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pTP264

Plasmid

#149334

Purpose

Bacterial expression of Biotin ligase BirA

Depositor

Dirk Görlich

Article

Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

Insert

Biotin ligase BirA

Tags

His14 and bdNEDD8

Expression

Bacterial

Available Since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMC-6-TaGRF-GIF-9

Plasmid

#197747

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

TaGRF-GIF chimeric protein CDS

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMM146

Plasmid

#127218

Purpose

BeYDV replicon with constitutive Luc expression, BBM, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, BBM, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pJL1 BSMT1

Plasmid

#106287

Purpose

Expresses BSMT1 in Ecoli off a T7 promoter

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

BSMT1

Expression

Bacterial

Promoter

T7

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pEE844

Plasmid

#231167

Purpose

T-DNA encoding TRV2 with PaqCI Recognition Sites and Ccdb

Depositor

Daniel Voytas

Article

Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

Type

Empty backbone

Use

Cloning vector

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_WT POLK

Plasmid

#201584

Purpose

Expresses human DNA polymerase kappa with an N-terminal HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

Polymerase kappa (POLK Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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P307-FBP_6

Plasmid

#139697

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs

Use

Luciferase

Expression

Plant

Promoter

various

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pBIG1e

Plasmid

#80615

Purpose

pBIG1e - Step1 vector e (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pLJM60-FLAG NUFIP1

Plasmid

#110950

Purpose

expresses mammalian FLAG-NUFIP1

Depositor

David Sabatini

Article

Wyant et al Science. 2018 May 18;360(6390):751-758.

Insert

NUFIP1 (NUFIP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pSopBC460S

Plasmid

#112514

Purpose

PsopB driven sopB (catalytically inactive) and sigE

Depositor

Olivia Steele-Mortimer

Article

Finn et al PLoS Pathog. 2017 Apr 20;13(4):e1006354.

Insert

sopB (catalytically inactive)/sigE operon

Expression

Bacterial

Mutation

SopB C460S

Promoter

PsopB

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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KillerOrange /pQE-30

Plasmid

#74748

Purpose

KillerOrange gene for bacterial expression

Depositor

Konstantin Lukyanov

Article

Sarkisyan et al PLoS One. 2015 Dec 17;10(12):e0145287. d

Insert

KillerOrange

Tags

HisTag

Expression

Bacterial

Mutation

KillerRed with substitutions G3C Y66W D113S N145S…

Promoter

T5

Available Since

Aug. 11, 2017

Availability

Academic Institutions and Nonprofits only

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FusionRed-pBAD

Plasmid

#54677

Purpose

Localization: Protein Expression Vector , Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Type

Empty backbone

Tags

6xHis and FusionRed

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pMM119

Plasmid

#127213

Purpose

BeYDV replicon with constitutive GFP expression, WUS2, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

GFP, WUS2, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD_A0101

Plasmid

#90998

Purpose

Module A, Promoter: 35S, Gene: AtCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9

Use

CRISPR

Promoter

35S

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBIG1b

Plasmid

#80612

Purpose

pBIG1b - Step1 vector b (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pMOD_A1110

Plasmid

#91031

Purpose

Module A, Promoter: ZmUbi, Gene: TaCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9

Use

CRISPR

Promoter

ZmUbi

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pGWB602-RBCS-tagRFP

Plasmid

#213868

Purpose

Binary vector for the expression of RBCS fused to the tagRFPn plants (for Agrobacterium-mediated genetic transformation.)

Depositor

Yutaka Kodama

Article

Ichikawa et al Plant Physiol. 2023 Oct 4:kiad528. doi:

Insert

RIBULOSE BISPHOSPHATE CARBOXYLASE SMALL CHAIN 1A (RBCS) (RBCS1A Mustard Weed)

Expression

Bacterial and Plant

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 GFP-h-PKD2

Plasmid

#83451

Purpose

GFP-tagged PC2 for Eukaryotic expression (883)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-2 (PKD2 Human)

Tags

GFP

Expression

Mammalian

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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P427-FBP_13

Plasmid

#139704

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtug7, pUBQ1:NhH3H:tNos, p35S:NnHisps:t35S, pAtELF4:NnLuz:trbcse9

Use

Luciferase

Expression

Plant

Promoter

various

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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P336-FBP_11

Plasmid

#139702

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing Luz

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNPGA:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp

Use

Luciferase

Expression

Plant

Promoter

various

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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