169,383 results

1. OpenCRISPR-1 sgRNA-12nt stem

   Plasmid

   #221567

   Purpose

   Express OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

   Depositor

   Ali Madani

   Article

   Ruffolo et al bioRxiv 2024.04.22.590591

   Insert

   OpenCRISPR-1 sgRNA 12nt stem loop
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. hCRISPRi-v2, Membrane Proteins (h6), top 5 sgRNAs/gene

   Pooled Library

   #83976

   Purpose

   Human CRISPRi Pooled Library targeting membrane proteins

   Depositor

   Jonathan Weissman

   Article

   Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

   Available since

   Dec. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3-FLAG-CRBN-pGK-HYG

   Plasmid

   #107380

   Purpose

   Mammalian expression of FLAG-CRBN

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   cereblon (CRBN Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPROEX Aqua

   Plasmid

   #42889

   Depositor

   Fabienne Merola

   Article

   Erard et al Mol Biosyst. 2013 Feb 2;9(2):258-67. doi

   Insert

   Aquamarine
   

   Use

   Tags

   6xHis and TEV cleavage

   Expression

   Bacterial

   Mutation

   T65S and H148G

   Promoter

   Available since

   March 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-Neo-Bam APC 1-1941

   Plasmid

   #16510

   Depositor

   Bert Vogelstein

   Article

   Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

   Insert

   APC (APC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Codon 1941 truncation

   Promoter

   Available since

   April 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-Neo-Bam APC 1-1941

   Plasmid

   #16510

   Depositor

   Bert Vogelstein

   Article

   Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

   Insert

   APC (APC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Codon 1941 truncation

   Promoter

   Available since

   April 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pN1-CMV-mCherryN-IntN-DrBphP (N23)

   Plasmid

   #210724

   Purpose

   NIR optogenetic module for conditional protein splicing of split mCherry (N-part)

   Depositor

   Vladislav Verkhusha

   Article

   Karasev et al J Mol Biol. 2023 Dec 15;435(24):168360.

   Insert

   N23
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAS

   Plasmid

   #60847

   Purpose

   Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast

   Depositor

   Jamie Cate

   Article

   Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife

   Inserts

   S. pyogenese Cas9
   RNA pol III promoter (tRNA-Tyr)
   hepatitis delta virus ribozyme, genomic
   sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   NLS/His8 and TTT 3' extension prior to sgRNA

   Expression

   Bacterial and Yeast

   Mutation

   L4 is UUCG tetraloop and guide targets LYP1 (CATA…

   Promoter

   Available since

   Jan. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-BI

   Plasmid

   #126475

   Purpose

   High-copy number vector for the co-expression of two genes in mammalian cells

   Depositor

   Bill Hansson

   Article

   Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET His6 GFP TEV LIC cloning vector (1GFP)

    Plasmid

    #29663

    Depositor

    Scott Gradia

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    6xHis, GFP, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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