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pNCST-avGFP

Plasmid

#129508

Purpose

Expresses avGFP (Lambert 2019 clone) constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

avGFP

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-BICD2

Plasmid

#164626

Purpose

Expresses BICD2 in mammalian cells with a GFP tag

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

BICD2 (BICD2 Human)

Tags

Cycle 3 GFP

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-JIP4

Plasmid

#164620

Purpose

Expresses JIP4 in mammalian cells with a GFP tag

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

JIP4 (SPAG9 Human)

Tags

cycle 3 GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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KIF1A(1-393)-GCN4-2xmCherry-EF(C)

Plasmid

#61665

Purpose

Tandem mCherry-labeled, forced-dimeric, truncated kinesin-3 fused to EF Hand linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF1A(1-393)-GCN4-2xmCherry-EF(C) (KIF1A Human)

Expression

Mammalian

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-JIP4

Plasmid

#164621

Purpose

Expresses JIP4 in mammalian cells with a mNeonGreen tag

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

JIP4 (SPAG9 Human)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pODN-mNG-ANLN

Plasmid

#183834

Purpose

Repair template for the N-terminal tagging of anillin with mNeonGreen in human cells using CRISPR/Cas9.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

ANLN homology arms with mNeonGreen-linker (ANLN Human)

Use

CRISPR; Donor template

Tags

mNeonGreen-linker

Expression

Mammalian

Mutation

Homology arms contain point mutations to remove t…

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pME-DreERT2 (JDW 1344)

Plasmid

#224523

Purpose

A Gateway-compatible middle entry clone containing Dre recombinase fused to mutant ERT2 for tamoxifen-inducible recombinase activity

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

DreERT2

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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FAT FAK biosensor

Plasmid

#78303

Purpose

FRET biosensor. Visualization of FAK activity at focal adhesions in live cells

Depositor

Yingxiao Wang

Article

Seong et al Proc Natl Acad Sci U S A. 2013 Nov 26;11

Insert

FAT FAK biosensor

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2016

Availability

Academic Institutions and Nonprofits only
pAAV.CAG.(ER).iGlucoSnFR2.HaloTag

Plasmid

#244073

Purpose

ER expression of green glucose sensor with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.HaloTag

Use

AAV

Tags

Calreticulin and HaloTag

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(mem).iGlucoSnFR2

Plasmid

#244077

Purpose

Plasma membrane targeted expression of green glucose sensor

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Tags

IgG kappa leader

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(ER).iGlucoSnFR2.H348H.HaloTag

Plasmid

#244074

Purpose

ER expression of green glucose sensor (high affinity) with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.H348H.HaloTag

Use

AAV

Tags

Calreticulin and HaloTag

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iGlucoSnFR2.H348H.HaloTag

Plasmid

#244070

Purpose

Cytosolic expression of green glucose sensor (high affinity) with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.H348H.HaloTag

Use

AAV

Tags

HaloTag

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.TBG.(cyto).iGlucoSnFR2.mIRFP670nano3

Plasmid

#244103

Purpose

Cytosolic expression of green glucose sensor with non-responsive mIRFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Use

AAV

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-RAB35

Plasmid

#164630

Purpose

Expresses RAB35 in mammalian cells with a GFP tag

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

RAB35 (RAB35 Human)

Tags

cycle 3 GFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 22, 2021

Availability

Academic Institutions and Nonprofits only

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p5E-mmCdh5pro (JDW 1239)

Plasmid

#224478

Purpose

Gateway compatible 5' entry clone containing the Murine Cdh5 promoter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Cdh5 promoter

Expression

Mammalian

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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2xmyc-JIP4

Plasmid

#164633

Purpose

Expresses JIP4 in mammalian cells with a 2xmyc tag

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

JIP4 (SPAG9 Human)

Tags

2xmyc

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pNCST-AvicFP1

Plasmid

#129504

Purpose

Expresses AvicFP1 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AvicFP1

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pNCST-AvicFP4

Plasmid

#129507

Purpose

Expresses AvicFP4 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AvicFP4

Expression

Bacterial

Mutation

One of two variants of AvicFP4 tested with essent…

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pNCST-AvicFP2

Plasmid

#129505

Purpose

Expresses AvicFP2 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AvicFP2

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1-mTagBFP2-rMPC1 shRNA

Plasmid

#229016

Purpose

Expression of an shRNA construct that knocks down rat MPC1. This plasmid also encodes a blue fluorescent protein tag to verify transfection

Depositor

Ghazaleh Ashrafi

Article

Tiwari et al Sci Adv. 2024 Nov 15;10(46):eadp7423. do

Insert

MPC1 (Mpc1 Rat)

Use

Lentiviral

Tags

mTagBFP2

Expression

Mammalian

Promoter

U6

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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