We narrowed to 15,974 results for: grna

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pML107-LUG1

Plasmid

#226265

Purpose

Plasmid expressing Cas9 and gRNA TCTTCAAGTTACTCCAAGAG which targets the LUG1 gene

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

LUG1 gRNA (YLR352W Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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CAN1 gRNA HIS

Plasmid

#182521

Purpose

Expression vector for gRNA directed against CAN1 ORF.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

CAN1 gRNA (CAN1 Budding Yeast)

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pOC1 Halo-Actb KI

Plasmid

#183426

Purpose

CreON knock-in for Halo-beta-actin (amino acid position: before start codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and Halo donor

Use

CRISPR

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pEE834

Plasmid

#231168

Purpose

T-DNA encoding TRV2 with mobile gRNA targeting SlPDS

Depositor

Daniel Voytas

Article

Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

Insert

mobile gRNA targeting SlPDS

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-NT-1mer-gRNA

Plasmid

#227500

Purpose

non-targeting control guide

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

Non-targeting gRNA

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pLsCas13a-gRNA-1

Plasmid

#164865

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for LsCas13a in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

LsCas13a-gRNA-1

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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PX458_KLF9_2

Plasmid

#86347

Purpose

Encodes gRNA for 3' target of human KLF9

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KLF9 (KLF9 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_KLF9_1

Plasmid

#86346

Purpose

Encodes gRNA for 3' target of human KLF9

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KLF9 (KLF9 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBluescriptSKII+ U6-sgRNA(F+E) ACTB

Plasmid

#74705

Purpose

Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of ACTB mRNA

Depositor

Eugene Yeo

Article

Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

Insert

U6 promoter driving sgRNA targeting the 3'UTR of ACTB mRNA

Use

CRISPR

Expression

Mammalian

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti gRNA KO OCLNx2 spCas9-T2A-iRFP670-P2A-puro

Plasmid

#208399

Purpose

codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, iRFP670 fluorescent protein and puromycin resistance

Depositor

Raphael Gaudin

Article

Pradel et al Autophagy. 2024 Aug;20(8):1825-1836. doi

Insert

hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)

Use

CRISPR and Lentiviral

Tags

iRFP670

Expression

Mammalian

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti gRNA KO OCLNx2 spCas9-T2A-Crimson-P2A-puro

Plasmid

#208398

Purpose

codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, E2-Crimson fluorescent protein and puromycin resistance

Depositor

Raphael Gaudin

Article

Pradel et al Autophagy. 2024 Aug;20(8):1825-1836. doi

Insert

hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)

Use

CRISPR and Lentiviral

Tags

E2-Crimson

Expression

Mammalian

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti gRNA KO OCLNx2 spCas9-T2A-mNeonGreen-P2A-puro

Plasmid

#208400

Purpose

codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, mNeonGreen fluorescent protein and puromycin resistance

Depositor

Raphael Gaudin

Article

Pradel et al Autophagy. 2024 Aug;20(8):1825-1836. doi

Insert

hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)

Use

CRISPR and Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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PasCas13b-gRNA-EGFP(W58stop)

Plasmid

#180507

Purpose

PasCas13b-gRNA targeting EGFP(W58stop) for RNA editing

Depositor

Masaharu Somiya

Article

Somiya et al bioRxiv 2022.01.25.477620

Insert

PasCas13b-gRNA targeting EGFP(W58stop)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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PasCas13b-gRNA-Nluc(W12stop)

Plasmid

#180506

Purpose

PasCas13b-gRNA targeting Nluc(W12stop) for RNA editing

Depositor

Masaharu Somiya

Article

Somiya et al bioRxiv 2022.01.25.477620

Insert

PasCas13b-gRNA targeting Nluc(W12stop)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pT7mitfagRNA

Plasmid

#47931

Purpose

in vitro transcription of mitfa gRNA

Depositor

Wenbiao Chen

Article

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

Insert

mitfa gRNA (mitfa Zebrafish)

Use

CRISPR

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pHEE2E-TRI

Plasmid

#71288

Purpose

Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC

Depositor

Qi-Jun Chen

Article

Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

Inserts

sgRNA targeting TRY and CPC genes
sgRNA targeting ETC2 gene
zCas9

Use

CRISPR; Plant binary vector

Tags

3x FLAG and NLS

Expression

Plant

Mutation

Zea mays codon-optimized Cas9

Promoter

EC1.2 enhancer fused to EC1.1 promoter and U6-26p…

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pJZC43

Plasmid

#66565

Purpose

sgRNA + 2XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 2XPP7
PCP-VP64 IRES mCherry

Expression

Mammalian

Promoter

CMV and U6

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A6-gRNA_(CJT88)

Plasmid

#226987

Purpose

pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A6)

Depositor

Benjamin Kleinstiver

Article

Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

Insert

SpCas9 gRNA A6 to create OPA1-R194G

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A7-gRNA_(CJT89)

Plasmid

#226986

Purpose

pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A7)

Depositor

Benjamin Kleinstiver

Article

Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

Insert

SpCas9 gRNA A7 to create OPA1-R194G

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A9-gRNA_(CJT87)

Plasmid

#226988

Purpose

pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A9)

Depositor

Benjamin Kleinstiver

Article

Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

Insert

SpCas9 gRNA A9 to create OPA1-R194G

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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