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Plasmid#59995PurposeInserts mCherry immediately downstream of the Nanog coding regionDepositorInsertNanog (Nanog Mouse)
UseMouse TargetingTags2A-mCherryExpressionMutationPromoterAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPbU6_hdhfr/yfcu_Cas9
Plasmid#216423PurposeEmpty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter and the Cas9 nuclease for traditional CRISPR editing.DepositorTypeEmpty backboneUseCRISPR; Expression in plasmodium bergheiTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPbHiT_3xmyc_hsp70UTR_hdhfr/yfcu
Plasmid#216421PurposeEmpty backbone to express gene specific gRNA and carry the homology repair template for CRISPR editing of Plasmodium berghei using the PbHiT system.DepositorTypeEmpty backboneUseCRISPR; Expression in plasmodium bergheiTags3xmycExpressionBacterialMutationPromoterAvailable sinceApril 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPbU6_hdhfr/yfcu
Plasmid#216422PurposeEmpty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter for traditional CRISPR editing.DepositorTypeEmpty backboneUseCRISPR; Expression in plasmodium bergheiTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNANOG-Luc
Plasmid#25900DepositorInsertNANOG promoter (NANOG Human)
UseTagsLuciferaseExpressionMammalianMutationNonePromoterAvailable sinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGL2- Empty control -SV40-Luc
Plasmid#26280PurposeAddgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.DepositorTypeEmpty backboneUseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
TRE-TIGHT-EGFP-backward donor
Plasmid#22077DepositorInsertTETO-eGFP
UseTagsExpressionMutationPromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBRPyCAG-hKlf2-DsRed-Ip
Plasmid#26277DepositorInsertKlf2 (KLF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2010AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 SA-2A-puro-pA donor
Plasmid#22075PurposeAAV zinc finger donor vector for genomic targetingDepositorInsertSA-Puro
UseZfn donorTagsExpressionMutationPromoterAvailable sinceOct. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAGGS-EGFP
Plasmid#22212PurposeAAV zinc finger donor vector for genomic targetingDepositorInsertCAGGS-eGFP
UseZfn donorTagsExpressionMutationPromoterAvailable sinceOct. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 hPGK-PuroR-pA donor
Plasmid#22072DepositorInsertPGK-Puro (PGK1 Human)
UseZfn donorTagsExpressionMutationPromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTRE-TIGHT-EGFP-donor fw copy
Plasmid#22074DepositorInsertTETO-eGFP
UseTagsExpressionMutationPromoterAvailable sinceSept. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFrt-invCAG-ires-Luc
Plasmid#63576PurposeShuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional cDNA and Luc expression upon integration. The vector contains an FRT site and two mut loxP sites.DepositorInsertStop-Lox71-IRES-Luciferase-pA-FRT-Lox66-reverseCAG
UseCre/Lox; Frt/flpe recombination mediated insertionTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCol1a1-frt (FVB)
Plasmid#63575PurposeTargeting vector for the Col1a1 locus that contains a Neo resistance cassette flanked by FRT sites, followed by an ATG-less Hygromycine resistance gene. The homology arms match the sequence of FVB.DepositorInsertCol1A-frt-hygro-pA
UseMouse Targeting; Frt/flpeTagsExpressionBacterial and MammalianMutationPromoterNoneAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPB-tetpA-hNEUROG2-iRPT
Plasmid#140764PurposeExpresses iRFP713, PuroR and rtTAM2 in mammalian cells, with TRE-hNEUROG2DepositorInsertsUseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBRPyCAG-fmKlf4-DsRed-Ip
Plasmid#26274DepositorInsertKlf4 (Klf4 Mouse)
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
Sox2-SE-RGM-eGFP targeting vector
Plasmid#133045PurposeTargeting vector of RGM-eGFP with homologous arms around Sox2 super-enhancer DMRDepositorInsertSox2-SE-5arm-RGM-Sox2-SE-3arm
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFrt-invCAG-Luc
Plasmid#63577PurposeShuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional Luciferase expression upon integration. The vector contains an FRT site and two mutant loxP sitesDepositorInsertStop-Lox71-Luciferase-pA-FRT-Lox66-reverseCAG
UseCre/Lox; Frt/flpe recombination mediated insertionTagsExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
miR290-SE-RGM-eGFP targeting vector
Plasmid#133046PurposeTargeting vector of RGM-eGFP with homologous arms around Mir290 super-enhancer DMRDepositorInsertmiR290-SE-5arm-RGM-miR290-SE-3arm
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Ctr1-gRNA-EGFP
Plasmid#226000PurposeNegative control for CRISPRi-KDDepositorInsertnegtive control sgRNA of no specific targets in human genome
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Ctr2-gRNA-EGFP
Plasmid#226001PurposeNegative control for CRISPRi-KDDepositorInsertnegtive control sgRNA of no specific targets in human genome
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-hL1-gRNA1-EGFP
Plasmid#226002PurposeCRISPRi-KD of human LINE1DepositorInsertsgRNA targeting human L1HS
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-hL1-gRNA2-EGFP
Plasmid#226003PurposeCRISPRi-KD of human LINE1DepositorInsertsgRNA targeting human L1HS
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
miR290-SE-RGM-tdTomato targeting vector
Plasmid#133047PurposeTargeting vector of RGM-tdTomato with homologous arms around Mir290 super-enhancer DMRDepositorInsertmiR290-SE-5arm-RGM-miR290-SE-3arm
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_ISG
Pooled Library#125753PurposeExpresses gRNAs targeting human Interferon-Stimulated GenesDepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMuSIC pool v1.0
Pooled Library#227193PurposeTwo-way barcodes from 18 fluorescent proteins for mammalian expression. Can be used for clone tracing, or for pairing with perturbations for single cell resolution in spectral detection measurements.DepositorExpressionMammalianAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
plIG-123_HA-GD2-28z_CAR_TF_Library
Pooled Library#207478PurposeHA-GD2-28z CAR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into thDepositorSpeciesHomo sapiensUseCRISPRAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
plIG-128_HA-GD2-28z_CAR_TFxTF_Library
Pooled Library#207480PurposeHA-GD2-28z CAR in combination with ~10,000 transcription factor combinations (and related proteins) and controls (GFP/RFP combinations). Library can be used to generate HDR templates for modular pooleDepositorUseCRISPRAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
plIG-106_NY-ESO-1_TCR_SR_Library
Pooled Library#207474PurposeNY-ESO-1 TCR in combination with 129 surface receptors and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the TRAC locus of human T cellsDepositorSpeciesHomo sapiensUseCRISPRAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
plIG-107_NY-ESO-1_TCR_TF_Library
Pooled Library#207475PurposeNY-ESO-1 TCR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into theDepositorSpeciesHomo sapiensUseCRISPRAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
plIG-109_CD19-BBz_CAR_SR_Library
Pooled Library#207476PurposeCD19-BBz CAR in combination with 129 surface receptors and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the TRAC locus of human T cellsDepositorUseCRISPRAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
plIG-110_CD19-BBz_CAR_TF_Library
Pooled Library#207477PurposeCD19-BBz CAR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into theDepositorSpeciesHomo sapiensUseCRISPRAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
plIG-124_HA-GD2-28z_CAR_SR_Library
Pooled Library#207479PurposeHA-GD2-28z CAR in combination with 129 surface receptors and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the TRAC locus of human T celDepositorSpeciesHomo sapiensUseCRISPRAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
plIG-132_CD19-28z_CAR_TF_and_SR_Library
Pooled Library#207481PurposeCD19-28z CAR in combination with 129 surface receptors, 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knocDepositorSpeciesHomo sapiensUseCRISPRAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNR2E3-donor
Plasmid#176369PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertNR2E3 (NR2E3 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2-hUbC-dCas9-KRAB-T2a-Puro
Plasmid#225999PurposeTo establish stable dCas9-KRAB knock-in lineDepositorInserthumanized dCas9-KRAB T2A Puro
UseSleeping beauty transposonTagsExpressionMammalianMutationD10A and H840APromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
OCT4 GFP puro Donor 1
Plasmid#22209DepositorInsertSA-OCT-GFP-2APuro-PA (POU5F1 Human)
UseTagsExpressionMutationPromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
TV-hPITX3-forward
Plasmid#22073DepositorInsertPITX3 (ASMD Human)
UseCre/Lox; Zfn donorTagseGFPExpressionMutationPromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
OCT4 GFP puro Donor 2
Plasmid#22210DepositorInsertSA-OCT-GFP-2APuro-PA (POU5F1 Human)
UseTagsExpressionMutationPromoterAvailable sinceOct. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
OCT4 GFP puro Donor 3
Plasmid#22211DepositorInsertSA-OCT-GFP-2APuro-PA (POU5F1 Human)
UseTagsExpressionMutationPromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only