We narrowed to 34,771 results for: cas

1. pCSD_SpCas9MT3-FKBP_SL

   Plasmid

   #107302

   Purpose

   Expresses R1335K mutant SpCas9 fused to FKBP in mammalian cells

   Depositor

   Scot Wolfe

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   FKBP

   Expression

   Mammalian

   Mutation

   R1335K and fused to FKBP

   Promoter

   CMV IE94

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-CMV-TO-Casp3te-cx

   Plasmid

   #116057

   Purpose

   Reporter plasmid for measuring protease activity

   Depositor

   Michael Elowitz

   Article

   Gao et al Science. 2018 Sep 21;361(6408):1252-1258

   Insert

   Casp3te-cx
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. St1Cas9_LMD-9_sgRNA_pUC19

   Plasmid

   #110627

   Purpose

   U6-driven sgRNA expression plasmid for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9).

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Insert

   Empty St1Cas9 LMD-9 sgRNA backbone
   

   Use

   CRISPR and Synthetic Biology

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAtCas9-D10A_1

   Plasmid

   #91159

   Purpose

   Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   AtCas9_D10A (nickase)
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A

   Promoter

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTaCas9-dead_6

   Plasmid

   #91175

   Purpose

   Cas9 only expression, Cas9 Type: TaCas9_D10A+H840A (dead), Plant Selection: PvUbi2:bar

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TaCas9_D10A+H840A (dead)
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A, H840A

   Promoter

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTaCas9-dead_5

   Plasmid

   #91174

   Purpose

   Cas9 only expression, Cas9 Type: TaCas9_D10A+H840A (dead), Plant Selection: PvUbi2:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TaCas9_D10A+H840A (dead)
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A, H840A

   Promoter

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDF-casBCDE(-6)

   Plasmid

   #89730

   Purpose

   Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-6) truncated by 6 nucleotides at the leader-distal end.

   Depositor

   Konstantin Kuznedelov, Konstantin Severinov

   Article

   Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

   Insert

   CRISPR array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Derivative CRISPR array containing a shorter vers…

   Promoter

   Available since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDF-casBCDE(-12)

   Plasmid

   #89731

   Purpose

   Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-12) truncated by 12 nucleotides at the leader-distal end.

   Depositor

   Konstantin Kuznedelov, Konstantin Severinov

   Article

   Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

   Insert

   CRISPR array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Derivative CRISPR array containing a shorter vers…

   Promoter

   Available since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDF-casBCDE(-18)

   Plasmid

   #89732

   Purpose

   Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-18) truncated by 18 nucleotides at the leader-distal end.

   Depositor

   Konstantin Kuznedelov, Konstantin Severinov

   Article

   Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

   Insert

   CRISPR array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Derivative CRISPR array containing a shorter vers…

   Promoter

   Available since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDF-casBCDE(-3)

    Plasmid

    #89729

    Purpose

    Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-3) truncated by 3 nucleotides at the leader-distal end.

    Depositor

    Konstantin Kuznedelov, Konstantin Severinov

    Article

    Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

    Insert

    CRISPR array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Derivative CRISPR array containing a shorter vers…

    Promoter

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. p426_Cas9_gRNA-SAP155c

    Plasmid

    #87390

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting SAP155c sequence ATGAAAGACAACTATAGGGC in yeast chromosome 6

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting SAP155c
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ADH1, pTyrosine

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. p426_Cas9_gRNA-ARS607c

    Plasmid

    #87388

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS607c sequence CTATTTTTGCTTTCTGCACA in yeast chromosome 6.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS607c
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ADH1, pTyrosine

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. p426_Cas9_gRNA-SAP155b

    Plasmid

    #87389

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting SAP155b sequence GGTTTTCATACTGGGGCCGC in yeast chromosome 6

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting SAP155b
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ADH1, pTyrosine

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. p426_Cas9_gRNA-ARS805a

    Plasmid

    #87393

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS805a sequence TTATTTGAATGATATTTAGT in yeast chromosome 8.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS805a
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ADH1, pTyrosine

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. p426_Cas9_gRNA-ARS1206a

    Plasmid

    #87398

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1206a sequence CGAACATTTTTCCATGCGCT in yeast chromosome 12.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1206a
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ADH1, pTyrosine

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. p426_Cas9_gRNA-ARS1414a

    Plasmid

    #87400

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1414a sequence GCGCCACAGTTTCAAGGGTC in yeast chromosome 14.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1414a
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ADH1, pTyrosine

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. p426_Cas9_gRNA-R-ARS805a

    Plasmid

    #87408

    Purpose

    p426_Cas9_gRNA-ARS805a without ribozyme All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS805a sequence TTATTTGAATGATATTTAGT in yeast chromosome 8.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS805a
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ADH1, pTyrosine

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p426_Cas9_gRNA-RDS1a

    Plasmid

    #87385

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting RDS1a sequence ATTCAATACGAAATGTGTGC in yeast chromosome 3

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting RDS1a
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ADH1, pTyrosine

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCASCADE-gapAP1

    Plasmid

    #87146

    Purpose

    gapAP1 targeting guide RNA E. coli , Low Phosphate Induction

    Depositor

    Michael Lynch

    Insert

    gapAP1 guide array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E . coli ugpB gene promoter, low phosphate induct…

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pdCas9-M-4F2

    Plasmid

    #73431

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4F2.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor 4F2 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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