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Venus Cam KII alpha 1-315aa

Plasmid

#29431

Depositor

Steven Vogel

Article

Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

Insert

Venus CAM KII Alpha

Tags

Venus

Expression

Mammalian

Mutation

Expresses CamKIIa amino acids 1-315

Available Since

July 5, 2011

Availability

Academic Institutions and Nonprofits only
phtsResA

Plasmid

#25914

Depositor

Johann Deisenhofer

Article

Colbert et al Proc Natl Acad Sci U S A. 2006 Mar 21. 1

Insert

His-tagged soluble ResA (resA Bacillus subtilis)

Tags

His

Expression

Bacterial

Mutation

Start at Serine 37 to remove n-terminal transmemb…

Available Since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only
PCDNA3 T7 DLC2

Plasmid

#24268

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

DLC2 (Dynll2 Mouse)

Tags

T7

Expression

Mammalian

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pRS416 Gal-RNQ1 L94A

Plasmid

#18691

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 L94A

Expression

Yeast

Mutation

Leucine 94 mutated to Alanine (L94A)

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pLIC.B4.TM1717

Plasmid

#11532

Depositor

Sung-Hou Kim

Article

Shin et al Proc Natl Acad Sci U S A. 2004 Sep 7. 10

Insert

YjeQ GDP binding protein (TM1717 T. maritima)

Tags

HisTag-MBP, TEV cleavable

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
p031 pCS2 End-1 ORF

Plasmid

#11030

Depositor

Ramesh Shivdasani

Article

Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

Insert

End-1 (end-1 Nematode)

Use

Xenopus, mammalian, avian, and zebrafish

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pPS834

Plasmid

#8932

Depositor

Pamela Silver

Article

Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

Insert

GSP1 (GSP1 Budding Yeast)

Tags

myc

Expression

Yeast

Available Since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
BcLOV4-mammalian-recoded_mCherry_pcDNA3.1

Plasmid

#114595

Purpose

Encodes mCherry-tagged BcLOV4 for expression in mammalian cells

Depositor

Brian Chow

Article

Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

Insert

BcLOV4

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pUB-GFP

Plasmid

#11155

Purpose

Mammalian expression vector for expression of GFP (Ubiquitin C promoter)

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Has Service

Cloning Grade DNA

Insert

Ubiquitin C promoter (UBC Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT-HA-hM3D(Gq)

Plasmid

#45547

Purpose

Expresses hM3D (Gq) neuronal activator

Depositor

Bryan Roth

Article

Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

Insert

hM3D (Gq)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

July 3, 2013

Availability

Academic Institutions and Nonprofits only

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pSUB11

Plasmid

#169699

Purpose

Template plasmid for generating C-terminal 3xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.

Depositor

Nara Figueroa

Article

Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

Insert

3xFLAG tag adjacent to FRT-flanked aph (KanR) gene

Tags

3xFLAG tag

Expression

Bacterial

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.miRFP670nano3

Plasmid

#209723

Purpose

Expresses mitochondrially targeted low affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(mito).iATPSnFR2.A95A.A119L.miRFP670nano3

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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Luciferase-pcDNA3

Plasmid

#18964

Depositor

William Kaelin

Article

Safran et al Proc Natl Acad Sci U S A. 2006 Jan 3. 10

Insert

Firefly Luciferase

Expression

Mammalian

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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sortase A pentamutant (eSrtA) in pET29

Plasmid

#75144

Purpose

evolved sortase A (eSrtA) pentamutant with improved kinetics and activity

Depositor

David Liu

Article

Chen et al Proc Natl Acad Sci U S A. 2011 Jul 12;10

Insert

sortase A pentamutant

Tags

6x His

Expression

Bacterial

Mutation

P94R, D160N, D165A, K190E, K196T. Deleted amino a…

Promoter

T7

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-LKB1

Plasmid

#8590

Depositor

Lewis Cantley

Article

Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

Insert

LKB1 (STK11 Human)

Tags

FLAG

Expression

Mammalian

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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pJL NarX Myc

Plasmid

#225651

Purpose

Expresses WT NarX capable of sensing nitrate. For use with NarL-YdfI and reporter plasmids from this study.

Depositor

Neha Kamat

Article

Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

Insert

NarX (narX E. coli)

Use

Cell free expression

Tags

Myc

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-Rab7A Q86L

Plasmid

#28050

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Insert

Rab7A (RAB7A Human)

Tags

2xHA and 3xFlag

Expression

Mammalian

Mutation

Q86L

Available Since

June 22, 2011

Availability

Academic Institutions and Nonprofits only
CMV-Hylight

Plasmid

#193447

Purpose

A fluorescent biosensor for fructose 1,6-bisphosphate (FBP). Changes in FBP concentration strongly correlate with changes in glycolytic flux. (mammalian expression vector)

Depositor

Richard Goodman

Article

Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

Insert

Hylight Biosensor

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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SpyCatcher003 S49C

Plasmid

#133448

Purpose

Expresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical coupling

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyCatcher003 S49C

Tags

GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Mutation

Contains S49C mutation

Promoter

T7

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

Plasmid

#236406

Purpose

Expresses cytosolic low affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

May 23, 2025

Availability

Academic Institutions and Nonprofits only

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