We narrowed to 5,627 results for: mcs

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pJL1 sortase

Plasmid

#106288

Purpose

Expresses sortase in Ecoli off a T7 promoter

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

sortase A pentamutant

Expression

Bacterial

Promoter

T7

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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Cyto-GA

Plasmid

#209859

Purpose

Dimerization dependent fluorescent protein GA fused to NES

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddGFP A

Tags

NES sequence LALKLAGLDIGS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pGH020_sgRNA_G418-GFP

Plasmid

#85405

Purpose

hu6 driven sgRNA vector with G418 and GFP selectable markers

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

G418 resistance and GFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pTBL3478 PB-PEmax-mTagBFP2

Plasmid

#226664

Purpose

PiggyBac cargo vector encoding PEmax and mTagBFP2

Depositor

Chang Liu

Article

Loveless et al bioRxiv 2021.11.05.467507

Inserts

PEmax
mTagBFP2

Use

CRISPR

Expression

Mammalian

Promoter

CAG and UBC

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-AAVS1_sgRNA

Plasmid

#183890

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

AAVS1 sgRNA spacer (AAVS1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ14107 fwYellow-targeting sgRNA

Plasmid

#239456

Purpose

guide for fwYellow

Depositor

Stanley Qi

Article

Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

Insert

guide targeting fwYellow

Use

CRISPR; Cell-free system using bacterial extract

Expression

Bacterial

Promoter

J23119

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ14112 tyrosinase-targeting sgRNA

Plasmid

#239751

Purpose

guide for tyrosinase

Depositor

Stanley Qi

Article

Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

Insert

guide targeting tyrosinase

Use

CRISPR; Cell-free system using bacterial extract

Expression

Bacterial

Promoter

J23119

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ14103 aeBlue-targeting_sgRNA

Plasmid

#239750

Purpose

guide for aeBlue

Depositor

Stanley Qi

Article

Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

Insert

guide targeting aeBlue

Use

CRISPR; Cell-free system using bacterial extract

Expression

Bacterial

Promoter

J23119

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pJBL7084

Plasmid

#167248

Purpose

overexpression of LacI

Depositor

Julius Lucks

Article

Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

Insert

pT7-LacI

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pSF-CjPglB

Plasmid

#199305

Purpose

pSN18 derivative encoding C.jejuni PglB with C-terminal FLAG epitope tag

Depositor

Michael Jewett

Article

Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

Insert

CjPglB with flag tag

Tags

Flag tag

Expression

Bacterial

Promoter

pBAD

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-AAVS1_sgRNA

Plasmid

#183891

Purpose

pX459V2.0-HypaCas9 plasmid with sgRNA targeting the AAVS1 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

AAVS1 sgRNA spacer (AAVS1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pNP1 eforRed trigger

Plasmid

#107357

Purpose

Trigger sequence for pNP1 eforRed toehold switch

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

toehold trigger 1

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-SP1

Plasmid

#232649

Purpose

expresses SP1 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

SP1 (SP1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoG-ATP

Plasmid

#193818

Purpose

CMV overexpression of ChemoG-ATP in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG-ATP

Expression

Mammalian

Mutation

EGFP:A206K; HT7: L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pSS9

Plasmid

#71655

Purpose

Contains 600 bp homology arms to SS9 for one-step markerless genome integration in E. coli

Depositor

Ryan Gill

Article

Bassalo et al ACS Synth Biol. 2016 Apr 22.

Insert

gfpuv

Use

Synthetic Biology

Promoter

T7A1

Available Since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoG-CaM

Plasmid

#193816

Purpose

CMV overexpression of ChemoG-CaM in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG-CaM

Expression

Mammalian

Mutation

EGFP:E116D, A206K; HT7: L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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HyWnt3:Luc

Plasmid

#192986

Purpose

Plasmid to test the Hydra Wnt3 promoter activity

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Wnt3 promoter

Expression

Mammalian

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pX459V2.0-HypaCas9-mRuby2

Plasmid

#183872

Purpose

pX459V2.0 plasmid expressing HypaCas9 with P2A-mRuby2 and T2A-Puro, with a sgRNA cloning site for CRISPR editing in mammalian cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Type

Empty backbone

Use

CRISPR

Tags

HypaCas9-P2A-mRuby2-T2A-Puro

Expression

Mammalian

Promoter

Cbh

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoG-NAD

Plasmid

#193821

Purpose

CMV overexpression of ChemoG-NAD in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG-NAD

Expression

Mammalian

Mutation

EGFP:A206K, T225R; HT7: L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pSLQ3538 eforRed-targeting sgRNA

Plasmid

#239455

Purpose

guide for eforRed

Depositor

Stanley Qi

Article

Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

Insert

guide targeting eforRed

Use

CRISPR; Cell-free system using bacterial extract

Expression

Bacterial

Promoter

J23119

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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