164,599 results

1. pBSV2G_PflaB-msfGFPBb

   Plasmid

   #118231

   Purpose

   Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfGFP

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

   Insert

   flagellin promoter driven msfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAGM4723

   Plasmid

   #48015

   Purpose

   Please see depositor comments section.

   Depositor

   Sylvestre Marillonnet

   Article

   Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

   Type

   Empty backbone

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 3, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pCMV-hAR

   Plasmid

   #89078

   Purpose

   mammalian expression of human androgen receptor in pCMV5

   Depositor

   Elizabeth Wilson

   Article

   Lubahn et al Science. 1988 Apr 15;240(4850):327-30.

   Insert

   human androgen receptor (hAR) full-length (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. peSCBE3-NG-Hypa

   Plasmid

   #218165

   Purpose

   This plasmid harbors the base editor eSCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   escbe3-NG-Hypa
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Tags

   Expression

   Bacterial

   Mutation

   Mutation in the portion of deaminase hAPOBEC3A : …

   Promoter

   rpsL promoter

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTRANS_220

   Plasmid

   #91113

   Purpose

   Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: none

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA-HIF1alpha P402A/P564A-pcDNA3

   Plasmid

   #18955

   Depositor

   William Kaelin

   Article

   Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

   Insert

   Hypoxia inducible factor 1 alpha (HIF1A Human)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   cDNA changed amino acids: Proline 402 to Alanin…

   Promoter

   Available since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBAD-I-SceI

   Plasmid

   #60960

   Purpose

   Plasmid that allows an arabinose-inducible expression of the I-SceI homing endonuclease - for the en passant (two-step Red-mediated) mutagenesis

   Depositor

   Nikolaus Osterrieder

   Article

   Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

   Insert

   I-SceI restriction enzyme
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. Tet-off Full length Tau (0N4R, P301L/V337M)

   Plasmid

   #188573

   Purpose

   Dox-repressible expression of the 0N4R Tau isoform

   Depositor

   Franz-Ulrich Hartl

   Article

   Saha et al Nat Commun. 2023 Feb 2;14(1):560. doi: 1

   Insert

   0N4R Tau (MAPT Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   P301L and V337M

   Promoter

   pTight TRE

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSEED-sfGFP

   Plasmid

   #219639

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   sfGFP SEED Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5/FRT/TO_IgKchL_HA_HaloTag9_myc_PDGFRtmb

    Plasmid

    #175544

    Purpose

    Expression of HaloTag9 at the outer leaflet of the plasma membrane of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    IgKchL_HA-HaloTag9-myc_PDGFRtmb
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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