We narrowed to 8,785 results for: sgrna

1. pNTI733 pRPR1(TetO)-RPC31-sgRNA

   Plasmid

   #164913

   Purpose

   For yeast genomic integration of sgRNA against RPC31

   Depositor

   Nicholas Ingolia

   Article

   Muller et al Science. 2020 Dec 11;370(6522). pii: 370

   Insert

   pRPR1(TetO)-RPC31-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   RPR1

   Available Since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNTI732 pRPR1(TetO)-HTS1-sgRNA

   Plasmid

   #164912

   Purpose

   For yeast genomic integration of sgRNA against HTS1

   Depositor

   Nicholas Ingolia

   Article

   Muller et al Science. 2020 Dec 11;370(6522). pii: 370

   Insert

   pRPR1(TetO)-HTS1-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   RPR1

   Available Since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUPD2 sgRNA sCRNA 2.0 (GB2461)

   Plasmid

   #160593

   Purpose

   Version of the native Cas9-sgRNA with one native WT aptamer sequence and F6 aptamer sequence recognized for Ms2 coat protein, in 3'.

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   sgRNA sCRNA 2.0
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-5U6-sgRNAs-hsyn-EGFP

   Plasmid

   #112213

   Purpose

   Targeted DNA methylation

   Depositor

   Xingxu Huang

   Article

   Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

   Insert

   sgRNAs
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19-U6-BsmBI_cassette-St1Cas9-sgRNA (KAC14)

   Plasmid

   #133791

   Purpose

   U6 promoter sgRNA entry vector used for all St1Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V1 sgRNA architecture from Carter et al. biorxiv 2018

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   St1Cas9 sgRNA entry vector
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V1 sgRNA architecture from Carter et al. biorxiv …

   Promoter

   U6

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19-U6-BsmBI_cassette-St3Cas9-sgRNA (KAC27)

   Plasmid

   #133792

   Purpose

   U6 promoter sgRNA entry vector used for all St3Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Muller et al. Molecular Therapy 2015

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   St3Cas9 sgRNA entry vector
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   sgRNA architecture from Muller et al. Molecular T…

   Promoter

   U6

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBXMCS-2 Pconstitutive-sgRNA(Spa)_gcrA3

   Plasmid

   #133343

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets gcrA gene's promoter from Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_gcrA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   constitutive

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA

   Plasmid

   #133345

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets blaA gene's promoter from Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_blaA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   constitutive

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. px330 Gatad2a sgRNA (for flox targeting)

   Plasmid

   #110815

   Purpose

   Used with pNTK Gatad2a flox allele targeting construct in mouse cells

   Depositor

   Jacob Hanna

   Article

   Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

   Insert

   mGataD2a (Gatad2a Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT2K-CAGGS-U6-sgRNA-M7-IRES-CFP

    Plasmid

    #114730

    Purpose

    sgRNA targeting a sequence upstream of the initiator ATG of the cellular Myc gene

    Depositor

    Martine Roussel

    Article

    Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

    Insert

    sgRNA M7
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT2K-CAGGS-U6-sgRNA-M5-IRES-CFP

    Plasmid

    #114728

    Purpose

    sgRNA targeting a sequence upstream of the initiator ATG of the cellular Myc gene

    Depositor

    Martine Roussel

    Article

    Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

    Insert

    sgRNA M5
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA2

    Plasmid

    #133341

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the template strand in Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_gcrA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    constitutive

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX601 miniCMV-SaCas9-SpA-sgRNA scaffold

    Plasmid

    #107055

    Purpose

    Backbone vector for cloning in target sgRNA for use with SaCas9 (SauCas9)

    Depositor

    Alex Hewitt

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE

    Plasmid

    #85452

    Purpose

    Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.

    Depositor

    Aviv Regev

    Article

    Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

    Insert

    U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. B52 (empty plasmid backbone to express 2 sgRNAs)

    Plasmid

    #100708

    Purpose

    Empty plasmid backbone to express 2 sgRNAs (use BbsI and BsmBI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoters

    Available Since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1i

    Plasmid

    #233080

    Purpose

    Expression vector for sgRNA-S1b and eCas12f1i

    Depositor

    Kyoungmi Kim

    Article

    Park et al Nat Commun. 2025 Jan 15;16(1):677. doi:

    Insert

    U6-sgRNA-S1b (BsaI)-Cbh-bpNLS-eCas12f1i-2xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, CBh

    Available Since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6-sgRNA EF1Alpha-puro-T2A-BFP

    Plasmid

    #60955

    Purpose

    Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

    Depositor

    Jonathan Weissman

    Article

    Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

    Inserts

    sgGFP-NT2
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-pCMV-dSaCas9-VP64-pU6-sgRNA

    Plasmid

    #158990

    Purpose

    Vector G encodes pAAV-pCMV-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in mammalian cells

    Depositor

    Chung Tin

    Article

    Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

    Insert

    dSaCas9-VP64
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available Since

    Aug. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-3xsgRNA_Opn1mw-RHO-Cas9N-IntN-polyA

    Plasmid

    #165450

    Purpose

    Express N-terminal part of split dCas9-VPR and 3 sgRNAs targeting murine Opn1mw promoter

    Depositor

    Elvir Becirovic

    Article

    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

    Inserts

    dCas9N-IntN
    3x Opn1mw promoter-targeting sgRNAs
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and short human rhodopsin promoter

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1a

    Plasmid

    #233079

    Purpose

    Expression vector for sgRNA-S1b and eCas12f1a

    Depositor

    Kyoungmi Kim

    Article

    Park et al Nat Commun. 2025 Jan 15;16(1):677. doi:

    Insert

    U6-sgRNA-S1b (BsaI)-Cbh-bpNLS-eCas12f1a-2xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, CBh

    Available Since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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