We narrowed to 7,766 results for: loxP

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EF1a_MYCN_P2A_Hygro_Barcode

Plasmid

#120463

Purpose

Barcoded lentiviral vector to express MYCN in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYCN (MYCN Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PBMBP-eGFP

Plasmid

#40976

Depositor

Joseph Loturco

Publication

Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MBP promoter fragment (Mbp Mouse)

Expression

Mammalian

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pU6-sgCD71-2

Plasmid

#46918

Purpose

Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous CD71 gene

Depositor

Stanley Qi, Jonathan Weissman

Publication

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Inserts

sgCD71 -2 (TFRC Human)
Puromycin resistance and mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CMV and U6

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pUA

Plasmid

#58372

Purpose

UAS vector to express gene of interest in Drosophila

Depositor

Chun Han, Yuh-Nung Jan

Publication

Han et al Neuron. 2014 Feb 5;81(3):544-60. doi: 10

Type

Empty backbone

Expression

Insect

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-EWB-DIO-cyan pre-eGRASP(p30)

Plasmid

#111583

Purpose

An AAV vector that expresses double floxed cyan pre-eGRASP(p30) under the Ef1a promoter.

Depositor

Bong-Kiun Kaang

Publication

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

cyan pre-eGRASP(p30)

Use

AAV

Promoter

Ef1a

Available since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3-Dio-GFP

Plasmid

#198279

Purpose

Cre reporter for mammalian cells; double-floxxed-EGFP

Depositor

Feng Zhang

Publication

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Dio-GFP

Expression

Mammalian

Promoter

CMV

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-mRuby2

Plasmid

#104058

Purpose

AAV expression of Cre-dependent reporter mRuby2

Depositor

Viviana Gradinaru

Publication

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

mRuby2

Use

AAV

Promoter

CAG

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pDD268

Plasmid

#132523

Purpose

mNG^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Daniel Dickinson, Bob Goldstein

Publication

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

mNG-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFlag and C. elegans codon-optimized mNG

Expression

Worm

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pSLQ9834_sgTet: pHR-hU6-CasMINI sgRNA_#2 (sgTet); EF1a-Puro-T2A-BFP- WPRE

Plasmid

#176273

Purpose

Expression of CasMINI sgRNA targeting TRE3G promoter in dCasMINI-mediated GFP activation assays.

Depositor

Stanley Qi

Publication

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI sgRNA (sgTet) and BFP

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-R26-FLEX-mStr

Plasmid

#135619

Purpose

Donor vector for genomic targeting of a CAG-FLEX-mStrawberry cassette to the mouse Rosa26 locus

Depositor

Lin He

Publication

Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

Insert

CAG-FLEX-mStrawberry flanked by Rosa26 homology arms (Gt(ROSA)26Sor Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available since

May 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMB-SaCas9

Plasmid

#105998

Purpose

SaCas9 expression vector

Depositor

Mike McManus

Publication

Boettcher et al Nat Biotechnol. 2018 Jan 15. pii: nbt.40

Insert

Cas9 nuclease

Use

CRISPR and Lentiviral

Tags

none

Expression

Mammalian

Mutation

none

Promoter

short EF1alpha

Available since

July 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMK281 (mCherry2-Hygro)

Plasmid

#72797

Purpose

C-terminal tagging with mCherry2 using CRIPSR/Cas9

Depositor

Masato Kanemaki

Publication

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry2-Hygro

Use

CRISPR

Tags

mCherry2

Expression

Mammalian

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pJZC48

Plasmid

#62336

Purpose

sgRNA + 1x COM with COM-VP64 effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 1x COM binding module
COM-VP64

Use

Lentiviral

Tags

VP64

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-EF1α-mKate2-PDE4D3-Cat

Plasmid

#169128

Purpose

AAV plasmid containing a mKate2 and a truncated PDE4D3

Depositor

Georg Nagel

Publication

Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

Has service

AAV1

Insert

mKate2-PDE4DCatL (PDE4D Human)

Use

AAV and Cre/Lox

Tags

mKate2

Expression

Mammalian

Mutation

truncated, Catalatic domain only

Promoter

EF1a

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pPB-CAG-Brainbow3.2-PGK-PuroTK

Plasmid

#159928

Purpose

Brainbow transgenesis

Depositor

Hideyuki Okano

Publication

Yoshimatsu et al Stem Cell Res. 2021 Jan 8;51:102164. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

NFPnls
mOrange2
EGFP
mKate2

Expression

Mammalian

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSMALB-ATF4.12

Plasmid

#155033

Purpose

Fluorescent reporter for ATF4 translation, Negative Control

Depositor

John Dick, Peter van Galen

Publication

van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

Inserts

ATF4 5' UTR (Atf4 Mouse)
ATF4-eGFP fusion ORF
TagBFP

Use

Lentiviral

Expression

Mammalian

Mutation

uORF1 start codon mutated

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCWXPG-UBI-Ngn3PDX1MafA

Plasmid

#114303

Purpose

Constitutive expression of Ngn3, PDX1 and MafA proteins from a precursor with self-cleavable 2A peptides

Depositor

Patrick Salmon

Publication

Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

Insert

Ngn3 (NEUROG3 Human)

Use

Lentiviral

Mutation

Ngn3, PDX1 and MafA proteins are expressed from a…

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pLVE-hTGFB3-IRES-Red

Plasmid

#52580

Purpose

2nd generation lentiviral transfer vector driving expression of human TGF-beta3 and RFP in mammalian cells

Depositor

Charles Gersbach

Publication

Brunger et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

Insert

human transforming growth factor-beta3 (TGFB3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1alpha

Available since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-Ef1a-DIO-VMAT2-WPRE-pA

Plasmid

#39339

Depositor

Bernardo Sabatini

Publication

Tritsch et al Nature. 2012 Oct 11;490(7419):262-6. doi

Insert

Slc18a2 (Slc18a2 Mouse)

Use

AAV; R

Mutation

Myc tag inserted in the first lumenal loop betwee…

Available since

Oct. 9, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pACYC-GST

Plasmid

#62329

Purpose

E. coli pACYC based expression vector with N-terminal TEV cleavable GST-tag

Depositor

Cheryl Arrowsmith

Type

Empty backbone

Tags

Glutathione S-transferase (GST) tag and TEV prote…

Expression

Bacterial

Promoter

T7-lac0

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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