We narrowed to 7,391 results for: ef1a;

1. pLenti-EF1a-SPdCas9-DNMT3A(E752A)-2A-Blast

   Plasmid

   #71218

   Purpose

   dCas9 fused to the human DNMT3A catalytically inactive domain

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-DNMT3A(E752A)
   

   Use

   Lentiviral

   Tags

   SPdCas9-DNMT3A(E752A)

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-EF1a-CDK6-mutPAM-PGK-puro

   Plasmid

   #187638

   Purpose

   sg resistant CDK6 ORF

   Depositor

   William Kaelin

   Article

   Nicholson et al Sci Signal. 2019 Oct 1;12(601). pii: 12/

   Insert

   CDK6 (CDK6 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   PAM site mutation

   Promoter

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSIN-U6-tracer_C9_1_-EF1a-Thy1.1-P2A-Neo

   Plasmid

   #191397

   Purpose

   sgRNA targeting alpha-satellites on human chromosome 9

   Depositor

   Albert Jeltsch

   Article

   Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

   Insert

   sgRNA targeting alpha satellites on human chromosome 9
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCF525-EF1a-Hygro-P2A-AcrVA4-lenti

   Plasmid

   #115663

   Purpose

   Expresses AcrVA4 in human cells, contained in a lentiviral vector.

   Depositor

   Jennifer Doudna

   Article

   Watters et al Science. 2018 Oct 12;362(6411):236-239.

   Insert

   AcrVA4
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF-1a promoter

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-ActA-IRES-puromycin-pLVx-EF1a

   Plasmid

   #177433

   Purpose

   To express mCherry fused to ActA mitochondria targeting sequence. Lentiviral vector used to make cell lines expressing this mitochondria landmark.

   Depositor

   David Andrews

   Insert

   ActA tail anchor (actA Listeria monocytogenes)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-DIO hChR2(E123A)-mCherry

   Plasmid

   #35508

   Purpose

   AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Insert

   hChR2
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E123A

   Promoter

   Ef1a

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-DIO-hBFP-2A-RVG-WPRE

   Plasmid

   #120268

   Purpose

   Permits retrograde transsynaptic spread by driving Cre-dependent co-expression of the RbG plus a nuclear reporter

   Depositor

   Edward Callaway

   Article

   Faget et al Cell Rep. 2016 Jun 21;15(12):2796-808. d

   Insert

   EF1α-DIO-H2B-tagBFP-Flagx3-T2Am-cB19G
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-mir144 EF1Alpha-puro-T2A-BFP

   Plasmid

   #164791

   Purpose

   Express miR-144 under the U6 promoter in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   miR-144 (MIR144 Human)
   puro-T2A-BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1Alpha and U6

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-EF1a-GFP-SFPQY527A-IRES-mCherry

   Plasmid

   #166951

   Purpose

   Lentiviral plasmid expressing GFP-tagged SFPQ Y527A protein with IRES-mCherry from the EF1a promoter

   Depositor

   Rosalind Segal

   Article

   Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

   Insert

   SFPQ-Y527A (SFPQ Human)
   

   Use

   Lentiviral

   Tags

   GFP and mCherry

   Expression

   Mutation

   SFPQ-Y527A

   Promoter

   eF1a

   Available Since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Ef1a-Con/Fon/Von GCaMP 6m

    Plasmid

    #137165

    Purpose

    Intersectional viral expression of GCaMP6M in cells expressing Cre AND Flp AND Vcre

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Insert

    3x-GCaMP6M
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    Removed RSET tag

    Promoter

    Ef1a

    Available Since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Ef1a-Con/Foff 2.0-BFP

    Plasmid

    #137130

    Purpose

    Intersectional viral expression of BFP in cells expressing Cre AND NOT Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-BFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1

    Plasmid

    #164800

    Purpose

    For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    ERH CRISPRi gRNA1
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha and U6

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2

    Plasmid

    #164801

    Purpose

    For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    ERH CRISPRi gRNA2
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha and U6

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. NFATc2-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75232

    Purpose

    CRISPR/Cas9 plasmid against human NFATc2

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc2 (NFATC2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. NFATc1-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75236

    Purpose

    CRISPR/Cas9 plasmid against human NFATc1

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc1 (NFATC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCherry-Cb5-IRES-puromycin-pLVx-EF1a

    Plasmid

    #177434

    Purpose

    To express mCherry fused to Cb5 endoplasmic reticulum (ER) targeting sequence. Lentiviral vector used to make cell lines expressing this ER landmark.

    Depositor

    David Andrews

    Insert

    Cb5 tail anchor (Cyb5r4 Rat)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE2-EF1a-MCS-dOB-hPOT1_IRES-blast

    Plasmid

    #166413

    Purpose

    express hPOT1 ΔOB in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

    Insert

    Protection Of Telomeres 1 (POT1 Human)
    

    Use

    Lentiviral

    Tags

    myc

    Expression

    Mammalian

    Mutation

    ΔOB deleted 125 a.a

    Promoter

    ef1-a

    Available Since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-Ef1a-FAS-ChETA-TdTomato-WPRE-pA

    Plasmid

    #37089

    Purpose

    Expresses ChETA-tdTomato in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic excitation

    Depositor

    Bernardo Sabatini

    Article

    Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

    Insert

    ChETA-TdTomato
    

    Use

    AAV and Cre/Lox; Cre-off

    Tags

    TdTomato

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    July 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-pEF1a-FLEX-HA-VHH-KASH-WPRE

    Plasmid

    #129704

    Purpose

    Express HA-KASH for nuclei labeling and purification

    Depositor

    Yi Zhang

    Article

    Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

    Insert

    HA-VHH-KASH
    

    Use

    AAV and Cre/Lox

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCF525-EF1a-Hygro-P2A-AcrVA1-lenti

    Plasmid

    #115662

    Purpose

    Expresses AcrVA1 in human cells, contained in a lentiviral vector.

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    AcrVA1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a promoter

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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