Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 13,877 results for: ckb

Clear keywords
Showing: 121 - 140 of 13877 results

  5. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid
    #73797
    Purpose
    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73797-plasmid-map-sequence-id-196924

    Enlarge

  12. TDP-REGv2(mScarlet-FLAG)#10 in pTwist-CMV backbone

    Plasmid
    #216154
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. Leaky but very sensitive to mild TDP-43 loss of function. It uses a cryptic exon. [Code 'B5'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic exon and C-terminal FLAG
    Expression
    Mammalian
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216154-plasmid-map-sequence-id-437765

    Enlarge

  16. TDP-REGv2(mScarlet-FLAG)#7 in pTwist-CMV backbone

    Plasmid
    #216152
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. This plasmid has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells. It uses a cryptic exon. [Code 'A11'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic exon and C-terminal FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216152-plasmid-map-sequence-id-437762

    Enlarge

  17. TDP-REGv2(mScarlet-FLAG)#9 in pTwist-CMV backbone

    Plasmid
    #216153
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. Has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells (brighter than #7). It uses a single intron. [Code 'B11']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic splice site and C-terminal FLAG tag
    Expression
    Mammalian
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216153-plasmid-map-sequence-id-437757

    Enlarge

Showing: 121 - 140 of 13877 results