We narrowed to 14,071 results for: cas9

1. pEJS1027-pMCSG7-SmuCas9

   Plasmid

   #121541

   Purpose

   Expresses a 6X-His tagged type II-C Cas9 from S. muelleri in bacterial cells

   Depositor

   Erik Sontheimer

   Article

   Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

   Insert

   SmuCas9
   

   Expression

   Bacterial

   Available Since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-TAC-OKMS-Cas9

   Plasmid

   #120353

   Purpose

   piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Insert

   OKMS cassette
   

   Use

   CRISPR; Piggybac transposon

   Expression

   Mammalian

   Mutation

   Klf4 [10-483]

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. PX458-3xHA-SpCas9

   Plasmid

   #130968

   Purpose

   3xHA tagged Cas9 from S. pyogenes with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

   Insert

   3xHA-NLS-SpCas9
   

   Use

   CRISPR

   Tags

   3x HA, EGFP, and NLS

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Nov. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST-APIC-Cas9

   Plasmid

   #121657

   Purpose

   gateway cloning destination vector for expressing enhancer driven Cas9

   Depositor

   Chun Han

   Article

   Poe et al Genetics. 2018 Nov 30. pii: genetics.118

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFREE2-xCas9-sgRNA

   Plasmid

   #179579

   Purpose

   Expresses xCas9 and gRNA that targets ColE1 plasmids for curing.

   Depositor

   Kevin Solomon

   Article

   Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

   Insert

   xCas9
   

   Expression

   Bacterial

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. A3D-Cas9n-UGI

   Plasmid

   #119137

   Purpose

   Base editor made from APOBEC3D

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   APOBEC3D-Cas9 nickase-UGI
   

   Use

   CRISPR

   Mutation

   None

   Promoter

   CMV

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS415-Cas9-VPR

   Plasmid

   #163971

   Purpose

   Trifunctional Cas9-VPR fusion protein plasmid for simultaneous transcriptional activation, transcriptional repression, and genome editing in yeast

   Depositor

   Jiazhang Lian

   Article

   Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

   Insert

   Cas9-VPR
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. ciCas9(F22)_pcDNA5

   Plasmid

   #100552

   Purpose

   Expresses ciCas9(F22) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

   Insert

   ciCas9(F22)
   

   Use

   CRISPR; Frt, flp-in

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. V1-MESA-45F-M-dCas9

   Plasmid

   #84504

   Purpose

   MESA target chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, M cleavage sequence, and dCas9-VP64

   Depositor

   Joshua Leonard

   Article

   Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

   Insert

   V2-MESA-35F-M-dCas9
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. SpyCas9 PE2-3HA

    Plasmid

    #169853

    Purpose

    Mammalian Expression, SpyCas9 prime editor with 3HA-tag

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    SpCas9-H840A-M-MLV-3HA
    

    Tags

    3XHA tag and bpSV40 NLS

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV dCas9-MC (eGFP)

    Plasmid

    #89931

    Purpose

    Expresses the dCas9-MC fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.

    Depositor

    Carl Novina

    Article

    Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

    Insert

    dCas9-MC
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deactivated Cas9, fragment of M.SssI (residues 27…

    Promoter

    CMV

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. eSpCas9(1.1)_No_FLAG_ATP1A1-G7

    Plasmid

    #173203

    Purpose

    Expresses the ATP1A1 G7 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 17. pX330-like plasmid.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G7 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX459V2.0-SpCas9-HF4

    Plasmid

    #108295

    Purpose

    pX459 V2.0 (Plasmid #62988) with the Y450A, N497A, R661A, Q695A, and Q926A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-Flag-dHeFSpCas9

    Plasmid

    #92116

    Purpose

    Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive HeFSpCas9 with FLAG tag
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

    Promoter

    Cbh

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBLO 9.2_bCas9-CP199

    Plasmid

    #121496

    Purpose

    TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    p15A

    Available Since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. A3F-Cas9n-UGI

    Plasmid

    #119138

    Purpose

    Base editor made from APOBEC3F

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    APOBEC3F-Cas9 nickase-UGI
    

    Use

    CRISPR

    Mutation

    None

    Promoter

    CMV

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. A3C-Cas9n-UGI

    Plasmid

    #119136

    Purpose

    Base editor made from APOBEC3C

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    APOBEC3C-Cas9 nickase-UGI
    

    Use

    CRISPR

    Mutation

    None

    Promoter

    CMV

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. zCREST3:Cas9green; T3_gRNA

    Plasmid

    #199335

    Purpose

    pDEL161; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type III sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    zCREST3
    Cas9
    nrg1 type III sgRNA
    

    Use

    Tol2 destination vector

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330 NC-FKBP-Cas9

    Plasmid

    #162725

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#2

    Plasmid

    #107730

    Purpose

    Knock out of EB3 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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