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Plasmid#16522DepositorInsertFAST-1 H83R (FOXH1 Human)
UseTagsExpressionMammalianMutationH83R mutantPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAG500-FKBP-N-frFAST
Plasmid#160060PurposeExpresses FKBP-N-frFAST in mammalian cellsDepositorInsertFKBP-N-frFAST
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac K-M33-Flag
Plasmid#1993DepositorInsertM33 (Cbx2 Mouse)
UseTagsFlagExpressionInsectMutationKozak sequence added before ATGPromoterAvailable sinceMarch 23, 2006AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-RS06
Plasmid#52647Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-RS06
UseTagsExpressionMammalianMutationM374Q mutated from GCaMP3PromoterT7Available sinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-RS14
Plasmid#52651Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-RS14
UseTagsExpressionMammalianMutationdeleted G47 from GCaMP3PromoterT7Available sinceMay 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pfast bac Rbx-HA
Plasmid#51893PurposeBackbone plasmid is pfastBacHTa from invitrogenDepositorInsertRbx1 (RBX1 Human)
UseTagsHAExpressionMutationPromoterpolyhedrin promoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-chNfasc (+RSLE)
Plasmid#89418PurposeTwo-hybrid systemDepositorInsertNFASC (NFASC Chicken)
UseTagsGAL4 DBDExpressionYeastMutationPromoterADH1Available sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac His HA GNU WT
Plasmid#112994PurposeBaculovirus expression of His/HA-tagged Drosophila gnuDepositorInsertgnu (gnu Fly)
UseTagsHis/HAExpressionInsectMutationPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-RS12
Plasmid#52650Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-RS12
UseTagsExpressionMammalianMutationdeleted R40/R41/K42 from GCaMP3PromoterT7Available sinceMay 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-LacI-LAP
Plasmid#114034PurposeProduction of baculovirus with LacI-LAPDepositorInsertLacI-LAP
UseTagsExpressionInsectMutationnonePromoterCMVAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Mel18 flag
Plasmid#1969DepositorInsertMel18 (Pcgf2 Mouse)
UseTagsflagExpressionInsectMutationPromoterAvailable sinceMarch 23, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFasBac+GFP-CAMSAP2 CC
Plasmid#59040Purposefor baculovirus expression of CAMSAP2 CC in Sf9 cellsDepositorInsertCAMSAP2 CC domain (CAMSAP2 Human)
UseTagsHis and eGFPExpressionInsectMutationPromoterAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-EF09
Plasmid#52640Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-EF09
UseTagsExpressionMammalianMutationD366N mutation from GCaMP3 sequencePromoterT7Available sinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-hDcr-K70A.
Plasmid#89145PurposeExpression of helicase mutant of human Dicer in Sf9 cellsDepositorInserthDicer (DICER1 Human)
UseTagsHis6-tevExpressionInsectMutationK70APromoterAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFasBac+GFP-CAMSAP3 CC
Plasmid#59042Purposefor baculovirus expression of mouse CAMSAP3 CC in Sf9 cellsDepositorInsertCAMSAP3 CC domain (Camsap3 Mouse)
UseTagsHis and eGFPExpressionInsectMutationPromoterAvailable sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
FASTK gRNA (BRDN0001144738)
Plasmid#77876Purpose3rd generation lentiviral gRNA plasmid targeting human FASTKDepositorInsertFASTK (FASTK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FASTK gRNA (BRDN0001145485)
Plasmid#77877Purpose3rd generation lentiviral gRNA plasmid targeting human FASTKDepositorInsertFASTK (FASTK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-EF15
Plasmid#52641Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-EF15
UseTagsExpressionMammalianMutationD431N/D433N/D435N mutated from GCaMP3PromoterT7Available sinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
OMM-short-CFAST10
Plasmid#233597PurposeExpression of CFAST10 on the OMM membrane after a short linkerDepositorInsertCFAST10
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
ER-long-RspA-NFAST
Plasmid#233600PurposeExpression of RspA-NFAST on the ER membrane after a 55 aminoacid linkerDepositorInsertRspA-NFAST
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
STIM1-RspA-NFAST
Plasmid#233606PurposeExpression of hSTIM1-RspA-NFASTDepositorInsertSTIM1 (STIM1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFastBac SOX R139A
Plasmid#192058PurposeExpression vector for SOX (ORF37) R139ADepositorInsertSOX (ORF37)
UseTagsExpressionInsectMutationR139APromoterPolyhedrinAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac SOX Q129H
Plasmid#192057PurposeExpression vector for SOX (ORF37) Q129HDepositorInsertSOX (ORF37) Q129H
UseTagsExpressionInsectMutationQ129HPromoterPolyhedrinAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-NEDD4L_ΔC2 (157)
Plasmid#187720PurposeExpress GST-tagged NEDD4L ΔC2 (157) in insect cellsDepositorInsertNEDD4L residues 157-975 (NEDD4L Human)
UseTagsGST-HRV 3C siteExpressionInsectMutationdeletion of residues 1-156PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBacHTA-NEDD4L_C2-W1-L-W2-HECT
Plasmid#187723PurposeExpress His-tagged NEDD4L C2-W1-L-W2-HECT chimera in insect cellsDepositorInsertNEDD4L residues 1-418+582-975 (NEDD4L Human)
UseTagsHis-TEVExpressionInsectMutationdeletion of residues 419-581PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBacHTA-NEDD4L_W1-L-W2-HECT
Plasmid#187724PurposeExpress His-tagged NEDD4L W1-L-W2-HECT chimera in inserct cellsDepositorInsertNEDD4L residues 153-418+582-975 (NEDD4L Human)
UseTagsHis-TEVExpressionInsectMutationdeletion of residues 1-152 and 419-581PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBacI-KDAC6 H216A
Plasmid#224351PurposeExpresses human KDAC6 (HDAC6) H216A in insect cellsDepositorInsertKDAC6 (HDAC6 Human)
UseTagsTEV-cleavable His6ExpressionInsectMutationHistidine 216 mutated to alaninePromoterPolyhedrinAvailable sinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBacI-KDAC6 H611A
Plasmid#224352PurposeExpresses human KDAC6 (HDAC6) H611A in insect cellsDepositorInsertKDAC6 (HDAC6 Human)
UseTagsTEV-cleavable His6ExpressionInsectMutationHistidine 611 mutated to alaninePromoterPolyhedrinAvailable sinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBac-GST-TBK1
Plasmid#221331PurposeTBK1 protein overexpression in Sf9 insect cellsDepositorInsertTBK1 (Tbk1 Mouse)
UseTags6xHis-TEV-2xGST-TEVExpressionInsectMutationPromoterpolyhedrinAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
ER-FAST-IRES-iRFP670
Plasmid#216344PurposeFor testing localization of pFAST and iRFP670 proteins to endoplasmic reticulumDepositorInsertPRL_pFAST-IRES-CANX_iRFP670
UseTagsmyc for FAST and iRF670 proteinsExpressionMammalianMutationn/aPromoterCMVAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NFAST(low)-Halo-ER
Plasmid#214419PurposeER localization of low-affinity NFAST fused to Halo-ERDepositorInsertRspA-N
UseTagsHaloExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
CFAST-Halotag-6xHp
Plasmid#214416PurposeLipid droplet localization of CFAST fused to Halotag-6xHpDepositorInsertdeletion
UseTagsCFAST-HaloExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-C17orf53-Strep
Plasmid#211446Purposegeneration of Bacmid for insect cell expression of C17orf53 C-terminally fused to Strep-TagDepositorInsertC17orf53 (HROB Human)
UseTagsStrep-TagExpressionInsectMutationPromoterAvailable sinceMarch 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-C17orf53-FLAG
Plasmid#211447Purposegeneration of Bacmid for insect cell expression of C17orf53 C-terminally fused to FLAG-TagDepositorInsertC17orf53 (HROB Human)
UseTagsFLAG-TagExpressionInsectMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-bullfrog saxiphilin
Plasmid#140595Purposeprotein expressionDepositorInsertsaxiphilin bullfrog
UseTagsGFP-His10ExpressionInsectMutationDeletion of M20 - please see depositor commentPromoterpolyhedrin promoterAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-M.Fas.1
Plasmid#196687PurposeRep/Cap plasmid for the production of M.Fas.1, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationTDALTTK insert between amino acids 588 and 589 of…Promoterp41Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-M.Fas.4
Plasmid#196690PurposeRep/Cap plasmid for the production of M.Fas.4, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationVVSDYTV insert between amino acids 588 and 589 of…Promoterp41Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-M.Fas.3
Plasmid#196689PurposeRep/Cap plasmid for the production of M.Fas.3, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationRVDPSGL insert between amino acids 588 and 589 of…Promoterp41Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-M.Fas.2
Plasmid#196688PurposeRep/Cap plasmid for the production of M.Fas.2, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationSTIPTMK insert between amino acids 588 and 589 of…Promoterp41Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac FAK W266A
Plasmid#186144PurposeFor expresseion of recombinant FAK using baccolovirus in SF9 cellsDepositorInsertPTK2 (PTK2 Mouse)
UseTagsHis6ExpressionInsectMutationDNA mutation T796G;G797C Amino acid mutation W266APromoterpolyhedrin promoterAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only