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pVAM05

Plasmid

#252056

Purpose

Gentamicin resistant derivative of pKEK2201 for allelic exchange

Depositor

James Bina

Article

Van Allen et al J Microbiol Methods. 2025 Jan;228:107070

Type

Empty backbone

Use

Sucrose counter-selection allelic exchange vector

Available Since

March 13, 2026

Availability

Academic Institutions and Nonprofits only

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pVAM03

Plasmid

#251701

Purpose

Tetracycline resistant derivative of pKEK2201 for allelic exchange

Depositor

James Bina

Article

Van Allen et al J Microbiol Methods. 2025 Jan;228:107070

Type

Empty backbone

Use

Sucrose counter-selection allelic exchange vector

Available Since

March 13, 2026

Availability

Academic Institutions and Nonprofits only

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pTE5426

Plasmid

#229033

Purpose

tetO-phi29 DNA polymerase wild-type reporter: PT7-tetO-B0035-p2 wt-T7 terminator, AmpR

Depositor

Tobias Erb

Article

Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

Insert

PT7-tetO-B0035-phi29 DNAP wt-T7term

Expression

Bacterial

Promoter

PT7-tetO

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pTE5429

Plasmid

#229036

Purpose

Expression vector for EilR: PT7-lacO-B0035-EilR-T7 terminator, AmpR

Depositor

Tobias Erb

Article

Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

Insert

PT7-lacO-RBS35-EilR-T7term

Tags

8x His-tag

Expression

Bacterial

Promoter

PT7-lacO

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW mTagBFP2

Plasmid

#239562

Purpose

Cytosolic expression of mTagBFP2

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mTagBFP2

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1880

Plasmid

#235721

Purpose

Expression of FL yeast VPS15 S622D_H623D_T626E mutant -EGFP under its own promoter in yeast

Depositor

Roger Williams

Article

Špokaitė et al eLife

Insert

VPS15

Expression

Yeast

Mutation

S622D, H623D, T626E

Promoter

ScVPS15 promoter

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only
pBad-MfnG

Plasmid

#191116

Purpose

Expresses MfnG in E. coli

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

MfnG

Expression

Bacterial

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJL1-V3-FETCH1

Plasmid

#210650

Purpose

In vitro expression of V3-FETCH reporter from the T7 promoter

Depositor

Michael Jewett

Article

Willi et al ACS Synth Biol. 2024 Jul 19;13(7):2253-2

Insert

V3-FETCH1

Use

Synthetic Biology

Promoter

T7

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pJL1-FETCH2

Plasmid

#210645

Purpose

In vitro expression of FETCH reporter from the T7 promoter

Depositor

Michael Jewett

Article

Willi et al ACS Synth Biol. 2024 Jul 19;13(7):2253-2

Insert

FETCH2

Use

Synthetic Biology

Promoter

T7

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pDCAF-B

Plasmid

#113647

Purpose

Demethylates methylxanthines.

Depositor

Jeffrey Barrick

Article

Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

Insert

ndmB

Use

Synthetic Biology

Available Since

Nov. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDCAF-C

Plasmid

#113648

Purpose

Demethylates methylxanthines.

Depositor

Jeffrey Barrick

Article

Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

Insert

ndmC

Use

Synthetic Biology

Available Since

Nov. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDCAF-AB

Plasmid

#113649

Purpose

Demethylates methylxanthines.

Depositor

Jeffrey Barrick

Article

Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

Insert

ndmA and ndmB

Use

Synthetic Biology

Available Since

Nov. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDCAF-AC

Plasmid

#113650

Purpose

Demethylates methylxanthines.

Depositor

Jeffrey Barrick

Article

Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

Insert

ndmA and ndmC

Use

Synthetic Biology

Available Since

Nov. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDCAF-BC

Plasmid

#113651

Purpose

Demethylates methylxanthines.

Depositor

Jeffrey Barrick

Article

Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

Insert

ndmB and ndmC

Use

Synthetic Biology

Available Since

Nov. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDCAF-A

Plasmid

#113646

Purpose

Demethylates methylxanthines.

Depositor

Jeffrey Barrick

Article

Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

Insert

ndmA

Use

Synthetic Biology

Available Since

Nov. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pPS1069

Plasmid

#1354

Depositor

Pamela Silver

Article

Seedorf et al Proc Natl Acad Sci U S A 1997 Aug 5;94(1

Insert

PSE1 (PSE1 Budding Yeast)

Tags

sGFP

Expression

Yeast

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pFLP3

Plasmid

#64946

Purpose

Flp recombinase expression vector

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

Flp recombinase

Expression

Bacterial

Promoter

lambda promoter/temperature-sensitive Lambda cI r…

Available Since

Nov. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1(+) mCherry

Plasmid

#176301

Purpose

Expresses mCherry mRNA from CMV promoter

Depositor

Jeremy Wilusz

Article

Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

Insert

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pUC18R6KT-mini-Tn7T-Km

Plasmid

#64969

Purpose

mini-Tn7 base vector with transcriptional terminator and oriT

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pUC18-mini-Tn7T-Gm-lux

Plasmid

#64963

Purpose

mini-Tn7 luxCDABE transcriptional fusion vector

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

lux operon

Expression

Bacterial

Available Since

Nov. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCRISPR

Plasmid

#42875

Purpose

A crRNA expression plasmid for targeting a specific sequence.

Depositor

Luciano Marraffini

Article

Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

CRISPR-BsaI

Use

CRISPR; E.coli

Expression

Bacterial

Available Since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

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pET24c(+)-EFTu

Plasmid

#31172

Depositor

Linda Spremulli

Article

Zhang et al FEBS Lett. 1996 Aug 12. 391(3):330-2.

Insert

E. coli elongation factor Tu (tufB E. coli)

Tags

His

Expression

Bacterial

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pFUW COX8ASig-mCherry

Plasmid

#239558

Purpose

Expression and mitochondrial localization of mCherry

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mCherry

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-MfnG

Plasmid

#191115

Purpose

Lentiviral vector expressing MfnG

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

MfnG

Use

Lentiviral

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pFUW LAMP1Sig-mEmerald

Plasmid

#239555

Purpose

Expression and lysosomal localization of mEmerald

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mEmerald

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW LAMP1Sig-mCherry

Plasmid

#239556

Purpose

Expression and lysosomal localization of mCherry

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mCherry

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pDonor_ShCAST_kanR

Plasmid

#127924

Purpose

Kanamycin resistance donor for ShCAST

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

KanR, R6K origin of replication

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pDDGFP_LEU2D

Plasmid

#58352

Purpose

Used for expressing recombinant proteins as a C terminal GFP-His tagged constuct in S.cerevisiae

Depositor

Simon Newstead

Article

Parker et al Protein Sci. 2014 Jun 20. doi: 10.1002/p

Type

Empty backbone

Tags

GFP-His

Expression

Yeast

Promoter

GAL1

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pMSCV-loxp-dsRed-loxp-3xHA-Puro-WPRE

Plasmid

#32703

Depositor

Hans Clevers

Article

Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

Type

Empty backbone

Use

Cre/Lox and Retroviral

Tags

3x HA tags

Expression

Mammalian

Available Since

Jan. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pUC18-mini-Tn7T-LAC

Plasmid

#64965

Purpose

mini-Tn7 vector with Ptac expression cassette

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 14, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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