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pFastBac Dual PNG172-STII PLU-HIS

Plasmid

#112992

Purpose

Baculovirus expression of Drosophila png172-Flag and plu-His

Depositor

Terry Orr-Weaver

Inserts

png172 (png Fly)
plu (plu Fly)

Tags

Flag and His

Expression

Insect

Mutation

png172 mutation

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac Dual PNG-STII PLU-HIS

Plasmid

#112993

Purpose

Baculovirus expression of Drosophila png-Flag and plu-His

Depositor

Terry Orr-Weaver

Inserts

png (png Fly)
plu (plu Fly)

Tags

Flag and His

Expression

Insect

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBacHT (His10-4xFRB-SNAP-Lck G2A)

Plasmid

#177887

Purpose

Baculo expression of decahistidine tag fused to 4xFRB, 1xSNAP tag and human Lck G2A

Depositor

Ron Vale

Article

Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

Insert

Lck (LCK Human)

Tags

10xHis-TEV-4xFRB-SNAP tag

Expression

Insect

Mutation

G2A

Promoter

Polyhedrin

Availability

Academic Institutions and Nonprofits only

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pre_IV_CS10_1033_v1

Plasmid

#126860

Purpose

4x 1033 b long scaffolds divided by Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 10, made by method IV, yields linear 1033 b long scaffolds after Zn incubation

Depositor

Hendrik Dietz

Article

Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

Insert

IV_CS10_1033_v1

Use

Synthetic Biology

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V3)

Plasmid

#55223

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

SNAPf and TwinStrepII

Expression

Insect

Available Since

July 9, 2014

Availability

Academic Institutions and Nonprofits only

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mycUBB+1

Plasmid

#20254

Depositor

Nico Dantuma

Article

Verhoef et al FASEB J. 2009 Jan . 23(1):123-33.

Insert

Ubiquitin B +1 transcript (UBB Human)

Tags

myc

Expression

Mammalian

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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pre_II_CS3_7746_v3

Plasmid

#126856

Purpose

7653 b scaffold from compatibility group 3, made by method II, containing 2 Zn-dependent self-cleaving DNAzyme cassette for lbisection into 2 3780 b scaffolds

Depositor

Hendrik Dietz

Article

Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

Insert

II_CS3_7746_v3

Use

Synthetic Biology

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac HTB His-TEV-rDPP9-FLAG

Plasmid

#217328

Purpose

Purification of rat DPP9 fusion with C-terminal FLAG tag from insect cells

Depositor

Yifan Wang

Insert

DPP9

Tags

FLAG and His-TEV

Expression

Insect

Promoter

polyhedrin

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac HTB His-TEV-rDPP9(S729A)

Plasmid

#217327

Purpose

Purification of catalytically-dead rat DPP9 from insect cells

Depositor

Yifan Wang

Insert

DPP9

Tags

His-TEV

Expression

Insect

Mutation

S729A

Promoter

polyhedrin

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac HTB His-TEV-BN-rNLRP1-FLAG

Plasmid

#217323

Purpose

Purification of Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cells

Depositor

Yifan Wang

Insert

NLRP1

Tags

FLAG and His-TEV

Expression

Insect

Promoter

polyhedrin

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual-GST-TEV-SINTBAD-mCherry

Plasmid

#208874

Purpose

For expression in insect cells of SINTBAD-mCherry

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

SINTBAD (TBKBP1 Human)

Tags

GST-TEV

Expression

Insect

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac_Dual_GST-TEV-EGFP-TBK1ΔCTD (res 1-666)

Plasmid

#198072

Purpose

Used for the expression and purification of EGFP labelled TBK1 carrying a mutation for the removal of the CTD (SMC Internal No. 1874)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Tags

GST-TEV-EGFP

Expression

Insect

Mutation

TBK1ΔCTD (res 1-666)

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-NFASC-COMP5AP-AviTag-9xHis

Plasmid

#157599

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

NFASC (NFASC Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-FASLG-COMP5AP-AviTag-9xHis

Plasmid

#157143

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

FASLG (FASLG Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLex-FLAG-FASTKD4 mutant delta 226

Plasmid

#73621

Purpose

Expression vector containing FASTKD4 mutant delta 226

Depositor

Vamsi Mootha

Article

Wolf et al Cell Rep. 2014 May 8;7(3):918-31. doi: 1

Insert

FASTKD4 (TBRG4 Human)

Mutation

Y616A, L617A, K618A, K620A

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac Dual PNG172-STII PLU-HIS

Plasmid

#112992

Purpose

Baculovirus expression of Drosophila png172-Flag and plu-His

Depositor

Terry Orr-Weaver

Inserts

png172 (png Fly)
plu (plu Fly)

Tags

Flag and His

Expression

Insect

Mutation

png172 mutation

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac Dual PNG-STII PLU-HIS

Plasmid

#112993

Purpose

Baculovirus expression of Drosophila png-Flag and plu-His

Depositor

Terry Orr-Weaver

Inserts

png (png Fly)
plu (plu Fly)

Tags

Flag and His

Expression

Insect

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBacHT (His10-4xFRB-SNAP-Lck G2A)

Plasmid

#177887

Purpose

Baculo expression of decahistidine tag fused to 4xFRB, 1xSNAP tag and human Lck G2A

Depositor

Ron Vale

Article

Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

Insert

Lck (LCK Human)

Tags

10xHis-TEV-4xFRB-SNAP tag

Expression

Insect

Mutation

G2A

Promoter

Polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V3)

Plasmid

#55223

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

SNAPf and TwinStrepII

Expression

Insect

Available Since

July 9, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
human p53-(1-393)-M340Q-L344R

Plasmid

#24863

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Methionine 340 mutated to Glutamine Leucine 344 …

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only