We narrowed to 1,827 results for: PORA

1. pmCherry-NES-OptoJNKi

   Plasmid

   #89739

   Purpose

   Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to cytoplasm, wild-type photosensor, inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   NLS-OptoJNKi
   

   Use

   Fluorescent protein

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKCAG-H2BmCherry-OptoJNKi

   Plasmid

   #89741

   Purpose

   Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to chromatin with H2B, wild-type photosensor, inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   OptoJNKi
   

   Use

   Optogenetics

   Tags

   Histone2B and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAG 442TAA

   Plasmid

   #154779

   Purpose

   plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAG 277TAA, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   LaG17-SynNotch
   

   Use

   Tags

   LaG17

   Expression

   Mammalian

   Mutation

   204TAG 442TAA in LaG17-SynNotch, hybrid PylT with…

   Promoter

   EF1

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEN_TTmiRc2_BirA_CDKN1A

   Plasmid

   #136523

   Purpose

   Used as a donor vector to clone into pSLIK

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   CDKN1A (CDKN1A Human)
   

   Use

   Entry vector for gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBEST-p70a-UTR1-Nterm 6xHis mRFP-T500

   Plasmid

   #92316

   Purpose

   Expression of NTerm 6xHis tagged mRFP using the p70a promoter and UTR1

   Depositor

   Lital Alfonta

   Article

   Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

   Insert

   Optimized NTerm 6xHis tagged monomeric RFP (mRFP)
   

   Use

   Synthetic Biology; Cell free protein synthesis (c…

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   p70a

   Available since

   Aug. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-Hygro-TRE>hHES3:T2A:EGFP

   Plasmid

   #236630

   Purpose

   Hygromycin selected lentiviral construct with tetracyclin-inducible human HES3

   Depositor

   Genevieve Kendall

   Article

   Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10

   Inserts

   HES3 (HES3 Human)
   EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Codon optimized based on a variant of wildtype GF…

   Promoter

   TRE

   Available since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag-APEX2-SOPP3-3xNLS

   Plasmid

   #229074

   Purpose

   expresses APEX2-SOPP3 in the mammalian nucleus

   Depositor

   Weijun Pan

   Article

   Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

   Insert

   Express APEX2-SOPP3 in the mammalian nucleus (APEX2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. COX8mls-Flag-APEX2-SOPP3

   Plasmid

   #229075

   Purpose

   Expresses APEX2-SOPP3 in the mitochondrial matrix

   Depositor

   Weijun Pan

   Article

   Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

   Insert

   Express APEX2-SOPP3 in the mitochondrial matrix (APEX2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR1A 2xHA GPR137

   Plasmid

   #226809

   Purpose

   Gateway entry vector for GPR137

   Depositor

   Ramiro Iglesias-Bartolome

   Article

   Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

   Insert

   GPR137 (GPR137 Human)
   

   Use

   Gateway entry

   Tags

   2xHA tag followed by a GDPPVAT linker

   Expression

   Mutation

   Sequence optimized from NP_001164351

   Promoter

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMRXIP-GFP-TAPP1(PHD)

    Plasmid

    #221757

    Purpose

    Stable expression of GFP-TAPP1(PHD) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    PLEKHA1 (PLEKHA1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    tandem TAPP1 (PHD) (amino acids 184-304)

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRXIP-Btk1(PHD)-GFP

    Plasmid

    #221756

    Purpose

    Stable expression of Btk1(PHD)-GFP in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    Btk (BTK Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    amino acids 1–177

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMRXIP-GFP-ING2(PHD)

    Plasmid

    #221751

    Purpose

    Stable expression of GFP-ING2(PHD) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    ING2 (ING2 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    two ING2(PlantHD) domains (amino acids 190–280)

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. SSH-pSUMO-β′-COPI-WD40-Tyr33Ala

    Plasmid

    #208170

    Purpose

    Bacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40-Tyr33Ala mutant (residues 1-301)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

    Insert

    N-terminal domain of yeast β′-COPI-WD40 subunit (1-301) with Tyr33Ala substitution
    

    Use

    Tags

    His-tag, SUMO-tag, and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Glu

    Plasmid

    #218461

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Glu substitution)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

    Insert

    SARS-CoV-2 spike protein (1273 residues) carrying Thr1273Glu substitution (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Thr1273AGlu substitution

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Asp

    Plasmid

    #218462

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Asp substitution)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

    Insert

    SARS-CoV-2 spike protein (1273 residues) carrying Thr1273Asp substitution (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Thr1273Asp substitution

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-RepoMan-RATA

    Plasmid

    #184270

    Purpose

    Expresses shRNA resistant GFP-RepoMan RATA Mutant

    Depositor

    Lienhard Schmitz

    Article

    Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

    Insert

    cell division cycle associated 2 (CDCA2 Human)
    

    Use

    Tags

    3xHA and GFP

    Expression

    Mammalian

    Mutation

    V383A, F395A

    Promoter

    CMV

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-RepoMan-T394A

    Plasmid

    #204994

    Purpose

    Expresses shRNA resistant GFP-RepoMan T394A Mutant

    Depositor

    Lienhard Schmitz

    Article

    Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

    Insert

    cell devision cycle associated 2 (CDCA2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    changed Threonine 394 to Alanin

    Promoter

    CMV

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pROS_phleo-Tps1/Gsy2

    Plasmid

    #196612

    Purpose

    Encoding guide RNAs for the knock out of TPS1 and GSY2 genes

    Depositor

    Matthias Heinemann

    Article

    Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

    Insert

    TPS1, GSY2 (TPS1, GSY2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pROS13-Tps2/Gsy1

    Plasmid

    #196613

    Purpose

    Encoding guide RNAs for the knock out of TPS2 and GSY1 genes

    Depositor

    Matthias Heinemann

    Article

    Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

    Insert

    S000001911 (TPS2, GSY1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUGP1.1

    Plasmid

    #196615

    Purpose

    Plasmid used for the C-terminal tagging of Ugp1 with mCherry and the auxin-inducible degron in yeast

    Depositor

    Matthias Heinemann

    Article

    Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

    Insert

    UGP1::mCherry-AID(71-114)-NatMX (UGP1 Budding Yeast)
    

    Use

    Tags

    AID(71-114) and mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Native promoter

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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