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Plasmid#21921DepositorInsertTruncated human HKII cDNA (lacking the sequence encoded by exon 1, which is the mitochondrial binding domain) in pGFP-N3 plasmid (HK2 Human)
UseTagsGFPExpressionMammalianMutationThis is the truncated human HKII cDNA sequence (l…PromoterAvailable sinceOct. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
CMV-GEX-GECO1
Plasmid#32443PurposeMammalian expression of green excitation ratiometric genetically encoded Ca2+ indicator for optical imagingDepositorInsertGEX-GECO1.0
UseTagsExpressionMammalianMutationGCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…PromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
-227 hTF (mutant AP-1)
Plasmid#15444DepositorInsertTissue factor promoter (F3 Human)
UseLuciferaseTagsExpressionMammalianMutationBoth AP-1 sites mutatedPromoterAvailable sinceJuly 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET29a-hNABP2
Plasmid#128307PurposeExpress hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tagDepositorInsertHuman Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)
UseTagsHisExpressionBacterialMutationY85C- please see depositor comments belowPromoterT7Available sinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV-mouse p19ARF-IRES-GFP
Plasmid#107147PurposeMammalian expression of mouse ArfDepositorInsertArf [alternative reading frame] (Cdkn2a Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3-OF (mutant TCF-4 sites)
Plasmid#16559DepositorInsertTCF-4 binding sites (TCF4 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutation3 copies of mutant Tcf-4 binding sitesPromoterAvailable sinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pRSET mNG-GECO1
Plasmid#201933PurposeExpresses mNG-GECO1 indicator in bacteriaDepositorInsertmNeonGreenGECO1
UseTagsHIS tagExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCI-MS2V5-PABPC1 M161A/D165K
Plasmid#65809Purposeexpression of PABPC1-MS2 fusion. Mutations abolish binding to eIF4GDepositorInsertPABPC1 (PABPC3 Human)
UseTagsMS2 stem loops and V5ExpressionMammalianMutationM161A/D165KPromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR SFFVp MCP-mCherry IRES h2b-diRFP
Plasmid#102351PurposeLentiral expression vector bearing the MS2 Coat Protein (MCP) fused to mCherry as well as the nuclear marker histone 2b fused to two copies of irFP. Also bears the hygromycin selection marker.DepositorInsertMS2 Coat Protein
UseLentiviralTagsmCherryExpressionMutationPromoterSFFVAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (AA 366-852)
Plasmid#119315Purposelentiviral vector for expression of HA-Tagged E6-AP I AA 366-852 in mammalian cellsDepositorInsertE6-AP I (UBE3A Human)
UseLentiviralTagsHAExpressionMutationOnly AA 366-852PromoterCMVAvailable sinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
lenti-EGFR-T790M-tmpknot-epegRNA
Plasmid#214093PurposeLentiviral vector expressing epegRNA to induce EGFR T790M mutationDepositorInsertEGFR T790M epegRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET26b-SET
Plasmid#8822DepositorInsertSET (SET Human)
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
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AMPK-β2-MYC
Plasmid#40606DepositorUseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
p13xQUAS-MCS, α-cry:mCherry
Plasmid#180481PurposeInducible gene expression vector p13xQUAS-MCS, α-cry:mCherryDepositorInsertMultiple cloning site
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET23 P53DBD
Plasmid#174503Purposeexpress P53 DNA binding domain (94-312) in E.coli without tagDepositorInsertP53 DNA binding domain (corresponds to amino acid 94-312) (TP53 Human)
UseTagsnoneExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
lenti-GBA-N370S-tmpknot-epegRNA
Plasmid#214091PurposeLentiviral vector expressing epegRNA to induce GBA N370S mutationDepositorInsertGBA N370S epegRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pBV-luc TOP2A 1kb promoter
Plasmid#138274PurposeLuciferase reporter plasmid driven by TOP2A promoterDepositorInsertTOP2A promoter sequence
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only