175,581 results
-
Plasmid#174828PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)DepositorInsertPEmax-P2A-hMLH1dn
TagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationDetailed in manuscript; deletion of residues 754-…PromoterCMVAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-GFP (PX458)
Plasmid#48138PurposeCas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNADepositorHas ServiceCloning Grade DNAInserthSpCas9
UseCRISPRTags3XFLAG and GFPExpressionMammalianPromoterCbhAvailable SinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
mini-pHelper-1.0
Plasmid#230933PurposeThe mini version (8.4 kb) of Ad helper plasmid to provide E2A, E4orf6, and VA RNA for AAV productionDepositorTypeEmpty backboneUseAAVAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro (PX459) V2.0
Plasmid#62988PurposeCas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)DepositorInserthSpCas9-2A-Puro V2.0
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianPromoterCbhAvailable SinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-mCherry (AAV Retrograde)
Viral Prep#50459-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn-DIO-mCherry (#50459). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-mCherry plasmid DNA. hSyn-driven, Cre-dependent mCherry-expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherry (Cre-dependent)Available SinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 puro
Plasmid#8453Purpose3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses puromycin for selection.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAiExpressionMammalianAvailable SinceJuly 18, 2005AvailabilityAcademic Institutions and Nonprofits only -
pTargetF
Plasmid#62226PurposeConstitutive expression of sgRNA without donor editing template DNADepositorInsertsgRNA
UseCRISPRExpressionBacterialPromoterpij23119Available SinceMarch 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-GFP (AAV Retrograde)
Viral Prep#37825-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. GFP expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsGFPAvailable SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCF159_BRL
Plasmid#225962PurposeCMV-Intron-BRL (env protein). Expresses BRL (BaEVRLess) env for VLP production.DepositorInsertCMV-Intron-BRL (env protein)
ExpressionMammalianAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
CRISPRi GuEST-List GFP v1
Pooled Library#248189PurposeThe CRISPRi Guest for Enrichment-based Screening of Targets Library (CRISPRi GuEST-List v1) enables genome-wide, single-cell pooled screens conducted with the Flex workflow from 10x Genomics.DepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable SinceFeb. 17, 2026AvailabilityAcademic Institutions and Nonprofits only