We narrowed to 10,006 results for: Uty

1. (829) SB Tet-ON dCasphi-[myc-ZIM3-HA] U6-gStop

   Plasmid

   #195622

   Purpose

   Sleeping Beauty Tet-ON plasmid for induced expression of dCasphi-ZIM3 CRISPRi construct

   Depositor

   Tonia Rex

   Insert

   dCasphi
   

   Use

   Tags

   C-Myc (NLS), HA tag, and ZIM3

   Expression

   Mammalian

   Mutation

   P749A and P753A

   Promoter

   Tight TRE promoter; Tet-ON (doxycycline)

   Available Since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET_RTX

   Plasmid

   #102787

   Purpose

   Expresses the Reverse Transcriptase gene

   Depositor

   Andrew Ellington

   Article

   Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

   Insert

   reverse transcription xenopolymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter + lac operator

   Available Since

   July 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET_RTX_(exo-)

   Plasmid

   #102786

   Purpose

   Expresses the Reverse Transcriptase gene (3'-5' exonuclease deficient mutant)

   Depositor

   Andrew Ellington

   Article

   Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

   Insert

   reverse transcription xenopolymerase with 3'-5' deficient exonuclease
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. FUdeltaGW-rtTA

   Plasmid

   #19780

   Purpose

   3rd generation lentiviral vector

   Depositor

   Konrad Hochedlinger

   Article

   Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

   Insert

   reverse tetracycline transactivator
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 24, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. Fusion of Zika NS2B-NS3 proteins

   Plasmid

   #204815

   Purpose

   Bacterial expression of a fusion of Zika NS2B-NS3 proteins

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Zika NS2B-NS3 fusion (orf1ab Zika)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. M-SPOTIT1.1

   Plasmid

   #171216

   Purpose

   Expresses a mu-opioid receptor-based circular permuted green fluorescent protein sensor

   Depositor

   Wenjing Wang

   Article

   Kroning et al Angew Chem Int Ed Engl. 2021 Mar 4. doi:

   Insert

   M-SPOTIT1.1
   

   Use

   Lentiviral

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. CBF:H2B-Venus

   Plasmid

   #44211

   Depositor

   Anna-Katerina Hadjantonakis

   Article

   Nowotschin et al BMC Dev Biol. 2013 Apr 25;13:15. doi: 10

   Insert

   CBF1-Responsive-Element
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQE 80L Kan T7WT

   Plasmid

   #102790

   Purpose

   Expresses T7 RNA polymerase

   Depositor

   Andrew Ellington

   Article

   Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

   Insert

   T7 RNA polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 3, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. 4xM67 pTATA TK-Luc

   Plasmid

   #8688

   Depositor

   Jim Darnell

   Article

   Besser et al Mol Cell Biol 1999 Feb;19(2):1401-9.

   Insert

   4 repeated M67 sites
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entry (pRW1509)

    Plasmid

    #225755

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7 in vitro transcription detection

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. CROPseq-multi-v2-mTagBFP2-NLS-BsmBI_entry (pRW1506)

    Plasmid

    #225754

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with nuclear-localized mTagBFP2-NLS; v2 is compatible with T7 in vitro transcription detection

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEIAV-SIN6.1 CGFPW

    Plasmid

    #44171

    Depositor

    John Olsen

    Article

    McKay et al Gene Ther. 2006 Apr;13(8):715-24.

    Insert

    enhanced green fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    chromophore mutations

    Promoter

    CMV

    Available Since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+) mCherry

    Plasmid

    #176301

    Purpose

    Expresses mCherry mRNA from CMV promoter

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSCVII-AM

    Plasmid

    #162751

    Purpose

    Retroviral (MSCV) expression of ametrine fluorescent protein

    Depositor

    Maki Nakayama

    Article

    Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

    Insert

    ametrine
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBABE puro H-Ras V12

    Plasmid

    #9051

    Purpose

    Retroviral expression of a constitutively active form of H-Ras

    Depositor

    William Hahn

    Insert

    Hras V12 (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    G12V, constitutively active

    Promoter

    Available Since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. Mito-mEosEM

    Plasmid

    #132706

    Purpose

    application in Correlative light-electron microscopy

    Depositor

    Pingyong Xu

    Article

    Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    mitochondria targeting sequence
    

    Use

    Tags

    mEosEM

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pACYC-Taq

    Plasmid

    #102793

    Purpose

    Expresses Taq DNA polymerase under WT T7 promoter

    Depositor

    Andrew Ellington

    Article

    Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

    Insert

    Taq DNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUA66 theta GFP

    Plasmid

    #105607

    Purpose

    Expresses GFP with no promoter

    Depositor

    Pamela Silver

    Article

    Stirling et al Mol Cell. 2017 Nov 16;68(4):686-697.e3.

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    No Promoter

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. p3E-2a-tdTomato

    Plasmid

    #67707

    Purpose

    3' entry 2A-tdTomato

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Insert

    2A-tdTomato
    

    Use

    Tags

    Expression

    Mutation

    G172S (please see depositor's comments below)

    Promoter

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKJ0463

    Plasmid

    #228715

    Purpose

    Bacterial expression of epT7 RNAP; for protein production and purification

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Jiang et al Science 0, eadr6006

    Insert

    Sumo_epT7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T3M/G47A/E643G

    Promoter

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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