164,172 results

1. pHes1-DsRed

   Plasmid

   #13767

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   Hes1 promoter
   

   Use

   Tags

   DsRed2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST-CMV-N-mCherry

   Plasmid

   #123215

   Purpose

   N-terminal mCherry tag. Gateway destination vector for mammalian expression.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR-UCOE-EF1a-Zim3-dCas9-P2A-GFP

   Plasmid

   #188778

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Tet-O-FUW-Brn2

   Plasmid

   #27151

   Depositor

   Marius Wernig

   Article

   Vierbuchen et al Nature. 2010 Feb 25. 463(7284):1035-41.

   Insert

   Brn2 (Pou3f2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO-CreERT2 stuffer v3

   Plasmid

   #158046

   Purpose

   lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. Pcr4-12xMBS-PBS

   Plasmid

   #52984

   Purpose

   provide MBS-PBS alternating sequence

   Depositor

   Robert Singer

   Article

   Wu et al Sci Rep. 2014 Jan 9;4:3615. doi: 10.1038

   Insert

   12xMBS-PBS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   none

   Available since

   June 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. L4440_BioBrick-sgRNA

   Plasmid

   #177783

   Purpose

   Overexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegans

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pemiRFP670-tubulin

   Plasmid

   #136573

   Purpose

   Human alpha-tubulin labeling with a near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   emiRFP670-tubulin
   

   Use

   Tags

   emiRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV3flag8HOIL-1L

   Plasmid

   #50016

   Purpose

   Mammalian expression of human HOIL-1L (RBCK1) with a c-terminal 3xFLAG tag, and hygromycin selection.

   Depositor

   Martin Dorf

   Article

   Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

   Insert

   RanBP-type and C3HC4-type zinc finger containing 1 HOIL-1L (RBCK1 Human)
   

   Use

   Tags

   3 x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRT043-CLYBL-DDdcas9-VPH

    Plasmid

    #158091

    Purpose

    Inducible expression of dCas9-VPH from the CLYBL locus for CRISPR activation

    Depositor

    Martin Kampmann

    Article

    Tian et al Nat Neurosci. 2021 May 24. pii: 10.1038/

    Insert

    CLYBL-CAG-DDdCas9VPH-T2A-GFP
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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