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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,004 results for: PHI-1;

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Showing: 1461 - 1480 of 3004 results

  5. pSB4A3-PBAD-phzO

    Plasmid
    #165619
    Purpose
    Arabinose-inducible expression of phzO for conversion of phenazine-1-carboxylic acid to 2-hydroxyphenazine
    Depositor
    Martin Buck
    Insert
    phzO
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PBAD
    Available Since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    165619-plasmid-map-sequence-id-331406

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  6. pDmelOR-mRho.V5.mER.hOr47a

    Plasmid
    #126478
    Purpose
    Human codon-optimized D. melanogaster Orco (hOrco) and Or47a (hOr47a). Or47a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells
    Depositor
    Bill Hansson
    Article
    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.
    Inserts
    Or47a (Or47a Fly)
    Orco (Orco Fly)
    Tags
    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens and codon optim…
    Promoter
    CMV
    Available Since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    126478-plasmid-map-sequence-id-246565

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  7. Cav2.2 HA E1658A (EIVA) pcDNA3

    Plasmid
    #206114
    Purpose
    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658A mutation in the P loop of Domain IV which reduces trafficking to the cell surface
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1b (CACNA1B Rabbit)
    Expression
    Mammalian
    Mutation
    E1658A
    Promoter
    CMV
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    206114-plasmid-map-sequence-id-415366

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  8. An R57A, R59A CAAX pMT2

    Plasmid
    #206119
    Purpose
    Cav2.1 N-terminus (amino acids 1-100) with R57A and R59A mutations that prevent dominant-negative suppression of Cav2.1 currents and a C-terminal CAAX (last 10 aa of H.Ras) motif
    Depositor
    Annette Dolphin
    Article
    Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d
    Insert
    cacna1a (Cacna1a Rat)
    Expression
    Mammalian
    Mutation
    R57A, R59A
    Promoter
    Ad MLP/TPL/SV40
    Available Since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    206119-plasmid-map-sequence-id-415935

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  9. An R57A, R59A HA CAAX pMT2

    Plasmid
    #206126
    Purpose
    Cav2.1 N-terminus (amino acids 1-100) with R57A and R59A mutations that prevent dominant-negative suppression of Cav2.1 currents and a C-terminal CAAX (last 10 aa of H.Ras) motif and a HA tag
    Depositor
    Annette Dolphin
    Article
    Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d
    Insert
    cacna1a (Cacna1a Rat)
    Expression
    Mammalian
    Mutation
    R57A, R59A
    Promoter
    Ad MLP/TPL/SV40
    Available Since
    Oct. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    206126-plasmid-map-sequence-id-412911

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  10. Cav2.2 HA EIVA (E1658A) pCAGGS

    Plasmid
    #206082
    Purpose
    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain IV which reduces trafficking to the cell surface
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1b (CACNA1B Rabbit)
    Expression
    Mammalian
    Mutation
    E1658A
    Promoter
    CMV/B-actin
    Available Since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    206082-plasmid-map-sequence-id-411174

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  12. Cav2.2 HA EIVK (E1658K) pcDNA3

    Plasmid
    #206066
    Purpose
    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surface
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1b (CACNA1B Rabbit)
    Expression
    Mammalian
    Mutation
    E1658K
    Promoter
    CMV
    Available Since
    Oct. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    206066-plasmid-map-sequence-id-410269

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  13. Cav2.2 HA EIVK (E1658K) pCAGGS

    Plasmid
    #206096
    Purpose
    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surface
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1b (CACNA1B Rabbit)
    Expression
    Mammalian
    Mutation
    E1658K
    Promoter
    CMV/B-actin
    Available Since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    206096-plasmid-map-sequence-id-406757

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  14. C2 H5 png-Ha/His in Casper

    Plasmid
    #112969
    Purpose
    P element vector for transposon insertion of png-HA/His into Drosophila genome
    Depositor
    Terry Orr-Weaver
    Insert
    png-HA/His (png Fly)
    Use
    P insertion vector
    Tags
    HA/His epitope
    Available Since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112969-plasmid-map-sequence-id-217662

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  16. pT2iU6m5Hoxa13bE4PCh(#765)

    Plasmid
    #184102
    Purpose
    cyclofen-inducible fish Hoxa13b activation via zebrafish transient transgenesis or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    5myc-ZfHoxa13b-ERT2-P2A-mCherry
    Use
    Zebrafish transgenesis (blue eyes) + in vitro tra…
    Tags
    5xMyc
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    Zf-Ubi+SP6
    Available Since
    July 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184102-plasmid-map-sequence-id-365950

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  17. pT22cUm5En2E4P3Che(#1188)

    Plasmid
    #184094
    Purpose
    cyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Insert
    5myc-chkEn2-ERT2-5'fP2A-mCherry
    Use
    Zebrafish transgenesis (blue eyes)
    Tags
    5xMyc
    Mutation
    5'fP2A: GSG-P2A; ERT2: G400V, M543A, L544A, …
    Promoter
    Zf-Ubi
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184094-plasmid-map-sequence-id-363967

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  18. pT22i5uasm5En2E4P3Che(#1208)

    Plasmid
    #184096
    Purpose
    cyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Insert
    5myc-chkEn2-ERT2-5'fP2A-mCherry
    Use
    Zebrafish transgenesis (blue eyes)
    Tags
    5xMyc
    Mutation
    5'fP2A: GSG-P2A; ERT2: G400V, M543A, L544A, …
    Promoter
    5xUAS
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184096-plasmid-map-sequence-id-363966

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  19. S1PR1-DuET

    Plasmid
    #213374
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    S1PR1 (S1PR1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213374-plasmid-map-sequence-id-430730

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  20. GFP Cav2.2 human HAgHA -minus exon 18a -LONG-Cterm in pcDNA3

    Plasmid
    #198914
    Purpose
    N-terminal GFP tagged, exofacial HAgHA tag in Domain II, does not contain exon 18a - Long form of alternatively spliced C-teminus, in pcDNA3 vector
    Depositor
    Annette Dolphin
    Article
    Dahimene et al Function (Oxf). 2023 Oct 19;5(1):zqad060
    Insert
    Cav2.2 (CACNA1B Human)
    Tags
    N-terminal GFP, internal double HA
    Expression
    Mammalian
    Mutation
    exon 18a deleted, exon 19 starts 3 nucleotides ea…
    Promoter
    CMV
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    198914-plasmid-map-sequence-id-392117

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Showing: 1461 - 1480 of 3004 results