We narrowed to 2,341 results for: crispr system

1. pJEP307-pAAV-EFS(No AgeI)-MCS3-pA

   Plasmid

   #113684

   Purpose

   EFS driven Multi Cloning Site-3 without the AgeI restriction enzyme site.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   N/A
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Cytomegalo Virus(CMV)

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tol2-LyzC-RacFRET-U6ac-rac2 guides

   Plasmid

   #168248

   Purpose

   "label active Rac under Rac2 neutrophil-specific knockout background"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   RacFRET
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   LyzC

   Available since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Tol2-LyzC-PBD-GFP-U6ac-rac2 guides

   Plasmid

   #168251

   Purpose

   "label active Rac under Rac2 neutrophil-specific knockout background"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   PBD GFP
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   LyzC

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Tol2-LyzC-Utrophin-GFP-U6ac-Rac2 guides

   Plasmid

   #168254

   Purpose

   "label stable F-actin under Rac2 neutrophil-specific knockout background"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   Utrophin-GFP
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   LyzC

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Tol2-LyzC-PBD-GFP-U6ac-ctrl guides

   Plasmid

   #168252

   Purpose

   "label active Rac; control for neutrophil-specific knockout"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   PBD GFP
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   LyzC

   Available since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Tol2-LyzC-Utrophin-GFP-U6ac-ctrl guides

   Plasmid

   #168253

   Purpose

   "label stable F-actin; control for neutrophil-specific knockout"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   Utrophin-GFP
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   LyzC

   Available since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LLP185 pLVP-dCas9-DNMT3a V2

   Plasmid

   #100936

   Purpose

   dCas9 and DNMT WT on C terminus, 4 NLS, driven by pGK promoter, with P2A site and PURO gene, within a lentiviral transfer backbone

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   dCas9, DNMT3a catalytic domain (DNMT3A S.pyogenes, Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and 3xTy1

   Expression

   Mammalian

   Mutation

   D10A, H840A for dCas9

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. LLP249 pGK-dCas9-DNMT3a V3

   Plasmid

   #100938

   Purpose

   dCas9 and DNMT WT driven by pGK promoter and Puromycin driven by EF1a

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   dCas9, DNMT3a catalytic domain (DNMT3A S.pyogenes, Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and 3xTy1

   Expression

   Mammalian

   Mutation

   D10A, H840A for dCas9

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. sgCRY1

   Plasmid

   #176511

   Purpose

   Expresses sgRNA in mammalian cells

   Depositor

   Han Kyoung Choe

   Article

   Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

   Insert

   hU6-sgCRY1-2 (Cry1, Cry2 Mouse)
   

   Use

   AAV

   Tags

   mCherry (K14E, N210D, E249G)

   Expression

   Mutation

   Promoter

   U6, hSyn

   Available since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-2/3-3'

    Plasmid

    #117325

    Purpose

    CRISPR-Cas9 for miR-124-2/3, 3'

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    gRNA miR-124-2/3-3' (MIR124-3 Synthetic)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-1/2-5'

    Plasmid

    #117322

    Purpose

    CRISPR-Cas9 for miR-124-1/2, 5'

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    gRNA miR-124-1/2-5' (MIR124-1 Synthetic)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYTK036

    Plasmid

    #65143

    Purpose

    Encodes Cas9 as a Type 3 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNHEJ-recX-sacB

    Plasmid

    #158718

    Purpose

    CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recX.

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    recX
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pmyc

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCgCas9_recT

    Plasmid

    #153337

    Purpose

    Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; Kanr

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCas34

    Plasmid

    #82386

    Purpose

    sgRNA targeting YFP expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PEZ3

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Nanog-2A-mCherry

    Plasmid

    #48680

    Purpose

    For knock-in donor vector by CRISPR/CAS system

    Depositor

    Rudolf Jaenisch

    Article

    Yang et al Cell. 2013 Aug 27. pii: S0092-8674(13)01

    Insert

    Nanog (Nanog Mouse)
    

    Use

    CRISPR

    Tags

    Cherry

    Expression

    Mutation

    Promoter

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCfB6364

    Plasmid

    #106151

    Purpose

    EasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying D-serine marker, integration into Yarrowia lipolytica ku70 locus, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNHEJ-recAmu-sacB

    Plasmid

    #158717

    Purpose

    CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recAR61C allele.

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    recA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    R61C

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCfB6627

    Plasmid

    #106159

    Purpose

    EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6682 into Yarrowia lipolytica chromosomal location IntC_2, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Insert

    unknown
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCas59

    Plasmid

    #82396

    Purpose

    sgRNA targeting YFP +787 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PEZ3

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge