We narrowed to 13,832 results for: cas9

1. Sa-SlugCas9

   Plasmid

   #163797

   Purpose

   Expresses Sa-SlugCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. SlutrCas9

   Plasmid

   #163795

   Purpose

   Expresses SlutrCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas9cur

   Plasmid

   #71538

   Purpose

   Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

   Depositor

   Tao Chen

   Article

   Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

   Inserts

   Cas9
   Plasmid curing system
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pScCas9

   Plasmid

   #117700

   Purpose

   Mammalian expression vector encoding WT ScCas9

   Depositor

   Joseph Jacobson

   Article

   Chatterjee et al Sci Adv. 2018 Oct 24;4(10):eaau0766. doi

   Insert

   Streptococcus canis Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCgCas9_recT

   Plasmid

   #153337

   Purpose

   Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; Kanr

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. SpCas9

   Plasmid

   #188489

   Purpose

   Expresses FLAG tagged SpCas9

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCas9

   Plasmid

   #167547

   Purpose

   Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

   Depositor

   Howard Hang

   Article

   Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

   Inserts

   chloramphenicol acetyl transferase
   pUC19 origin of replication
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBBR_Cas9_sp1

   Plasmid

   #154851

   Purpose

   Expresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genome

   Depositor

   John van der Oost

   Article

   Mougiakos et al Microb Cell Fact. 2019 Nov 25;18(1):204.

   Inserts

   Spcas9 gene
   sgRNA scaffold
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Mutation

   Codon harmonized variant for expression in Rhodob…

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS42H_PTEF1_dCas9_TCYC1

   Plasmid

   #177155

   Purpose

   Expresses dCas9 in yeast cells

   Depositor

   Gautam Dantas

   Article

   Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

   Insert

   Nuclease-null Cas9
   

   Use

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   TEF1 promoter

   Available since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSaCas9_GFP

    Plasmid

    #64709

    Purpose

    Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells

    Depositor

    Kiran Musunuru

    Insert

    SaCas9-2A-GFP
    

    Use

    CRISPR

    Tags

    2A-GFP and 3xFLAG-SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. p15A_SpyCas9_CmR

    Plasmid

    #119162

    Purpose

    Expresses S. pyogenes Cas9

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    Cas9 from S. pyogenes (NEWENTRY )
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCas9CyMutL

    Plasmid

    #166981

    Purpose

    Encodes aTc-inducible spCas9 and cumate-inducible MutLE32K for transient suppression of mismatch repair

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Inserts

    MutL (mutL )
    CymR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    E32K

    Promoter

    pCymRC and pLacI

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDual_dsCas9_BFP

    Plasmid

    #214679

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-BFP with two empty sgRNA cloning sites

    Depositor

    Kevin Janes

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Nme2Cas9_pMCSG7

    Plasmid

    #119925

    Purpose

    Nme2Cas9 bacterial expression plasmid

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    Nme2Cas9
    

    Use

    Tags

    NLS, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. BlatCas9

    Plasmid

    #163792

    Purpose

    The plasmid expresses human codon-optimized BlatCas9 and gRNA expression elements.

    Depositor

    Yongming Wang

    Article

    Gao et al Front Cell Dev Biol. 2020 Oct 19;8:58316

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. NAX_CAGGS_CAS9_m4_VPR_EBFP2

    Plasmid

    #167881

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. SpCas9-NG

    Plasmid

    #138566

    Purpose

    Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    SpCas9-NG
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…

    Promoter

    EFS

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAS9i

    Plasmid

    #141249

    Purpose

    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Article

    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 (Cas9), pSNR52 (gRNA)

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. NAX_CAGGS_CAS9_Hygro

    Plasmid

    #167859

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HypaCas9

    Plasmid

    #138557

    Purpose

    Expresses human codon-optimized hyper-accurate SpCas9 and blasticidin resistance: EFS promoter-HypaCas9-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    HypaCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    N692A, M694A, Q695A, H698A

    Promoter

    EFS

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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