We narrowed to 6,675 results for: proc

1. plenti.CamKII.(cyto).iATPSnFR2.A95K.miRFP670nano3

   Plasmid

   #209727

   Purpose

   Expresses cytosolic medium affinity ratiometric ATP sensor

   Depositor

   Timothy Ryan

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   (cyto).iATPSnFR2.A95K.miRFP670nano3
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CamKII

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTara:500

   Plasmid

   #60717

   Purpose

   Expresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORF

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Insert

   T7 RNAP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD

   Available since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac-CHD7

   Plasmid

   #170141

   Purpose

   Expresses CHD7 in insect cells

   Depositor

   Robert Kingston

   Article

   Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

   Insert

   chromodomain helicase DNA binding protein 7 (CHD7 Human)
   

   Use

   Tags

   6xHis and FLAG

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTetOne-mCherry-cNLS

   Plasmid

   #138527

   Purpose

   Inducible expression of mCherry with nuclear localization signal

   Depositor

   Ana Teixeira, Christopher Grigsby

   Article

   Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

   Insert

   mCherry
   

   Use

   Tags

   3x SV40 cNLS

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3Gs

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT-HA-hM3D(Gq)

   Plasmid

   #45547

   Purpose

   Expresses hM3D (Gq) neuronal activator

   Depositor

   Bryan Roth

   Article

   Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

   Insert

   hM3D (Gq)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJFRC201-10XUAS-FRT>STOP>FRT-myr::smGFP-HA

   Plasmid

   #63166

   Purpose

   GAL4-responsive UAS "flp-On" spaghetti monster GFP-HA reporter

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

   Insert

   "spaghetti monster GFP-HA"
   

   Use

   Tags

   N-myristoylation

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. peYFP-C1-mTOR

   Plasmid

   #73384

   Purpose

   Expresses YFP tagged mTOR

   Depositor

   Jie Chen, Taekjip Ha

   Article

   Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

   Insert

   mTOR (MTOR Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiGuide_GFP_NT135

   Plasmid

   #183118

   Purpose

   pLentiGuide modified to express GFP and a non-targeting sgRNA

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   NT135
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV.hSynapsin.(cyto).cpSFGFP.HaloTag

   Plasmid

   #209666

   Purpose

   Non-responsive control

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   cpSFGFP.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSico

    Plasmid

    #11578

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating shRNA expression.

    Depositor

    Tyler Jacks

    Article

    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.HaloTag

    Plasmid

    #209724

    Purpose

    Expresses cytosolic low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (cyto).iATPSnFR2.A95A.A119L.HaloTag
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBI-p53 R175H/EGFP

    Plasmid

    #16544

    Depositor

    Bert Vogelstein

    Article

    Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R175H

    Promoter

    Available since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRGE32

    Plasmid

    #63159

    Purpose

    Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for transient expression in protoplast

    Depositor

    Yinong Yang

    Article

    Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Rice snoRNA U3 promoter for gRNA/PTG expression a…

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCI-neo-mOVA

    Plasmid

    #25099

    Depositor

    Maria Castro

    Article

    Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

    Insert

    membrane bound ovalbumin
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5/FRT-HA-hM4D(Gi)

    Plasmid

    #45548

    Purpose

    Expresses hM4D (Gi) neuronal inhibitor

    Depositor

    Bryan Roth

    Article

    Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

    Insert

    hM4D (Gi)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRK5-HA-YFP-rictor

    Plasmid

    #73387

    Purpose

    Expresses HA and YFP tagged Rictor

    Depositor

    Jie Chen, Taekjip Ha

    Article

    Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

    Insert

    RPTOR Independent Companion Of MTOR, Complex 2 (RICTOR Human)
    

    Use

    Tags

    HA and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Puro.Cre empty vector

    Plasmid

    #17408

    Purpose

    Lentiviral expression of Cre from the mouse PGK promoter

    Depositor

    Tyler Jacks

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

    Insert

    Cre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse PGK

    Available since

    March 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGSKU

    Plasmid

    #72243

    Purpose

    Contains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoter

    Depositor

    Michael Resnick, Francesca Storici

    Article

    Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

    Inserts

    KlURA3
    kanMX4
    I-SceI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-6His-MBP-MpAgo

    Plasmid

    #79249

    Purpose

    This plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expression

    Depositor

    Jennifer Doudna

    Article

    Kaya et al Proc Natl Acad Sci U S A. 2016 Mar 30. p

    Insert

    Marinitoga piezophila Argonaute
    

    Use

    CRISPR

    Tags

    6XHis-tag + MBP + PreScission Cleavage Site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBABE-FLAG-LKB1

    Plasmid

    #8592

    Depositor

    Lewis Cantley

    Article

    Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

    Insert

    LKB1 (STK11 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge