Showing: 121 - 140 of 1159 results
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Plasmid#86667Purposefor generation of AID strains using CRISPR tagging technologyDepositorInsertLinker-AID-Ty-HGPRT 3'UTR
UseExpression in toxoplasma gondiiTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLinker-AID-3xHA-DHFR-LoxP
Plasmid#86669Purposefor generation of AID strains using CRISPR tagging technologyDepositorInsertLinker-AID-3xHA-HXGPRT 3'UTR
UseExpression in toxoplasma gondiiTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMSR
Plasmid#161983PurposeAllele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile 630. Uses inducible expression of CD2517.1 toxin as a counter-selection markerDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMSR0
Plasmid#161984PurposeAllele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile ribotype 027 and other ribotypes. Uses inducible expression of CD2517.1 toxin as a counter-selection markerDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pU6-DHFR
Plasmid#80329PurposeAllows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamineDepositorInsertDHFR-TSc3
UseCRISPRTagsExpressionMutationPromoterDHFR-TS 5' UTRAvailabilityAcademic Institutions and Nonprofits only -
pLoxP-DHFR-mCherry
Plasmid#70147PurposeExpresses a DHFR-mCherry fusion protein and uses for a CRISPR gene deletionDepositorInserta selectable dihydrofolate reductase-thymidylate synthase marker
UseExpression in toxoplasma gondiiTagsmCherryExpressionMutationPromoterDHFR 5' UTRAvailabilityAcademic Institutions and Nonprofits only -
pCAT-YFP-TetR
Plasmid#59018PurposeExpresses a fusion of yellow fluorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondiiDepositorInsertYFP-TetR
UseToxoplasma expressionTagsExpressionMutationPromoteralpha-tubulinAvailabilityAcademic Institutions and Nonprofits only -
pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP
Plasmid#171090PurposeCRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.DepositorInsertsSth1 dCas9
Chloramphenicol acetyltransferase
TagBFP
UseCRISPR; Toxoplasma gondii expressionTags3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…ExpressionMutationD9A, H599APromoterTUB1 (Toxoplasma gondii), transcriptionally linke…AvailabilityAcademic Institutions and Nonprofits only -
pCS2.aBTX
Plasmid#122257PurposeExpress membrane-tethered alpha-bungarotoxin in Danio rerioDepositorInsertalpha-bungarotoxin
UseSynthetic BiologyTagsFLAGExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLinker-2xTy-HXGPRT-LoxP
Plasmid#86664PurposeCRISPR tagging with 2xTy in Toxoplamsa gondiiDepositorInsertLinker-2xTy-HXGPRT 3'UTR
UseExpression in toxoplasma gondiiTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCVD002
Plasmid#63688Purposefor use in sequencing and mapping of the V. cholerae enterotoxin genesDepositorInsertenterotoxin genes
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pccGFPKAN
Plasmid#73649PurposeFluorescent reporter for transmembrane protein dimerization.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBHDuet40
Plasmid#124358PurposeIPTG-inducible vector encoding the N-terminal gp41-1 split fragment with N-terminally His-tagged GB1DepositorInsertgp41-1 N-intein
UseTagsHis6-GB1ExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pBHBAD42
Plasmid#124359PurposeArabinose-inducible vector encoding the C-terminal gp41-1 split fragment fused to GB1DepositorInsertgp41-1 C-intein
UseTagsGB1 added during cloningExpressionBacterialMutationPromoterParaAvailabilityAcademic Institutions and Nonprofits only -
pccGFPGpA
Plasmid#73651PurposeFluorescent reporter for transmembrane protein dimerization positive control.DepositorInsertglycophorin A
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pccGFPG83I
Plasmid#73650PurposeFluorescent reporter for transmembrane protein dimerization negative control.DepositorInsertglycophorin A- G83I
UseTagsExpressionBacterialMutationglycine 83 to isoleucinePromoterAvailabilityAcademic Institutions and Nonprofits only -
pFW11-ffluc
Plasmid#128487PurposeAllelic exchange plasmid to to integrate firefly luciferase gene into S. pyogenes for biophotonic imagingDepositorInsertfirefly luciferase gene
UseAllelic replacement vectorTagsspy0535 downstream flanking region and spy0535 up…ExpressionMutationPromotersyntheticAvailabilityAcademic Institutions and Nonprofits only -
pCVD402
Plasmid#66148Purposeto make a probe to detect Heat stable enterotoxin gene (human)DepositorInsertSTh
UseCloning vectorTagsExpressionMutationPromoternoneAvailabilityAcademic Institutions and Nonprofits only -
pCVD404
Plasmid#66149Purposeto make a probe to detect Heat stable enterotoxin gene (porcine)DepositorInsertSTp
UseCloning vectorTagsExpressionMutationPromoternoneAvailabilityAcademic Institutions and Nonprofits only -
pLIC-SM-HA
Plasmid#111188PurposeHigh affinity HA Tagging of weakly expressed genes (optimized for Toxoplasma gondii)DepositorTypeEmpty backboneUseToxoplasma gondii expressionTagsSpaghetti Monster HA TagExpressionMutationPromoterEndogenousAvailabilityIndustry, Academic Institutions, and Nonprofits
Showing: 121 - 140 of 1159 results