We narrowed to 14,071 results for: cas9

1. pBSKΔB-CBh-Cas9n-pA

   Plasmid

   #82617

   Purpose

   Expresses Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

   Insert

   SpCas9n
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAC1-pCR8-dCas9VP48

   Plasmid

   #48214

   Purpose

   dCas9VP48 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A) fusion with VP48 activation domain
   

   Use

   CRISPR; Gateway donor

   Tags

   HA Tag and VP48

   Mutation

   D10A;H840A

   Available Since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-FLAG-eSpCas9

   Plasmid

   #126769

   Purpose

   Expression of increased fidelity eSpCas9 in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   eSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A

   Promoter

   T7

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. TDK promoter dCas9

   Plasmid

   #113657

   Purpose

   dCas9 expression unit plasmid. The dCas9 expression unit plasmids contain connector ConL1, ConRE, and one dCas9 transcriptional unit.

   Depositor

   Jeffrey Barrick

   Article

   Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

   Insert

   TDK gene promoter and dCas9
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   TDK gene promoter

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-GFAP-spCas9

   Plasmid

   #231403

   Purpose

   Expresses spCas9 from GFAP promoter

   Depositor

   Larry Young, Arjen Boender

   Insert

   GFAP promoter (RIC8B Mouse)
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Mutation

   Changed RSV promoter to hSYN promoter

   Promoter

   GFAP

   Available Since

   Oct. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-MMLVgag-dCas9

   Plasmid

   #241827

   Purpose

   Expresses MMLVgag–dCas9 for producing RENDER-dCas9

   Depositor

   James Nuñez

   Article

   Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

   Insert

   MMLVgag-dCas9
   

   Use

   CRISPR

   Tags

   FLAG, HA and TagBFP

   Expression

   Mammalian

   Available Since

   Oct. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-spCas9

   Plasmid

   #231404

   Purpose

   Expresses spCas9 from hSyn promoter

   Depositor

   Larry Young, Arjen Boender

   Article

   Schimmer et al bioRxiv 2024.07.15.603543; doi: https://

   Insert

   hSYN promoter (RIC8B Human)
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Mutation

   Changed RSV promoter to hSYN promoter

   Promoter

   hSYN

   Available Since

   Oct. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKMW123_SpRY-Cas9_U6-entry

   Plasmid

   #244820

   Purpose

   Mammalian expression of SpRY Cas9 with Golden Gate-compatible sgRNA spacer cassette

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   SpRY Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG-SV40 NLS and Nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

   Promoter

   CAG, U6

   Available Since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKMW121_WT-Cas9_U6-entry

   Plasmid

   #244818

   Purpose

   Mammalian expression of WT Cas9 with Golden Gate-compatible sgRNA spacer cassette

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   SpyCas9 (WT)
   

   Use

   CRISPR

   Tags

   3xFLAG-SV40 NLS and Nucleoplasmin NLS

   Expression

   Mammalian

   Promoter

   CAG, U6

   Available Since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKMW122_SpG-Cas9_U6-entry

    Plasmid

    #244819

    Purpose

    Mammalian expression of SpG Cas9 with Golden Gate-compatible sgRNA spacer cassette

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    SpG Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG-SV40 NLS and Nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

    Promoter

    CAG, U6

    Available Since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28(a)-p2c-Cas9

    Plasmid

    #242759

    Purpose

    Expresses Cas9 nuclease with fused p2c targeting ligand for expression in E. coli

    Depositor

    Jason Rasgon

    Article

    Chaverra-Rodriguez et al Nat Commun. 2018 Aug 1;9(1):3008. doi: 1

    Insert

    P2C-Cas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Sept. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB_p300_core-dCas9-EGFP_hygro

    Plasmid

    #226431

    Purpose

    PiggyBac transposon vector containing a dox inducible p300 core-dCas9 fusion protein and EGFP reporter. Hygromycin selection marker

    Depositor

    Alon Goren

    Inserts

    p300 core-dCas9-EGFP fusion protein
    rtTA3-IRES-Hygro
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    HA tag, P2A-EGFP, and p300 core

    Expression

    Mammalian

    Promoter

    Dox inducible minimal CMV and UbC Promoter

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKDcas9 delta cerulean

    Plasmid

    #230939

    Purpose

    non-targeting Reference Control; expresses mCerulean

    Depositor

    Andrew Griffiths

    Article

    Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Mutation

    additional tetO

    Available Since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKDcas9 delta mcherry

    Plasmid

    #230940

    Purpose

    non-targeting Reference Control; expresses mCherry

    Depositor

    Andrew Griffiths

    Article

    Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Mutation

    additional tetO

    Available Since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKDcas9 fis cerulean

    Plasmid

    #230942

    Purpose

    aTc inducible knockdown of Fis

    Depositor

    Andrew Griffiths

    Article

    Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Mutation

    additional tetO

    Available Since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKDcas9 mcaS cerulean

    Plasmid

    #230944

    Purpose

    aTc inducible knockdown of mcaS

    Depositor

    Andrew Griffiths

    Article

    Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Mutation

    additional tetO

    Available Since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKDcas9 csgD cerulean

    Plasmid

    #230945

    Purpose

    aTc inducible knockdown of CsgD

    Depositor

    Andrew Griffiths

    Article

    Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Mutation

    additional tetO

    Available Since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKDcas9 flhDC cerulean

    Plasmid

    #230946

    Purpose

    aTc inducible knockdown of FlhDC

    Depositor

    Andrew Griffiths

    Article

    Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Mutation

    additional tetO

    Available Since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKDcas9 FliZ cerulean

    Plasmid

    #230947

    Purpose

    aTc inducible knockdown of FliZ

    Depositor

    Andrew Griffiths

    Article

    Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Mutation

    additional tetO

    Available Since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-TET1-dCas9

    Plasmid

    #235599

    Purpose

    Expresses Tet1CD-dCas9

    Depositor

    Ting Zhou

    Article

    Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

    Insert

    TET1-dCas9 (TET1 Human, SpCas9 is from Streptococcus pyogenes)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xNLS and TET1 CD

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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