We narrowed to 8,931 results for: Pol

1. pGEX6P-1 human HUWE1 WWE (1611-1703)

   Plasmid

   #132614

   Purpose

   expresses human HUWE1 WWE (1611-1703) in E. coli

   Depositor

   Wenqing Xu

   Article

   Wang et al Genes Dev. 2012 Feb 1;26(3):235-40. doi:

   Insert

   HUWE1 WWE (1611-1703)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-hSyn-mCherry-T2A-miniSOG-C-Far

   Plasmid

   #193915

   Purpose

   Expresses miniSOG targeted to the plasma membrane of neurons, joined by a T2A cleavage site with mCherry; for AAV production

   Depositor

   Xiao Wang

   Article

   Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

   Insert

   miniSOG
   

   Use

   AAV

   Tags

   Farnesylation signal sequence and Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. KA501: pMVP (L3-L2) pA; CMV::TETa-pA

   Plasmid

   #121801

   Purpose

   pMVP L3-L2 entry plasmid, contains polyA + CMV-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds CMV-driven TETa downstream of gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   polyA + CMV::TETa-polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. KO701: pMVP (L3-L2) HA tag + pA; EF1a::TETa-pA

   Plasmid

   #121806

   Purpose

   pMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven TETa

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   HA epitope tag-polyA + EF1a::TETa-polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LentiGuide-Puro-CTRLg2 (CG510)

   Plasmid

   #139456

   Purpose

   Lentiviral vector with non-targeting gRNA and puromycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   Non-targeting sgRNA inserted; resistance gene: puroR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a / U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJB50

   Plasmid

   #226311

   Purpose

   E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   CsoS2 with V(T/S)G to AAA CsoS2 MR1-7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…

   Promoter

   pTRC

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJB55

   Plasmid

   #226310

   Purpose

   E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   CsoS2 with V(T/S)G to AAA CsoS2 MR1-5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…

   Promoter

   pTRC

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHis-WzzE

   Plasmid

   #196661

   Purpose

   Expression of N-terminally His6-tagged WzzE from Pectobacterium atrosepticum in E.coli

   Depositor

   James Naismith

   Article

   Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

   Insert

   WzzE
   

   Use

   Tags

   TEV-cleavable His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGST-WzzE

   Plasmid

   #196662

   Purpose

   Expression of N-terminally GST-tagged WzzE from Pectobacterium atrosepticum in E.coli

   Depositor

   James Naismith

   Article

   Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

   Insert

   WzzE
   

   Use

   Tags

   TEV-cleavable GST

   Expression

   Bacterial

   Mutation

   Valine insertion at position 2.

   Promoter

   T7

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHis-mCherry-WzzE

    Plasmid

    #196663

    Purpose

    Expression of N-terminally His6-mCherry-tagged WzzE from Pectobacterium atrosepticum in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    WzzE
    

    Use

    Tags

    His and mCherry

    Expression

    Bacterial

    Mutation

    Valine insertion at position 2 of WzzE.

    Promoter

    T7

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPglL-strep-mCherry

    Plasmid

    #196664

    Purpose

    Expression of C-terminally distrep tag II-mCherry-tagged PglL from Neisseria meningitidis in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    PglL
    

    Use

    Tags

    TEV-cleavable distrep tag II and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPglL-strep-mClover3

    Plasmid

    #196665

    Purpose

    Expression of C-terminally distrep tag II-mClover3-tagged PglL from Neisseria meningitidis in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    PglL
    

    Use

    Tags

    TEV-cleavable di-strep tag II and mClover3

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pWzyE-GST-distrepII

    Plasmid

    #196666

    Purpose

    Expression of C-terminally GST-distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    WzyE
    

    Use

    Tags

    GST and TEV-cleavable di-strep tag II

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pWzyE-strep-mClover3

    Plasmid

    #196668

    Purpose

    Expression of C-terminally distrep tag II-mClover3-tagged WzyE from Pectobacterium atrosepticum in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    WzyE
    

    Use

    Tags

    TEV-cleavable di-strep tag II and mClover3

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pENTR4 Ta-SRO1 JN177

    Plasmid

    #192546

    Purpose

    pENTR plasmid Triticum aestivum SRO1, full CDS, cultivar JN177, no stop codon

    Depositor

    Lennart Wirthmueller

    Article

    Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

    Insert

    SIMILAR TO RCD ONE 1 (SRO1), cultivar Jinan 177 (JN177)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pENTR4 Ta-sro1 SR3

    Plasmid

    #192547

    Purpose

    pENTR plasmid Triticum aestivum sro1, full CDS, cultivar SR3, no stop codon

    Depositor

    Lennart Wirthmueller

    Article

    Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

    Insert

    SIMILAR TO RCD ONE 1 (SRO1), cultivar Shanrong No. 3 (SR3)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. L2_NOP1gRNA-Cas9-CsA

    Plasmid

    #136139

    Purpose

    Plasmid with NOP1-gRNA to tranform Mp as CRISPR control. Contains Cas9 and HygR

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-35Sx2:HygR p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. wtVP1 only

    Plasmid

    #166673

    Purpose

    Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1, with GAL1 promoter. Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Insert

    Wild-type Murine polyomavirus VP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEMS2267

    Plasmid

    #158392

    Purpose

    High Copy number plasmid containing Ple341 (PCP2 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple341 (PCP2 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Ple341

    Available since

    Feb. 4, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pEMS2279

    Plasmid

    #158399

    Purpose

    High Copy number plasmid containing Ple349 (PDE6H MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple349 (PDE6H Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Ple349

    Available since

    Feb. 4, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge