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Plasmid#191007PurposeGateway entry vector with IGFBP2 tagged with CloverDepositorInsertInsulin-Like Growth Factor Binding Protein 2 (IGFBP2 Human)
UseGateway entry vectorTagsCloverExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2337
Plasmid#142037PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF18 (ZNF18 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3163
Plasmid#144639PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPPP1R13B (PPP1R13B Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
PMmEos3.2
Plasmid#203755PurposeEncodes the membrane anchor of Lyn including the first 10 residues of the N terminus, with 1 myristoylation and 1 palmitoylation, with mEos3.2 fused to the C terminus. Used as a fluorescent plasma membrane probe.DepositorInsertLyn n-term (LYN Human)
UseTagsmEos3.2ExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-eYFP-FKBP-MAD1-AA
Plasmid#114038PurposeChemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB)DepositorInsertHuman MAD1 (MAD1L1 Human)
UseTagseYFP-FKBP12ExpressionMammalianMutationMAD2 binding deficient MAD1 K541A/L543APromoterCMVAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_LYN-1/1
Plasmid#91536PurposeProtein expression and purification of human SH3 domain construct LYN-1/1DepositorInsertLYN-1/1 (LYN Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3/TO/V5-DEST-IGFBP2-Clover
Plasmid#191008PurposeLentiviral expression vector for IGFBP2 with a fluorescent Clover tagDepositorInsertInsulin-Like Growth Factor Binding Protein 2 (IGFBP2 Human)
UseLentiviralTagsCloverExpressionMutationPromoterCMVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX Lyn-SH2
Plasmid#46452DepositorInsertLyn (LYN Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 106-213PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-LITAF
Plasmid#178444Purposedoxycycline-inducible expression of Human LITAF in mammalian cellsDepositorInsertLITAF (LITAF Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pECE-M2-BAIAP2 S366A
Plasmid#31657DepositorInsertBAI1-associated protein 2 S366A (BAIAP2 Human)
UseTagsM2ExpressionMammalianMutationchanged Serine 366 to AlaninePromoterAvailable sinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX-BAIAP2 S366A
Plasmid#31676DepositorInsertBAI1-associated protein 2 S366A (BAIAP2 Human)
UseTagsGSTExpressionBacterialMutationchanged Serine 366 to AlaninePromoterAvailable sinceFeb. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
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dCas9-NLS-gs10-cLYN268
Plasmid#188259PurposePlasmid ensures constitutive expression of the C-terminal fragment of split LYN kinase domain (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminusDepositorInsertcLYN268 (LYN Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF2243
Plasmid#141987PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertLITAF (LITAF Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-UNC5B-COMP5AP-AviTag-9xHis
Plasmid#157359PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertUNC5B (UNC5B Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-UNC5B-Fc(DAPA)-AviTag-6xHis
Plasmid#156795PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertUNC5B (UNC5B Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3204
Plasmid#144680PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertLITAF (LITAF Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
LYN gRNA (BRDN0001145823)
Plasmid#77028Purpose3rd generation lentiviral gRNA plasmid targeting human LYNDepositorInsertLYN (LYN Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
LYN gRNA (BRDN0001145318)
Plasmid#77029Purpose3rd generation lentiviral gRNA plasmid targeting human LYNDepositorInsertLYN (LYN Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only