We narrowed to 15,105 results for: pET

1. HOXD9 (human) pET

   Plasmid

   #8570

   Depositor

   Corey Largman

   Insert

   HOXD9 (HOXD9 Human)
   

   Tags

   T7

   Expression

   Bacterial

   Mutation

   PCR human cDNA clone; makes protein by in vitro T…

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-HJURP-CBP

   Plasmid

   #30393

   Depositor

   Yawen Bai

   Article

   Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

   Insert

   HJURP CenpA Binding Domain (CENPA Human, Budding Yeast)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   July 11, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pET20b-delta133HtrA2 A141S

   Plasmid

   #16157

   Depositor

   L. Miguel Martins

   Article

   Strauss et al Hum Mol Genet. 2005 Aug 1. 14(15):2099-1

   Insert

   HtrA2 (HTRA2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   A141S

   Available Since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. CyPet-A206K-PRL2

   Plasmid

   #149685

   Purpose

   For the FRET-based binding assay between human CNNM3 CBS domain and PRL2.

   Depositor

   Motoyuki Hattori

   Article

   Cai et al Sci Rep. 2020 Jul 30;10(1):12879. doi: 1

   Insert

   CyPet-A206K-PRL2
   

   Tags

   8 histidine tag

   Expression

   Bacterial

   Mutation

   CyPet A206K

   Promoter

   T7

   Available Since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28 Set9 mutant

   Plasmid

   #24083

   Depositor

   Danny Reinberg

   Article

   Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

   Insert

   Set9 (SETD7 Human)
   

   Tags

   FLAG and His

   Expression

   Bacterial

   Mutation

   H297A

   Available Since

   Oct. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pET-28a(+)-FPPS

   Plasmid

   #108952

   Purpose

   heterologous expression of FPPS of G. stearothermophilus in E. coli

   Depositor

   Frank Schulz

   Article

   Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

   Insert

   Farnesyldiphosphat synthase (FPPS)
   

   Tags

   His6-Tag

   Expression

   Bacterial

   Available Since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET24a-LaM4-1xTS

   Plasmid

   #162778

   Purpose

   Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to one tyrosine sulfation (TS) motif. LaM4-1xTS also contains a T7, HA, BAP and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

   Insert

   anti-mCherry nanobody fused to a TS site, T7, HA, BAP and His6 epitope
   

   Tags

   BAP, HA, His6, T7, and TS site from proCCK

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21a-mAIM2-deltaPYD

   Plasmid

   #51535

   Purpose

   Express His6-tagged HIN domain of mAIM2 in bacteria

   Depositor

   Emad Alnemri

   Article

   Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

   Insert

   Aim2 (Aim2 Mouse)
   

   Tags

   His6 and T7

   Expression

   Bacterial

   Promoter

   lac operator

   Available Since

   March 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. TrmD_HaeIn/pET28b(+) N,C-tag

   Plasmid

   #12665

   Depositor

   SeWon Suh

   Article

   Kim et al Acta Crystallogr D Biol Crystallogr. 200

   Insert

   tRNA (guanine-N(1)-)-methyltransferase (trmD Haemophilus influenzae)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Jan. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. human TLNRD1 pET151

    Plasmid

    #159384

    Purpose

    Expresses human TLNRD1 in bacterial cells

    Depositor

    Ben Goult

    Article

    Cowell et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    Talin Rod Domain Containing Protein 1 (TLNRD1 Human)
    

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET His10-Src42a

    Plasmid

    #126674

    Purpose

    Expression of His10-Src42a for purification from E. coli

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    Src42a
    

    Expression

    Bacterial

    Available Since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-21a(+)-IS200

    Plasmid

    #12604

    Depositor

    SeWon Suh

    Article

    Lee et al J Biol Chem. 2006 Feb 17. 281(7):4261-6.

    Insert

    IS200 transposase (SSO1474 Sulfolobus solfataricus)
    

    Expression

    Bacterial

    Available Since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET21-pelB-LaG-24

    Plasmid

    #172750

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-24
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21-pelB-LaG-9

    Plasmid

    #172742

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-9
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28-p53 (Mouse 1-87)

    Plasmid

    #62061

    Purpose

    Expression of Mouse p53 (residues 1-87) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

    Insert

    p53 (Trp53 Mouse)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-87

    Promoter

    T7

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

16. C122-E16: CyPet

    Plasmid

    #162896

    Purpose

    Standard Gateway entry clone encoding CyPet fluorescent protein

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    CyPet fluorescent protein
    

    Use

    Synthetic Biology

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. PET28-FnCas12a-EP16

    Plasmid

    #183636

    Purpose

    PET28-FnCas12a-EP16 can efficiently recognize a broad range of PAM sequences including YN (Y = C or T), TAC and CAA.

    Depositor

    Yunzi Luo

    Article

    Wang et al Biotechnol Bioeng. 2019 Jun;116(6):1463-

    Insert

    FnCas12a-EP16
    

    Use

    CRISPR

    Tags

    6×His Tag

    Expression

    Bacterial

    Mutation

    N607R, K613V, N617R, K180S, K660R, D616N

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET20b- hTPI Lys13Arg

    Plasmid

    #50726

    Purpose

    overexpression of the human TPI1 Lys13Arg allele in E.coli

    Depositor

    Markus Ralser

    Article

    Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

    Insert

    Triosephosphate isomerase 1 (TPI1 Human)
    

    Tags

    6xHIS tag

    Expression

    Bacterial

    Mutation

    changed Lysine 13 to Argenine

    Promoter

    T7

    Available Since

    Feb. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-GST-Q11

    Plasmid

    #185790

    Purpose

    Encodes glutathione S-transferase linked to Q11 self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    GST-Q11
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-MBP-creM

    Plasmid

    #112238

    Purpose

    pET28-derived plasmid that expresses an N-terminal maltose binding fusion construct of CreM

    Depositor

    Emily Balskus

    Article

    Waldman et al J Org Chem. 2018 May 29. doi: 10.1021/ac

    Insert

    CreM
    

    Tags

    MBP

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. D5 gene in PETCON vector

    Plasmid

    #87626

    Purpose

    expresses D5 allosteric Zn2+ and fluorescein binding antibody for yeast display

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Protein Sci. 2017 Apr;26(4):807-813. doi

    Insert

    Designed allosteric Zn2+ and fluorescein binding antibody scFv D5
    

    Tags

    3x(G4S) Linker and cMyc tag on backbone

    Expression

    Yeast

    Available Since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. pET21-pelB-LaG-8

    Plasmid

    #172761

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-8
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. pET21-pelB-LaG-5

    Plasmid

    #172760

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-5
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pET30Xa-SBL1(160S)

    Plasmid

    #66872

    Purpose

    Expresses WTsoybean lipoxygenase-1 160S with N-term Histag

    Depositor

    Betty Gaffney

    Article

    Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

    Insert

    soybean lipoxygenase-1 wild type (NEWENTRY Glycine max)
    

    Tags

    Histag from pET30Xa-LIC

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7lac

    Available Since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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25. FKBP-YPet-RanGAP*

    Plasmid

    #175245

    Purpose

    Bacterial expression and purification, SUMOylation substrate that can be recruited to FRB with rapamycin, Ypet is a FRET acceptor for CyPet

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    RANGAP1 (RANGAP1 Human)
    

    Expression

    Bacterial

    Mutation

    Amino acid 562 F to A

    Promoter

    tac

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pET30a BCP1 NTase

    Plasmid

    #195245

    Purpose

    Expresses phage BCP1 NTase

    Depositor

    Douglas Feldman

    Article

    Ho et al Cell Rep. 2023 Mar 22;42(4):112305. doi:

    Insert

    BCP1 phage NTase
    

    Tags

    His6 and SBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. pET30a-Av05 NTase

    Plasmid

    #195243

    Purpose

    Expresses phage Av05 NTase

    Depositor

    Douglas Feldman

    Article

    Ho et al Cell Rep. 2023 Mar 22;42(4):112305. doi:

    Insert

    phage Av05 NTase
    

    Tags

    His6 and SBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. pET28a-BCA-Pep-H

    Plasmid

    #185782

    Purpose

    Encodes enzyme bovine carbonic anhydrase linked to Pep-H self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    BCA-Pep-H
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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29. pBC2T-Ypet-FtnA

    Plasmid

    #108969

    Purpose

    expresses BC2-tag-Ypet-FtnA in mammalian cells

    Depositor

    Ulrich Rothbauer

    Article

    Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

    Insert

    FtnA (ftnA Escherichia coli)
    

    Tags

    BC2-tag and Ypet

    Expression

    Mammalian

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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30. pSB1C3_YPet-PCP (y-plasmid)

    Plasmid

    #127637

    Purpose

    Encodes a fluorecent protein with an RNA binding peptide: YPet-PCP.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme RiboJ and RBS BBa_B0034.

    Depositor

    Friedrich Simmel

    Article

    Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.

    Insert

    YPet
    

    Use

    Synthetic Biology

    Tags

    RNA binding peptide: PP7 coat protein (PCP)

    Expression

    Bacterial

    Promoter

    pJ21306

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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