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We narrowed to 2,587 results for: pcas

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Showing: 1381 - 1400 of 2587 results

  2. pDG335

    Plasmid
    #100899
    Purpose
    SpCas9n (D10A Nickase mutant) with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100899-plasmid-map-sequence-id-195324

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  7. gCBEv3

    Plasmid
    #220618
    Purpose
    vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv3) driven by EF1a promoter, guide RNAs compatible with SpG driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)
    Promoter
    hU6, EF1a
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220618-plasmid-map-sequence-id-436973

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  11. gTBEv3

    Plasmid
    #220619
    Purpose
    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv3) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    hUNG2-nSpCas9
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…
    Promoter
    hU6, EF1a
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220619-plasmid-map-sequence-id-436981

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  12. pPC1000

    Plasmid
    #174829
    Purpose
    Lentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 prime edit site
    Depositor
    David Liu
    Article
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Inserts
    CRISPRi SpCas9 sgRNA cassette
    PuroR-P2A-BFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    Detailed in manuscript
    Promoter
    EF1a and mouse U6
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174829-plasmid-map-sequence-id-343648

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  13. PX459v3

    Plasmid
    #178799
    Purpose
    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available Since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178799-plasmid-map-sequence-id-354842

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  18. pSLQ10530

    Plasmid
    #239266
    Purpose
    Expresses NES-dCas13b-ABI for CRISPR-TO perturbation in the cytoplasm of cell lines
    Depositor
    Stanley Qi
    Article
    Han et al Nature. 2025 Jul;643(8070):241-251. doi:
    Insert
    NES-dPspCas13b-EGFP-ABI
    Use
    CRISPR and Lentiviral
    Tags
    Flag tag and V5 tag
    Expression
    Mammalian
    Mutation
    H133A and H1058A in PspCas13b
    Promoter
    EF1a
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    239266-plasmid-map-sequence-id-474747

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Showing: 1381 - 1400 of 2587 results