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Plasmid#186573PurposeExpression of "dark MitoTrap" - an FRB-domain targeted to the mitochondria that is the same size as pMito-mCherry-FRB but is non-fluorescent - in human cellsDepositorInsertDark MitoTrap
UseTagsExpressionMammalianMutationMutation of "K70N" to render mCherry no…PromoterCMVAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TK-Firefly-cjBCCIP
Plasmid#122278PurposeLentiviral vector for stable expression of Firefly luciferase.DepositorInsertsFirefly luciferase
cjBCCIP 3'UTR
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV syn CCR6-eLOV-TEVcs-tTA
Plasmid#173116PurposeExpresses CCR6 receiver construct in neuronsDepositorInsertCCR6-eLOV-TEVcs-tTA
UseAAVTagsExpressionMutationPromoterSynapsinAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
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LentiCRISPRv2puro-Tyr
Plasmid#170293PurposeA knock-out vector for the mouse tyrosinase.DepositorInsertA gRNA targeting the mouse tyrosinase gene.
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nephrin
Plasmid#186134PurposeFor expression of recombinant nephrin in bacteria cellsDepositorInsertNPHS1 (NPHS1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationAmino acid residues 1174-1223, with mutations: Y1…PromoterLacAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRecLV103-GFP-PGBD5
Plasmid#65409PurposeLentiviral expression vector for fusion of GFP with human PGBD5DepositorInsertPGBD5 (PGBD5 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pUE1-TSP-ORF68
Plasmid#162650PurposeExpresses ORF68 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage siteDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag II and HRV 3C siteExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAG98-mTurquoise2-Nuf2
Plasmid#80760PurposeExpress Nuf2 N-terminally labeled with mTurquoise2DepositorInsertNuf2 (NUF2 Human)
UseRetroviralTagsmTurquoise2ExpressionMammalianMutationPromoterAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX208-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
Plasmid#125231PurposeFor generating SPARK2 NanoLuc-βarrestin2-TEVp lentivirusDepositorInsertNanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
CALNL-RARa-VP16
Plasmid#185560PurposeCre Dependent ubiquitous expression of constitutively active Retinoic Acid Receptor alphaDepositorInsertRARa-VP16 (Rara Mouse)
UseTagsVP16ExpressionMammalianMutationPromoterCAGAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Opto-Alk8
Plasmid#207615PurposeZebrafish Alk8 BMP receptor kinase domain fused to VfLOVDepositorInsertAlk8 (acvr1l) kinase domain + VfLOV domain (acvr1l Vaucheria frigida, Zebrafish)
UseTagsExpressionMutationPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
P303-FBP_2
Plasmid#139693PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, without recycling gene CPHDepositorInsertp35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:AtuOCS, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2
Plasmid#87644PurposeFor creating drosophila transgenics expressing UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2DepositorInsertmyr::SNAPf>myr::CLIPf>myr::Halo2
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1(+)-PM-CaNARi
Plasmid#64850PurposePlasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) activation.DepositorInsertLyn-CaNARi
UseTagsCerulean, N-terminal targeting sequence from Lyn …ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
Plas-gRNA-LEU2
Plasmid#157656PurposeGFP and gRNA tag for protein and mRNA quantification, to attache to the 3' end of the gene coding sequenceDepositorInsertGFP-gRNA
UseInsert storage (replicative in e. coli)TagsGFP-gRNAExpressionMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only