We narrowed to 7,818 results for: Lif

1. pWaldo-GFPe-SH1446

   Plasmid

   #112507

   Purpose

   Expresses SH1446 in E. coli with a C-terminal TEV-GFP-His(8) tag.

   Depositor

   Simon Newstead

   Article

   Minhas et al Elife. 2018 Jul 3;7. pii: 34995. doi: 10

   Insert

   POT family transporter
   

   Use

   Tags

   C terminal TEV GFP octa-His affinity tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJCC_039 FnCas12a

   Plasmid

   #171666

   Purpose

   For bacterial expression of FnCas12a with a C-terminal sorting motif and His tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   FnCas12a
   

   Use

   Tags

   6X His and sortase A sorting motif

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. RNF168-Halo HRD

   Plasmid

   #207080

   Purpose

   Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF168 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   HaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   None

   Available since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME-PLIN2-tdTOMATO

   Plasmid

   #172533

   Purpose

   PLIN2-tdTOMATO fusion (zebrafish lipid droplet reporter) in middle entry vector for Gateway cloning

   Depositor

   Richard White

   Article

   Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

   Insert

   plin2-tdtomato
   

   Use

   Tags

   Expression

   Mutation

   S80R, A81S, E144D, I148F, and R415H mutations in …

   Promoter

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMS84 (U6p::GGACAGTCCTGCCGAGGTGG)

   Plasmid

   #193852

   Purpose

   Encodes the guide RNA targeting the sequence GGACAGTCCTGCCGAGGTGG

   Depositor

   Patrick Phillips

   Article

   Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

   Insert

   Guide RNA
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF HaloTag-SYB2

   Plasmid

   #197282

   Purpose

   Encodes full-length Synaptobrevin 2 (SYB2) with mutations Q76V and F77W to confer resistance to tetanus cleavage.

   Depositor

   Edwin Chapman

   Article

   Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

   Insert

   SYB2 and HaloTag (Vamp2 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q76V and F77W

   Promoter

   EF1alpha

   Available since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR R4 ATCC LacZ GCTT

   Plasmid

   #173668

   Purpose

   Destination vector for Golden Gate Cloning assemblies, blue-white screening

   Depositor

   Eric Marois

   Article

   Green et al Elife. 2023 Dec 5;12:e93142. doi: 10.755

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. P305-FBP_4

   Plasmid

   #139695

   Purpose

   T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

   Depositor

   Daniel Voytas

   Article

   Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

   Insert

   pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:tAtuOCS, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
   

   Use

   Luciferase

   Tags

   Expression

   Plant

   Mutation

   Promoter

   various

   Available since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST_6xUAS:miRFP670-cetn4

   Plasmid

   #192357

   Purpose

   zfCETN4 tagged with miRFP670, expressed under the UAS promoter

   Depositor

   Darren Gilmour

   Article

   Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

   Insert

   CETN4 (cetn4 Zebrafish)
   

   Use

   Tags

   miRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-FAK W266A

    Plasmid

    #186149

    Purpose

    For expressing FAK in mammalian cells

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PTK2 (PTK2 Mouse)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    July 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDAS12124_PEAR-GFP-preedited

    Plasmid

    #177179

    Purpose

    PEAR-GFP control plasmid with split EGFP and intact 5' splice site

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    EGFP split with an intron between amino acids 95-96
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. U6c

    Plasmid

    #117223

    Purpose

    Expresses gRNA in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

    Insert

    HR U6c promoter-gRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6

    Available since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBMN-dGBP1-TagBFP

    Plasmid

    #80087

    Purpose

    MMLV retroviral vector for expression of dGBP1-TagBFP with t-hcRed as an infection/transfection marker

    Depositor

    Connie Cepko

    Article

    Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

    Insert

    dGBP1-TagBFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. U6b

    Plasmid

    #117222

    Purpose

    Expresses gRNA in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

    Insert

    HR U6b promoter-gRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6

    Available since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLIC-lyn11-myc-LOV2GIVe

    Plasmid

    #170623

    Purpose

    For the mammalian expression of mLOV2GIVe

    Depositor

    Mikel Garcia-Marcos

    Article

    Garcia-Marcos et al Elife. 2020 Sep 16;9. pii: 60155. doi: 1

    Insert

    Lyn11-myc-LOV2GIVe
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. OSCA3.1

    Plasmid

    #136596

    Purpose

    Expresses the mechanosensitive channel OSCA3.1 in mammalian cells

    Depositor

    Ardem Patapoutian

    Article

    Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

    Insert

    OSCA3.1 (ERD4 Mustard Weed)
    

    Use

    Tags

    pIRES2-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. MMLV-CAG-G_oT7pol-IRES-BSD

    Plasmid

    #172369

    Purpose

    Generation of oT7pol-expressing stable cell lines, resistant to the antibiotic Blasticidin

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    oT7pol + BSD
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SAC1deltaTMD-FKBP-mCherry

    Plasmid

    #108123

    Purpose

    Recruitable cytosolic SAC1

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    SACM1L(1-520):FKBP1A(3-108):mCherry (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SACM1L(1-520)

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. MMLV-CAG-SADB19_oG-IRES-Puro

    Plasmid

    #172367

    Purpose

    Generation of stable cell lines expressing the rabies optimized glycoprotein (oG), resistant to the antibiotic Puromycin

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    oG + Puro
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV-hSyn-jGCaMP7b-XC

    Plasmid

    #182221

    Purpose

    Improved genetic encoded calcium sensor based on GCaMP7b.

    Depositor

    Xiaodong Liu

    Article

    Geng et al eLife. 2022 Oct 5;11:e76691. doi: 10.755

    Insert

    jGCaMP7b-XC
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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