We narrowed to 4,529 results for: GCA

1. ATP1A1_G6_T804N_MTOR_E2419K_Dual_pegRNA

   Plasmid

   #178110

   Purpose

   Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-E2419K pegRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G6 T804N pegRNA + MTOR-E2419K pegRNA
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pORANGE Cacnb4-GFP KI

   Plasmid

   #139663

   Purpose

   Endogenous tagging of CaVβ4: C-terminal (amino acid position: H417)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSECB sgMTHFD2L_1

   Plasmid

   #106306

   Purpose

   Express Cas9 and sgRNA targeting MTHFD2L

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   sgRNA targeting MTHFD2L (MTHFD2L Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AURKA A2.1 gRNA

   Plasmid

   #90541

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human AURKA

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   AURKA (Guide Designation A2.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLKO.1-blast-cfTuba_1

   Plasmid

   #26717

   Depositor

   Keith Mostov

   Article

   Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

   Insert

   dog Tuba RNAi (DNMBP C. familiaris (dog RNAi))
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Dog Tuba RNAi

   Promoter

   Available since

   Dec. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

6. PX458_CEBPA_2

   Plasmid

   #86359

   Purpose

   Encodes gRNA for 3' target of human CEBPA

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against CEBPA (CEBPA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLJM1-SLC35B2-FLAG

   Plasmid

   #154863

   Purpose

   Transient or Lentiviral expression of sgRNA (Addgene#154860) resistant SLC35B2

   Depositor

   Yihong Ye

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

   Insert

   SLC35B2 (SLC35B2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   ctcctggtgcagtacttc => ttattagtccaatatttt

   Promoter

   CMV

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SRSF2 A9.3 gRNA

   Plasmid

   #90902

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SRSF2

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   SRSF2 (Guide Designation A9.3)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLKO.1-blast-cfTuba_2

   Plasmid

   #26718

   Depositor

   Keith Mostov

   Article

   Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

   Insert

   dog Tuba RNAi (DNMBP C. familiaris (dog RNAi))
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Canine Tuba_2 RNAi

   Promoter

   Available since

   Dec. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1(+)-MC38-TCRB-33-MC_hammer

    Plasmid

    #138990

    Purpose

    IVT template for the beta subunit of the mouse TCR #33 that is reactive against MC38

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    TCRB (Tcrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1(+)-MC38-TCRB-32-riot_punisher

    Plasmid

    #138988

    Purpose

    IVT template for the beta subunit of the mouse TCR #32 that is reactive against MC38

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    TCRB (Tcrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1(+)-MC38-TCRA-32-riot_punisher

    Plasmid

    #138987

    Purpose

    IVT template for the alpha subunit of the mouse TCR #32 that is reactive against MC38

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    TCRA (Tcra Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1(+)-MC38-TCRB-31-murderous_crow

    Plasmid

    #138986

    Purpose

    IVT template for the beta subunit of the mouse TCR #31 that is reactive against MC38

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    TCRB (Tcrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX330_ATP1A1_G3_Dual_sgRNA

    Plasmid

    #173205

    Purpose

    Coselection for HDR in human cells. Vector for dual expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G3 sgRNA + user-specified sgRNA + SpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual U6 promoters

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRP_8xgRNA-FLuc

    Plasmid

    #135937

    Purpose

    Firefly luciferase reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minCMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a7

    Plasmid

    #124859

    Purpose

    Mutagenesis of Slc17a7

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a7 (Slc17a7 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CDC6 B5.1 gRNA

    Plasmid

    #90596

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDC6

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    CDC6 (Guide Designation B5.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ORC1 G2.1 gRNA

    Plasmid

    #90821

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ORC1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    ORC1 (Guide Designation G2.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PX458_CEBPG_2

    Plasmid

    #86345

    Purpose

    Encodes gRNA for 3' target of human CEBPG

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against CEBPG (CEBPG Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ECT2 D6.1 gRNA

    Plasmid

    #90673

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ECT2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    ECT2 (Guide Designation D6.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. EPHB4-gRNA1/2

    Plasmid

    #139974

    Purpose

    gRNA used for knockin EGFP into EPHB4 allele

    Depositor

    James Thomson

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

    Insert

    EPHB4 gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pORANGE GFP-Cacnb1 KI

    Plasmid

    #139660

    Purpose

    Endogenous tagging of CaVβ1: N-terminal (amino acid position: S8)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. MSH6 E8.3 gRNA

    Plasmid

    #90773

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MSH6

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    MSH6 (Guide Designation E8.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MISP H9.2 gRNA

    Plasmid

    #90770

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MISP

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    MISP (Guide Designation H9.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pENTR-TER+-shLKB1-Ms

    Plasmid

    #61234

    Purpose

    Entry clone encoding shRNA to mouse LKB1

    Depositor

    Reuben Shaw

    Article

    Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

    Insert

    shLkb1 (Ms/Hu) (STK11, Stk11 Human, Mouse)
    

    Use

    Entr clone

    Tags

    Expression

    Mutation

    Promoter

    H1/TO

    Available since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

26. MAPRE1 F9.3 gRNA

    Plasmid

    #90746

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAPRE1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    MAPRE1 (Guide Designation F9.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. ZW10 H10.3 gRNA

    Plasmid

    #90943

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ZW10

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    ZW10 (Guide Designation H10.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MAD2L1 E11.1 gRNA

    Plasmid

    #90742

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAD2L1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    MAD2L1 (Guide Designation E11.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CDT1 B10.1 gRNA

    Plasmid

    #90609

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDT1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    CDT1 (Guide Designation B10.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. SASS6 G2.3 gRNA

    Plasmid

    #90885

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SASS6

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    SASS6 (Guide Designation G2.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. LentiCRISPRv2-sgSORCS2-G2

    Plasmid

    #118416

    Purpose

    CRISPR knockout, expresses Cas9, and sgRNA targeting Human SORCS2

    Depositor

    Mark Denham

    Article

    Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

    Insert

    gRNA SORCS2 Human (SORCS2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLentiCRISPR sgFTCD_1

    Plasmid

    #102312

    Purpose

    genetic depletion of FTCD

    Depositor

    David Sabatini

    Article

    Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    sgFTCD_1 (FTCD Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. gh173

    Plasmid

    #106834

    Purpose

    expression of gRNA targeting UGGT2

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    UGGT2 (UGGT2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. AURKB A3.4 gRNA

    Plasmid

    #90546

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human AURKB

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    AURKB (Guide Designation A3.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. INCENP G3.3 gRNA

    Plasmid

    #90712

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human INCENP

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    INCENP (Guide Designation G3.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. UBA1 G6.3 gRNA

    Plasmid

    #90933

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human UBA1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    UBA1 (Guide Designation G6.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. psiCHECK-2-Rhoc-m_3'UTR

    Plasmid

    #31885

    Depositor

    Robert Blelloch

    Article

    Subramanyam et al Nat Biotechnol. 2011 May;29(5):443-8. Ep

    Insert

    Rhoc mutant 3'UTR (RHOC Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    miR-302b, 372 7-mer seed match mutated from AGCAC…

    Promoter

    SV40

    Available since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

38. HJURP E4.1 gRNA

    Plasmid

    #90711

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human HJURP

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    HJURP (Guide Designation E4.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. NXT1 A5.3 gRNA

    Plasmid

    #90814

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NXT1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    NXT1 (Guide Designation A5.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. SGO1 H1.1 gRNA

    Plasmid

    #90886

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SGO1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    SGO1 (Guide Designation H1.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge