Addgene

We narrowed to 9,689 results for: Ada;

Showing: 1441 - 1460 of 9689 results

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pNIC-ZB-SPRTNwt-FL

Plasmid

#110217

Purpose

Expression in E. coli of full-length wild-type SPRTN protein, with His and ZB tags at N-terminus.

Depositor

Kristijan Ramadan

Article

Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

Insert

SPRTN (SPRTN Human)

Use

Tags

Expression

Bacterial

Mutation

P296L (see depositor comments below)

Promoter

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-FbxO11-ΔFbox-pcDNA3.1-

Plasmid

#52502

Purpose

expresses human FbxO11-ΔFbox in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

FbxO11 (FBXO11 Human)

Use

Tags

FLAG-HA

Expression

Mammalian

Mutation

deletion of Fbox (deleted aa 71-116)

Promoter

CMV

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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SPACE-ΔUGI (pRZ1836)

Plasmid

#140243

Purpose

CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-P2A-EGFP

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

SPACE-ΔUGI

Use

Tags

Expression

Mammalian

Mutation

V82G in TadA*, D10A in Cas9, R187W in pmCDA1

Promoter

CMV

Available since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-FbxO11-Jeff-pcDNA3.1-

Plasmid

#52503

Purpose

expresses human FbxO11-Jeff mutant in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

FbxO11 (FBXO11 Human)

Use

Tags

FLAG-HA

Expression

Mammalian

Mutation

Q491L (Jeff)

Promoter

CMV

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pAdCMV/V5-DEST-Y705F-STAT3-3xFlag

Plasmid

#99261

Purpose

Adenoviral vector encoding Flag-tagged murine STAT3 (Y705F)

Depositor

Alejandro Adam

Article

Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

Insert

Signal transducer and activator of transcription 3 - Y705F (Stat3 Mouse)

Use

Adenoviral

Tags

3x-Flag

Expression

Mutation

Y705F (contains a silent mutation: nucleotide 271…

Promoter

CMV

Available since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S159/181/666A

Plasmid

#105310

Purpose

To study altered downstream functions of phospholylation-site null kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S159/181/666A

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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NST3_pOpOn2.1

Plasmid

#228066

Purpose

Expresses NST3 in different cell types in Arabidopsis thaliana upon induction with dexamethasone.

Depositor

Charles Anderson

Article

Tamadaddi et al Ann Bot. 2024 Aug 30:mcae153. doi: 10.10

Insert

NAC SECONDARY WALL THICKENING PROMOTING FACTOR3, NST3 (NAC012 Mustard Weed)

Use

Dexamethasone inducible vector

Tags

Expression

Plant

Mutation

Promoter

CaMV35S for LhGR and pOp6-minimal CaMV35S for NST3

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5-TEX264-SIM1_mut-cGFP

Plasmid

#220212

Purpose

Mammalian expression of TEX264 with SIM1 motif mutated, fused at C-terminus to GFP

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

p.111LIDLY>AAAAA; c.331C>G,332T>C,334A&g…

Promoter

CMV/TetO

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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His-MBP-IST1 N-terminal domain

Plasmid

#184805

Purpose

Bacterial expression of IST1 N-terminal domain

Depositor

James Hurley

Article

Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

Insert

IST1 (IST1 Human)

Use

Tags

His6-MBP

Expression

Bacterial

Mutation

aa 1-187 only

Promoter

T7

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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His-MBP-CHMP1B, S2C

Plasmid

#184803

Purpose

Bacterial expression of CHMP1B (S2C mutation)

Depositor

James Hurley

Article

Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

Insert

CHMP1B (CHMP1B Human)

Use

Tags

His6-MBP

Expression

Bacterial

Mutation

S2C

Promoter

T7

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S159/181/666E

Plasmid

#105314

Purpose

To study altered downstream functions of phospholylation-site mimetic kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S159/181/666E

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pET-EIN-FTO(32-505)

Plasmid

#186466

Purpose

Expresses human RNA demythelase protein FTO 32-505 amino acid sequence with N-terminal fusion with the N-domain of EI ( e.coli protein)

Depositor

Vincenzo Venditti

Article

Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055

Insert

Fat mass obesity associated protein (FTO Human)

Use

Tags

6 Histidine-EIN (N-terminal domain of E.coli Enzy…

Expression

Bacterial

Mutation

amino acid 32-505

Promoter

T7

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7-P2A-GFP

Plasmid

#222996

Purpose

Mammalian expression of SpCas9 PE7 prime editor with P2A-EGFP marker

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7-P2A-EGFP

Use

Tags

P2A-EGFP, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7-P2A-BSD

Plasmid

#224122

Purpose

Mammalian expression of SpCas9 PE7 prime editor with P2A-BSD marker

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7-P2A-BSD

Use

Tags

P2A-BSD, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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PE7_AAVS1_knockin_HDR_donor

Plasmid

#223001

Purpose

HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Hdr donor

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7-mutant (Q20A_Y23A_Y24F_F35A)

Plasmid

#224123

Purpose

Mammalian expression of SpCas9 PE7 mutant prime editor (Q20A_Y23A_Y24F_F35A)

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7-mutant (Q20A_Y23A_Y24F_F35A)

Use

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Q20A_Y23A_Y24F_F35A in La(SSB)1-194

Promoter

CMV

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pQC MCS IRES Puro

Plasmid

#110343

Purpose

γ-Retroviral transfer vector for cloning and expressing your gene of interest. IRES-driven Puromycin selection.

Depositor

Sandra Martha Gomes Dias

Article

Adamoski et al Nat Struct Mol Biol. 2023 Dec;30(12):190

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pNIC-ZB-SPRTN-E112A-FL

Plasmid

#110218

Purpose

Expression in E. coli of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, with His and ZB tags at N-terminus.

Depositor

Kristijan Ramadan

Article

Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

Insert

SPRTN (SPRTN Human)

Use

Tags

His6 and ZB

Expression

Bacterial

Mutation

E112A, P296L (see depositor comments below)

Promoter

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pW210-pPB-GA-inducible-CRISPRa-sa_dCas9-VPR-hygR

Plasmid

#170808

Purpose

PiggyBac vector to express GA-inducible VPR-Sa_dCas9 with hygromycin resistance

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Inserts

GID1-VPR-IRES-HygR
GAI-tagBFP-Sa_dCas9 (GAI Mustard Weed, S. aureus, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Synonymous mutation in PYL1 to remove XhoI site (…

Promoter

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pQC MCS IRES G418

Plasmid

#110344

Purpose

γ-Retroviral transfer vector for cloning and expressing your gene of interest. IRES-driven Geneticin selection.

Depositor

Sandra Martha Gomes Dias

Article

Adamoski et al Nat Struct Mol Biol. 2023 Dec;30(12):190

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge