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Addgene

We narrowed to 5,630 results for: Braf

Showing: 2841 - 2880 of 5630 results
  1. pHSG1C3

    Plasmid
    #164423
    Purpose
    single guide RNA (sgRNA) and Prime editing guide RNA (pegRNA) cloning and mammalian cell expression. BbsI cloning for sgRNAs and BbsI/PstI for pegRNAs.
    Depositor
    Insert
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pMT-ubb-cytoBirA-2a-mCherry

    Plasmid
    #79885
    Purpose
    Ubiquitin promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Ubiquitin
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
  3. pMT-MCS-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80058
    Purpose
    MCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
  4. pMT-flk1-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80056
    Purpose
    Flk1/Kdrl promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    flk1
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
  5. Danio alpha Ba-crystallin 3kb promoter/AcGFP

    Plasmid
    #98097
    Purpose
    aBA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos
    Depositor
    Insert
    Alpha Ba-crystallin promoter 3 kb fragment, Danio rerio (cryaba Zebrafish)
    Use
    Gfp expressing
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Danio alpha Ba-crystallin 3 kb fragment (-3000/-1)
    Available since
    Sept. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
  6. Zed_vector_tol2_-2.1prox1a_2:EGFP_XCA:DsRed2

    Plasmid
    #218208
    Purpose
    Zebrafish -2.1prox1a_2 (not mouse conserved) enhancer reporter in the facial lymphatics at 5 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vector
    Insert
    -2.1prox1a_2
    Use
    Zebrafish expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. pENTR-D-kcnj1b

    Plasmid
    #173126
    Purpose
    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.
    Depositor
    Insert
    kcnj1b (kcnj1b Zebrafish)
    Use
    Pcr cloning vector
    Tags
    Expression
    Mutation
    Promoter
    No promoter at 5 end; T7 promoter at 3 end.
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. pDONR221-kcnj8

    Plasmid
    #173134
    Purpose
    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.
    Depositor
    Insert
    kcnj8 (kcnj8, si:dkey-183c2.2 Zebrafish)
    Use
    Pcr cloning vector
    Tags
    Expression
    Mutation
    Promoter
    No promoter at 5 end; T7 promoter at 3 end.
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 2841 - 2880 of 5630 results