161,377 results

1. pKG123

   Plasmid

   #63074

   Purpose

   Retroviral expression of mCherry-TEV-S-tag CENP-C for protein localization and affinity purification (LAP)

   Depositor

   Iain Cheeseman

   Insert

   CENP-C (CENPC Human)
   

   Use

   Retroviral

   Tags

   TEV-S and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV5 HA-TGFBRII

   Plasmid

   #19158

   Depositor

   Joan Massague

   Insert

   Transforming growth factor, beta receptor II (TGFBR2 Human)
   

   Use

   Tags

   HA (YDVPDYASL)

   Expression

   Mammalian

   Mutation

   PCR based mutagenesis

   Promoter

   Available since

   Oct. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. TL026_AAV_hSyn:SEP-GluA1

   Plasmid

   #192519

   Purpose

   Expression of AMPA GluA1 with N terminal super ecliptic phluorin (SEP) fusion

   Depositor

   Adam Cohen

   Article

   Kim et al bioRxiv. 2023 May 29:2023.05.26.541296.

   Insert

   SEP-GluA1
   

   Use

   AAV and Cre/Lox

   Tags

   super ecliptic phluorin

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pg-HIF-1alpha-EGFP

   Plasmid

   #87204

   Purpose

   C-terminally EGFP-tagged human HIF-1a

   Depositor

   Violaine Sée

   Article

   Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

   Insert

   HIF-1a (HIF1A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   L40F, M88V, Q814R

   Promoter

   CMV

   Available since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-GFP11-His3MX

   Plasmid

   #91951

   Purpose

   Universal C-terminal GFP11 tagging

   Depositor

   Rong Li

   Article

   Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA

   Plasmid

   #61592

   Purpose

   A human codon-optimized Cas9 from Staphylococcus aureus (SaCas9).

   Depositor

   Feng Zhang

   Article

   Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

   Insert

   hSaCas9
   

   Use

   AAV and CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28 MBP_PrA/G-H6

   Plasmid

   #176077

   Purpose

   Express His6 tagged MBP_PrA/G in Escherichia coli

   Depositor

   Tom Rapoport

   Article

   Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

   Insert

   MBP PrA/G-H6
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pD649-HAsp-LAIR1-COMP5AP-AviTag-9xHis

   Plasmid

   #157101

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   LAIR1 (LAIR1 Human)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEIAV-SIN6.1 CGFPW

   Plasmid

   #44171

   Depositor

   John Olsen

   Article

   McKay et al Gene Ther. 2006 Apr;13(8):715-24.

   Insert

   enhanced green fluorescent protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   chromophore mutations

   Promoter

   CMV

   Available since

   May 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCEP4-myc-HLA-B7

    Plasmid

    #135509

    Purpose

    Mammalian expression of myc-tagged HLA-B*07:02

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-B*07:02 (HLA-B Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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