We narrowed to 15,105 results for: pET

1. DJ-1 TEV site pET15b

   Plasmid

   #60687

   Purpose

   Recombinant protein expression of Park7

   Depositor

   Mark Wilson

   Article

   Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

   Insert

   DJ-1 (PARK7 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   TEV site was inserted (see comments below).

   Promoter

   T7

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

2. Brg1 Del1 pET28a

   Plasmid

   #122115

   Purpose

   Expression Brg1 amino acids 1-381 in bacteria

   Depositor

   Paul Herring

   Article

   Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

   Insert

   Brg1 (SMARCA4 Human)
   

   Tags

   His-T7

   Expression

   Bacterial

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28-SUMO-KaiB

   Plasmid

   #68050

   Purpose

   Strain: AM5116, Used for protein purification

   Depositor

   Susan Golden

   Article

   Kim et al Proc Natl Acad Sci U S A. 2012 Oct 30;10

   Insert

   KaiB
   

   Tags

   6xHis and SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-DDX43-K292A

   Plasmid

   #134575

   Purpose

   Express human DDX43 mutant (K292A, ATPase dead) in E. coli

   Depositor

   Yuliang Wu

   Article

   Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

   Insert

   DDX43-K292A (DDX43 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   full-length (K292A)

   Promoter

   T7

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET51b-NAD-Luc

   Plasmid

   #135627

   Purpose

   Bacterial expression of a biosensor for measuring NAD+ levels at the point of care (V0)

   Depositor

   Kai Johnsson

   Article

   Yu et al Nat Metab. 2019 Dec;1(12):1219-1225. doi

   Insert

   NAD-Luc sensor
   

   Tags

   Hisx10 and Strep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-SUMO-KaiA

   Plasmid

   #68049

   Purpose

   Strain: AM5115, Used for protein purification

   Depositor

   Susan Golden

   Article

   Kim et al Proc Natl Acad Sci U S A. 2012 Oct 30;10

   Insert

   KaiA
   

   Tags

   6xHis and SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-24a-STE2

   Plasmid

   #133430

   Purpose

   Yeast STE2 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.

   Depositor

   Gerhard Wagner

   Insert

   STE2 (STE2 Budding Yeast)
   

   Expression

   Bacterial

   Mutation

   Both native Cys (C59S/C252A) have been mutated. S…

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET15b+PCNA1 P186C

   Plasmid

   #66173

   Purpose

   Expresses the P186C mutant of Sulfolobus solfataricus PCNA1

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

   Insert

   DNA polymerase sliding clamp 1
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   P186C

   Promoter

   T7 promoter

   Available Since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-2HMG-mCherry

   Plasmid

   #112739

   Purpose

   Express 2HMG-mCherry in E. coli

   Depositor

   Kyubong Jo

   Article

   Lee et al RSC Adv, 2016, 6, 46291-46298

   Insert

   2HMG-mCherry
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. CNNM3-CBS-YPet

    Plasmid

    #149686

    Purpose

    For the FRET-based binding assay between human CNNM3 CBS domain and PRL2.

    Depositor

    Motoyuki Hattori

    Article

    Cai et al Sci Rep. 2020 Jul 30;10(1):12879. doi: 1

    Insert

    CNNM3-CBS-YPet (CNNM3 Human, Synthetic)
    

    Tags

    8 histidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-PKM2-G415R

    Plasmid

    #122693

    Purpose

    Express His-tagged PKM2 (G415R) protein in bacteria.

    Depositor

    Wen-Ching Wang

    Article

    Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

    Insert

    Pyruvate kinase M2 isoform (PKM Human)
    

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    G415R

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET32a-Trx-PP-hNF2

    Plasmid

    #107148

    Purpose

    bacterial expression

    Depositor

    Tina Izard

    Article

    Chinthalapudi et al Nat Commun. 2018 Apr 6;9(1):1338. doi: 1

    Insert

    neurofibromin 2 (NF2 Human)
    

    Tags

    (His)6-Thioredoxin

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pet 3d alpha 1 beta 2 CP

    Plasmid

    #13450

    Depositor

    John Cooper

    Article

    Wear et al Curr Biol. 2003 Sep 2. 13(17):1531-7.

    Insert

    capping protein alpha 1 beta 2 (mouse) (Capza1 Mouse)
    

    Expression

    Bacterial

    Mutation

    none

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pET28a-Beclin1 (1-242)

    Plasmid

    #24401

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Beclin1 (BECN1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids 1-242 only.

    Available Since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pET-24a-CXCR4

    Plasmid

    #133431

    Purpose

    Human CXCR4 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.

    Depositor

    Gerhard Wagner

    Insert

    CXCR4 (CXCR4 Human)
    

    Expression

    Bacterial

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28-p53 (1-73) (72P)

    Plasmid

    #62083

    Purpose

    Expression of human p53 (1-73) (72P) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

    Insert

    p53 (TP53 Human)
    

    Tags

    HIs

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-73; 72P

    Promoter

    T7

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pET28b(+)-SPN+IFS

    Plasmid

    #83372

    Purpose

    Expresses SPN and IFS complex in E. coli cell

    Depositor

    SeWon Suh

    Article

    Yoon et al J Synchrotron Radiat. 2013 Nov;20(Pt 6):

    Inserts

    SPN
    IFS
    

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28-MRPP3-His

    Plasmid

    #67866

    Purpose

    bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. His tag

    Depositor

    Walter Rossmanith

    Article

    Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

    Insert

    MRPP3 (KIAA0391 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino acid 46 of recombinant MRPP3 is preceded by…

    Promoter

    T7

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21a- hPTP1e-PDZ2

    Plasmid

    #20377

    Depositor

    Andrew Lee

    Article

    Hu et al Biochemistry. 2003 Dec 2. 42(47):13856-6

    Insert

    hPTP1e PDZ2 (PTPN13 Human)
    

    Expression

    Bacterial

    Available Since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28_6xHis-PARP1delWGR-L713F

    Plasmid

    #173941

    Purpose

    Bacterial expression of PARP1 lacking WGR domain but containing L713F gain-of-function mutation

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delWGR-L713F (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 521-660; L713F, V762A

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pFS_0160-pET30-Fusicatenibacter_saccharivorans_ARRAY2

    Plasmid

    #116953

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with classic acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pET21-pelB-LaG-27

    Plasmid

    #172752

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-27
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. pET20b-GpNLuc-6xHis

    Plasmid

    #135932

    Purpose

    Expresses the GpNLuc LumiFluor in bacterial cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    GpNLuc LumiFluor
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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24. pET3-ARL15-His6

    Plasmid

    #201880

    Purpose

    Used for bacterial expression of human protein ARL15

    Depositor

    Richard Kahn

    Insert

    ARL15 (ARL15 Human)
    

    Tags

    6xHis (GSGSGSHHHHHH)

    Expression

    Bacterial

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. pET28a_6HT-TBP6.7(R49A)

    Plasmid

    #112726

    Purpose

    Arg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R49A)
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R49A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. eGFP-Silicatein-pET28

    Plasmid

    #198012

    Purpose

    C-terminal eGFP fusion to truncated silicatein insert with N- and C-terminal hexahistidine tag for purification

    Depositor

    Bryan Berger

    Article

    Vigil et al Materials Advances 2, 2023

    Insert

    eGFP-silicatein
    

    Tags

    hexahistidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. pETM33_ZYMND11_coiled-coil MYND

    Plasmid

    #178493

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    ZYMND11_coiled-coil MYND (ZMYND11 Human, Synthetic)
    

    Tags

    His-GST

    Expression

    Bacterial

    Promoter

    T7/LacO

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. pET3a-UBE2J1 1-283

    Plasmid

    #185348

    Purpose

    In vitro transcription and translation in bacterial system

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    UBE2J1 (UBE2J1 Human)
    

    Expression

    Bacterial

    Mutation

    Cytoplasmic domain only, aa 1-283

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. pET-PesaR-Clux

    Plasmid

    #47658

    Purpose

    PesaR-C promoter, luxCDABE, and terminator in pET17b(AmpR)

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Synth Biol. 2013 Jul 23.

    Insert

    PesaR-C
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Additional esa box between -35 site and the origi…

    Promoter

    PesaR-C

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

30. pET30a-SRAP-delCys2

    Plasmid

    #84568

    Purpose

    Encodes murine SRAP domain (250aa) of Hmces/Srap1 with deletion of catalytic Cys2

    Depositor

    Douglas Feldman

    Article

    Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

    Insert

    Srap1 (Hmces Mouse)
    

    Tags

    6xHis and S-Tag

    Expression

    Bacterial

    Mutation

    deletion of Cys2

    Promoter

    T7

    Available Since

    Nov. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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