We narrowed to 5,707 results for: ER;

1. pFSynW Syt1 D303G IRES mRuby3

   Plasmid

   #170640

   Purpose

   Expression of syt1 in cultured neurons by lentiviral transduction

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D303G

   Promoter

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFSynW Syt1 D365E IRES mRuby3

   Plasmid

   #170641

   Purpose

   Expression of syt1 in cultured neurons by lentiviral transduction

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D365E

   Promoter

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX Syt1 C2AB (96-421) D303G

   Plasmid

   #170646

   Purpose

   Expression of syt1 for purification in E. Coli

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D303G

   Promoter

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEX Syt1 C2AB (96-421) D365E

   Plasmid

   #170649

   Purpose

   Expression of syt1 for purification in E. Coli

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D365E

   Promoter

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX Syt1 C2AB (96-421) I367T

   Plasmid

   #170652

   Purpose

   Expression of syt1 for purification in E. Coli

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   I367T

   Promoter

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMT-Diaphanous-GFP

   Plasmid

   #24287

   Depositor

   Ron Vale

   Article

   Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

   Insert

   Diaphanous (dia Fly)
   

   Use

   Tags

   GFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 10, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pCAG-HASAP

   Plasmid

   #138325

   Purpose

   chemigenetic voltage indicator

   Depositor

   Eric Schreiter

   Article

   Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

   Insert

   HASAP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Cenp-B INCENP GFP

   Plasmid

   #45238

   Purpose

   Target INCENP to centromeres via full length CENP-B

   Depositor

   Michael Lampson

   Article

   Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

   Insert

   Cenp-B, INCENP-Δcen, GFP
   

   Use

   Tags

   GFP and full length CENP-B

   Expression

   Mammalian

   Mutation

   CEN box deleted from INCENP (aa1-46)

   Promoter

   Available since

   Sept. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EcMBP165-cpGFP.PPYF.T203V.pRSET

   Plasmid

   #33373

   Purpose

   a single-wavelength maltose sensor constructed from E. coli MalE and cpGFP

   Depositor

   Loren Looger

   Article

   Marvin et al Proteins. 2011 Nov;79(11):3025-36. doi:

   Insert

   Improved MBP based Maltose Biosensor
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   cpGFP146 variant from GCaMP2 inserted between AA1…

   Promoter

   T7

   Available since

   Dec. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMRX-INU-FLAG-FAM134Bs

    Plasmid

    #128261

    Purpose

    Expresses FAM134Bs in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    FAM134Bs (RETREG1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pU6-cGAS g2 Cas9-T2A-mCherry

    Plasmid

    #164253

    Purpose

    Transcription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    sgcGAS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEM705 Gso (Ser)

    Plasmid

    #109353

    Purpose

    GsX chimera with Gαo C-terminus (Pertussis toxin insensitive mutant)

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gso chimera
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Changed Cysteine at position 391 to Serine (Pertu…

    Promoter

    CAG

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFSW Syt1 WT IRES GFP

    Plasmid

    #133816

    Purpose

    Encodes full-length wild type synaptotagmin 1 for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Expression

    Mutation

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRN3P_TIR1

    Plasmid

    #119766

    Purpose

    in vitro transcription for mRNA

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Borsos et al Nature. 2019 May;569(7758):729-733. doi:

    Insert

    TIR1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FRB-mCherry-EEA1

    Plasmid

    #222419

    Purpose

    To express an early endosome "anchor" for rapamycin-induced heterodimerisation in mammalian cells

    Depositor

    Stephen Royle

    Article

    Downie et al bioRxiv 2024.05.31.596797

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Tags

    FRB-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pU6-cGAS g3 Cas9-T2A-mCherry

    Plasmid

    #164254

    Purpose

    Transcription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    sgcGAS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EGFP-Miro1v4

    Plasmid

    #174111

    Purpose

    Expresses the peroxisomal variant (splice variant 4) of Miro with an N-terminal EGFP.

    Depositor

    Pietro De Camilli

    Article

    Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    Miro1 (var4, peroxisomal splice variant)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LacI INCENP

    Plasmid

    #45236

    Purpose

    Target INCENP to lac operator sequence

    Depositor

    Michael Lampson

    Article

    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

    Insert

    LacI, INCENP-Δcen, mCherry (lacI )
    

    Use

    Tags

    LacI (lac repressor protein) and mCherry

    Expression

    Mammalian

    Mutation

    CEN box deleted from INCENP (aa1-46)

    Promoter

    t7

    Available since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEM705 Gso (Cys)

    Plasmid

    #109375

    Purpose

    GsX chimera with Gαo C-terminus (Pertussis toxin sensitive)

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gso chimera
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Cys at position 391 (Pertussis Toxin Sensitive)

    Promoter

    CAG

    Available since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRN3P_Kdm5b_MUT

    Plasmid

    #119767

    Purpose

    in vitro transcription for mRNA

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Borsos et al Nature. 2019 May;569(7758):729-733. doi:

    Insert

    Kdm5b MUT
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge