We narrowed to 10,531 results for: Plat;
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Plasmid#63143PurposePCR template to construct polycistronic tRNA-gRNADepositorInsertgRNA-tRNA fusion
UseCRISPR and Synthetic BiologyAvailable SinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
T7-CMVtrans-FFLuc-polyA
Plasmid#101156PurposeTemplate for T7 RNAP production of RNA transcript for expression of firefly luciferase in a eukaryotic mammalian cell free lysate.DepositorInsertFirefly luciferase
UseLuciferasePromoterT7 promoterAvailable SinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJH136
Plasmid#47554PurposepJH136 carries a URA3 cassette flanked by PCR priming sites U2 and D2. URA3 was derived from S288c and extends from 243 bp upstream of the start codon to 79 bp downstream of the stop codon.DepositorInsertURA3
UsePcr templatePromoterNative URA3 promoter, -243 to -1.Available SinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGH224_sgRNA_2xMS2_Puro
Plasmid#85413PurposehU6 driven sgRNA vector with 2xMS2 vectors with puro selectable markerDepositorInsertpuromycin resistance
UseCRISPR and LentiviralExpressionMammalianAvailable SinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-52: HIST1H2BJ-mEGFP
Plasmid#109121PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human HIST1H2BJDepositorInsertHIST1H2BJ Homology Arms with linker-mEGFP (H2BC11 Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceMay 4, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
AICSDP-35:AAVS1-mEGFP
Plasmid#91565PurposeHomology arms and linker-mEGFP sequence for internal insertion of CAGGS-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFPDepositorInsertAAVS1 Homology Arms with CAGGS driven mEGFP (AAVS1 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterCAGAvailable SinceJune 7, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
AICSDP-58: NUP153-mEGFP
Plasmid#114407PurposeHomology arms and mEGFP-linker sequence for N-terminus tagging of human NUP153DepositorInsertNUP153 Homology Arms with mEGFP-linker (NUP153 Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceSept. 5, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
AICSDP-26:ST6GAL1-mEGFP
Plasmid#101786PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human ST6GAL1DepositorInsertST6GAL1 Homology Arms with linker-mEGFP (ST6GAL1 Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceOct. 31, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
AICSDP-39:RAB5A-mEGFP
Plasmid#107579PurposeHomology arms and mEGFP-linker sequence for N-terminus tagging of human RAB5ADepositorInsertRAB5A Homology Arms with mEGFP-linker (RAB5A Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceMay 9, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pUC_sgRNA
Plasmid#68710PurposeContains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyAvailable SinceSept. 14, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAG25-L-IFP-F[1] (clon 3)
Plasmid#52899PurposePlasmid template for PCR of cassettes for HR, used to introduce IFP F[1] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.DepositorInsertL-IFP-F[1]
UseVector for homologous recombinationPromoterTEF promoterAvailable SinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG32-L-IFP-F[2] (clon 3)
Plasmid#52900PurposePlasmid template for PCR of cassettes for HR, used to introduce IFP F[2] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.DepositorInsertL-IFP-F[2]
UseVector for homologous recombinationPromoterTEF promoterAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMOTag-M9-4H
Plasmid#106379PurposeCan be used as a PCR template to amplify the M9-HA point mutant of glmS fragment for gene specific tagging that only allows tagging but not regulation. Acts as a control for glmS based gene regulationDepositorInsertGlmS with M9 mutation
Available SinceMarch 2, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSNAPf-AP
Plasmid#61830PurposeEncodes SNAP-AP fusion for tagging endogenous target for SNAP-SiMPullDepositorInsertSNAP-AP
UsePcr templateExpressionBacterial and YeastAvailable SinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-mPlexinD1
Plasmid#16424DepositorAvailable SinceDec. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMGC
Plasmid#112812PurposePCR template vector for amplifying gRNA(F+E)-OstRNA fragmentDepositorInsertgRNAcore(F+E)-tRNA
ExpressionBacterialAvailable SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19 gene 60 thru gap
Plasmid#49160PurposeTemplate for generating gene 60 mRNA truncated two codons after coding gapDepositorInsertBacteriophage T4 gene 60
Mutationgene 60 mRNA truncated two codons after coding ga…PromoterT7Available SinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19 gene 60 to gap + Cys
Plasmid#49159PurposeTemplate for generating gene 60 mRNA, sequence 5' of coding gap + CysDepositorInsertBacteriophage T4 gene 600
MutationCoding gap and downstream sequence have been dele…PromoterT7Available SinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pComb3XLambda
Plasmid#63892PurposeBackbone pComb3X containing PCR template for human antibody lambda light chain constant regionDepositorInserthuman antibody lambda light chain (IGLL5 Human)
UsePhage displayExpressionBacterialPromoterlacZAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only