   Purpose

   Expresses constitutively active cofilin in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   cofilin1 (Cfl1 Mouse)
   

   Expression

   Mammalian

   Mutation

   S3A substitution to make cofilin constitutively a…

   Available Since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMA-SpCas9-g10

   Plasmid

   #80793

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 10/20/30 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG1.1-TSSK2/3xFLAG

   Plasmid

   #199625

   Purpose

   Expression vector of mouse TSSK2. 3xFLAG tag at C-terminus.

   Depositor

   Masahito Ikawa

   Article

   Shimada et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

   Insert

   Tssk2 mRNA (Tssk2 Mouse)
   

   Tags

   3xFLAG

   Promoter

   CAG

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-Ace2_D92N_E199V-mNeon-ST

   Plasmid

   #129261

   Purpose

   Express soma-localized Ace2_D92N_E199V-mNeon in mammalian cells

   Depositor

   Eric Schreiter

   Article

   Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

   Insert

   Ace2_D92N_E199V-mNeon-ST
   

   Expression

   Mammalian

   Mutation

   D92N, E199V

   Promoter

   CAG

   Available Since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCaST-elav-VP16AD

   Plasmid

   #15308

   Depositor

   Benjamin White

   Article

   Luan et al Neuron. 2006 Nov 9. 52(3):425-36.

   Insert

   VP16AD-ZIP+
   

   Expression

   Insect

   Available Since

   July 6, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-Venus-MDGA2

   Plasmid

   #82544

   Purpose

   Overexpression vector for Venus-MDGA2 in neurons

   Depositor

   Alice Ting

   Article

   Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

   Insert

   MDGA2 (Mdga2 Rat)
   

   Tags

   Venus fluorescent protein

   Expression

   Mammalian

   Promoter

   chicken beta actin

   Available Since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-NL-DHFR-SpCas9

   Plasmid

   #124522

   Purpose

   Expresses SpCas9 fused to DHFR domains on both the N-terminus and an internal loop in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

   Insert

   NL-DHFR-SpCas9
   

   Use

   AAV

   Tags

   Destabilized domain of E.coli dihydrofolate reduc…

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-mDrc7-FLAG

    Plasmid

    #176470

    Purpose

    Expression vector of mouse dynein regulatory complex subunit 7 (Drc7) tagged with FLAG at C-terminus.

    Depositor

    Masahito Ikawa

    Article

    Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

    Insert

    dynein regulatory complex subunit 7 (Drc7 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330_Actb sgRNA / hSpCas9

    Plasmid

    #172832

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1of Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 1 of Actb under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-ChETA-mRuby2-ST

    Plasmid

    #109126

    Purpose

    Cation channelrhodopsin ChETA fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    ChETA-mRuby2-ST
    

    Expression

    Mammalian

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9(BB)-2A-GFP-G3BP2(1)

    Plasmid

    #136060

    Purpose

    G3BP2 gRNA (#1) inserted into the pSpCas9(BB)-2A-GFP plasmid (TCATACTAAAATTCGTCATG)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP2 (G3BP2 Human)
    

    Expression

    Mammalian

    Available Since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-nDsRedFL-miR9/9*

    Plasmid

    #177805

    Purpose

    The pre-miR-9/9* sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

    Depositor

    Rikako Sanuki

    Article

    Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

    Insert

    miR-9/9*
    

    Expression

    Mammalian

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL3-B-161;+80pCalb2

    Plasmid

    #66745

    Purpose

    luciferase reporter for Calb2 promoter (-161to +80, WT)

    Depositor

    Rolf Stahel, Emanuela Felley-Bosco

    Article

    Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

    Insert

    Calb2 promoter (-161bp+80) (CALB2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    Calb2

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSpCas9n(BB)-2A-puro-a5 guide B

    Plasmid

    #194011

    Purpose

    For CRISPR knockout of integrin alpha5

    Depositor

    Timothy Springer

    Article

    Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

    Insert

    single-guide RNA (a5_guide B)
    

    Use

    CRISPR

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSpCas9n(BB)-2A-puro-aV guide A

    Plasmid

    #194008

    Purpose

    For CRISPR knockout of integrin alphaV

    Depositor

    Timothy Springer

    Article

    Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

    Insert

    single-guide RNA (aV_guide A)
    

    Use

    CRISPR

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSpCas9n(BB)-2A-puro-aV guide B

    Plasmid

    #194009

    Purpose

    For CRISPR knockout of integrin alphaV

    Depositor

    Timothy Springer

    Article

    Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

    Insert

    single-guide RNA (aV_guide B)
    

    Use

    CRISPR

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSpCas9n(BB)-2A-puro-a5 guide A

    Plasmid

    #194010

    Purpose

    For CRISPR knockout of integrin alpha5

    Depositor

    Timothy Springer

    Article

    Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

    Insert

    single-guide RNA (a5_guide A)
    

    Use

    CRISPR

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-Ace2_D92N_E199V-mRuby3-ST

    Plasmid

    #129262

    Purpose

    Express soma-localized Ace2_D92N_E199V-mRuby3 in mammalian cells

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    Ace2_D92N_E199V-mRuby3-ST
    

    Expression

    Mammalian

    Mutation

    D92N, E199V

    Promoter

    CAG

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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