163,403 results

1. pGP-AAV-syn-jGCaMP7b-WPRE (AAV1)

   Viral Prep

   #104489-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pGP-AAV-syn-jGCaMP7b-WPRE (#104489). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7b-WPRE plasmid DNA. Syn-driven jGCaMP7b calcium sensor. jGCaMP7b exhibits the brightest resting fluorescence of the jGCaMP7 variants, and can be used for imaging of small neuronal processes. These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Promoter

   Syn

   Tags

   None

   Available since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

2. CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entry (pRW1509)

   Plasmid

   #225755

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7 in vitro transcription detection

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHR-UCOE-SFFV-Zim3-dCas9-P2A-Hygro

   Plasmid

   #188768

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1-mCherry

   Plasmid

   #128744

   Purpose

   Expresses mCherry in mammalian cells

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRK5-HA-Ubiquitin-WT

   Plasmid

   #17608

   Purpose

   Mammalian expression of HA tagged ubiquitin

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pComb3XTT

   Plasmid

   #63891

   Purpose

   Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)

   Depositor

   Carlos Barbas

   Article

   Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

   Insert

   TT Fab
   

   Use

   Phage display

   Tags

   6xHis, HA tag, and geneIII

   Expression

   Bacterial

   Mutation

   Promoter

   lacZ

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSLCAR-CD19-BBz

   Plasmid

   #135992

   Purpose

   Modular 41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

   Depositor

   Scott McComb

   Article

   Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

   Insert

   FMC63-41BB-3z-P2A-EGFP (CD247, CD28, TNFRSF9 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-short

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 3X ERE TATA luc

   Plasmid

   #11354

   Purpose

   Luciferase reporter containing three copies of vitellogenin Estrogen Response Element

   Depositor

   Donald McDonnell

   Article

   Hall et al Endocrinology. 1999 Dec . 140(12):5566-7

   Insert

   ERE
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFGET19_Ulp1

   Plasmid

   #64697

   Purpose

   H6-Ulp1 from pHYRS52 into pET-28b(+)

   Depositor

   Hideo Iwai

   Article

   Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

   Insert

   ULP1 (ULP1 Budding Yeast, Saccharomyces cerevisiae)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Ulp1(403-621)

   Promoter

   T7

   Available since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMXs-3XHA-EGFP-OMP25

    Plasmid

    #83356

    Purpose

    For tagging mitochondria with HA epitopes (HA-MITO construct)

    Depositor

    David Sabatini

    Article

    Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

    Insert

    3XHA-EGFP-OMP25
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge