We narrowed to 43,126 results for: cha

1. pMB34

   Plasmid

   #122245

   Purpose

   Lentivirus delivery for stable expression of LbCas12a-VPR

   Depositor

   Darjus Tschaharganeh

   Article

   Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

   Insert

   mammalian codon-optimized Lachnospiraceae bacterium Cas12a fused to VPR activator
   

   Use

   Lentiviral

   Tags

   NLS and VPR activator

   Expression

   Mutation

   Promoter

   EFs

   Available since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. HcKCR2_mCherry_pcDNA3.1

   Plasmid

   #177337

   Purpose

   Expresses KCR2 in mammalian cells

   Depositor

   John Spudich

   Article

   Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

   Insert

   HcKCR2
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   mammalian codon-adapted

   Promoter

   Available since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pScPPX2

   Plasmid

   #112877

   Purpose

   Expresses His-tagged yeast exopolyphosphatase for purification from E. coli

   Depositor

   Michael Gray, Ursula Jakob

   Article

   Gray et al Mol Cell. 2014 Mar 6;53(5):689-99. doi:

   Insert

   PPX1 (PPX1 Budding Yeast)
   

   Use

   Tags

   6X His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PB_TRE_sg30-PDGFR_EF1a_mCherry

   Plasmid

   #186309

   Purpose

   Plasmid for inducible mammalian cell expression of the sg30 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   sg30-PDGFR
   mCherry
   

   Use

   Tags

   His, myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCR1068

   Plasmid

   #111092

   Purpose

   BFP/puromycin marked gRNA vector. Contains sgRNA against GFP/BFP N-terminus.

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   BlpI site disrupted

   Promoter

   Available since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PB_TRE_Z18-PDGFR_EF1a_eGFP

   Plasmid

   #186304

   Purpose

   Plasmid for inducible mammalian cell expression of the Z18 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction.

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   Z18-PDGFR
   eGFP
   

   Use

   Tags

   HA, myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG-hChR2(H134R)-EYFP

   Plasmid

   #114367

   Purpose

   expresses channelrhodopsin 2 fused with EYFP

   Depositor

   Mingshan Xue

   Article

   Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

   Insert

   hChR2(H134R)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   the Histidine at amino acid position 134 was chan…

   Promoter

   pCAG

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAct:dCas9-GCN4

   Plasmid

   #78899

   Purpose

   Expresses dCas9-GCN4 under actin promoter for SunTag CRISPRa in Drosophila cell culture

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   dCas9-10XGCN4
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Insect

   Mutation

   Promoter

   pActin (Drosophila)

   Available since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTi4.0_SpdCas9_pmcDA1

   Plasmid

   #204613

   Purpose

   CRISPR cytosine deaminase integrative plasmid for Mycoplasma gallisepticum

   Depositor

   Pascal Sirand-Pugnet

   Article

   Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mRIP3 GFP wt

    Plasmid

    #41382

    Depositor

    Francis Chan

    Article

    Cho et al Cell. 2009 Jun 12;137(6):1112-23.

    Insert

    RIP3 (Ripk3 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-myc-Ataxin3Q84

    Plasmid

    #22124

    Depositor

    Henry Paulson

    Article

    Chai et al J Neurosci. 1999 Dec 1. 19(23):10338-47.

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

12. ZJOM160

    Plasmid

    #133670

    Purpose

    Integration of mTagBFP2-SKL as an additional copy in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast peroxisome.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    pTPI1-mTagBFP2-SKL
    

    Use

    Tags

    mTagBFP2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAct:dCas9

    Plasmid

    #78903

    Purpose

    Expresses dCas9 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    pActin (Drosophila)

    Available since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ZJOM27

    Plasmid

    #133641

    Purpose

    Integration of GFP-PHO8 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast vacuole.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    pCUP1-GFP-Pho8 (PHO8 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ZJOM144

    Plasmid

    #133630

    Purpose

    Integration of SS-GFP-HDEL in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae) Marker for yeast endoplasmic reticulum (ER).

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    pTPI1-SS-GFP-HDEL
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. hRIP1 HA GFP K45A

    Plasmid

    #41389

    Depositor

    Francis Chan

    Article

    Cho et al Cell. 2009 Jun 12;137(6):1112-23.

    Insert

    RIP1 (RIPK1 Human)
    

    Use

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    Kinase Domain Mutant (K45A)

    Promoter

    CMV

    Available since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

17. CaM/pIRES2-eGFP

    Plasmid

    #111499

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    calmodulin [Xenopus laevis] (calm2 Frog)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PB_TRE_Z17-PDGFR_EF1a_mCherry

    Plasmid

    #186303

    Purpose

    Plasmid for inducible mammalian cell expression of the Z17 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction.

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    Z17-PDGFR
    mCherry
    

    Use

    Tags

    HA, myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHRdSV40_scFv_GCN4_sfGFP-VPR-GB1_NLS

    Plasmid

    #79373

    Purpose

    Recruits VPR to a compatible Cas9 protein for transcriptional activation

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    GCN4-sfGFP-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHRdSV40_scFv_GCN4_sfGFP_p65-hsf1_GB1_NLS

    Plasmid

    #79372

    Purpose

    Recruits p65-hsf1 to a compatible Cas9 protein for transcriptional activation

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    GCN4-sfGFP-p65-hsf1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMT85_SpdCas9_pmcDA1

    Plasmid

    #204614

    Purpose

    CRISPR cytosine deaminase integrative plasmid for Mycoplasma bovis

    Depositor

    Pascal Sirand-Pugnet

    Article

    Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. KG#121

    Plasmid

    #110879

    Purpose

    Expresses the C. elegans unc-31 cDNA pan-neuronally

    Depositor

    Kenneth Miller

    Article

    Charlie et al Genetics. 2006 Feb;172(2):943-61. doi: 1

    Inserts

    rab-3 promoter
    unc-31 cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Missing first 408bp

    Promoter

    Available since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMB22

    Plasmid

    #122244

    Purpose

    Lentivirus delivery for stable expression of AsCas12a-VPR

    Depositor

    Darjus Tschaharganeh

    Article

    Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

    Insert

    mammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator
    

    Use

    Lentiviral

    Tags

    NLS and VPR activator

    Expression

    Mutation

    Promoter

    EFs

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMRMTK-clo6

    Plasmid

    #203957

    Purpose

    Cloning plasmid containing the kanamycin resistance gene insert

    Depositor

    Nathan Crook

    Article

    van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

    Insert

    Kanamycin Resistance Gene
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAct:dCas9-VP64

    Plasmid

    #78901

    Purpose

    Expresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    dCas9-VP64
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    pActin (Drosophila)

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMRMTK-clo10

    Plasmid

    #203961

    Purpose

    Cloning plasmid containing the erythromycin resistance gene insert

    Depositor

    Nathan Crook

    Article

    van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

    Insert

    Erythromycin Resistance Gene
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    N/A

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pNC1011

    Plasmid

    #42898

    Depositor

    Beverly Errede

    Article

    Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

    Insert

    3 tandem copies of GFP*
    

    Use

    Yeast integrating

    Tags

    Expression

    Mutation

    changed Phenylalanine 64 to Leucine, Serine 65 t…

    Promoter

    None

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. U6-spTRE3G-CMV-mTagBFP2

    Plasmid

    #102854

    Purpose

    A plasmid encoding TRE3G sgRNA (for SpCas9) and mTagBFP

    Depositor

    Michael Lin

    Article

    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

    Insert

    sp-TRE3G-sgRNA; mTagBFP2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PB_TRE_AP4-PDGFR_EF1a_eBFP2

    Plasmid

    #186306

    Purpose

    Plasmid for inducible mammalian cell expression of the AP4 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    AP4-PDGFR
    eBFP2
    

    Use

    Tags

    HA, myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET-sCS4-2T

    Plasmid

    #28250

    Depositor

    Yawen Bai

    Article

    Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

    Insert

    single chain Cse4-Scm3-H4 with 2 Thrombin site
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    A thrombin cutting site between Cse4 and Scm3. An…

    Promoter

    Available since

    July 12, 2011

    Availability

    Academic Institutions and Nonprofits only

31. pCfB8792

    Plasmid

    #126910

    Purpose

    Template for PCR amplification of ADE2 gRNA biobrick for targeting Saccharomyces cerevisiae ADE2 gene

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    ADE2 gRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pES.HSV2.3a

    Plasmid

    #69593

    Purpose

    TCR a chain construct for generation of gBT-I mice

    Depositor

    Francis R. Carbone

    Article

    Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

    Insert

    TCR alpha chain cDNA from clone HSV2.3 (Tcra Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H-2Kb

    Available since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

33. pCfB5191

    Plasmid

    #126911

    Purpose

    Plasmid containing gRNA expression cassettes targeting the X-4 and XII-5 integration sites in Saccharomyces cerevisiae

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    X-4 and XII-5 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET/LEGH

    Plasmid

    #108226

    Purpose

    Bacterial expression of EGFP with N-terminal lanthanide binding tag and C-terminal His6 tag

    Depositor

    Didier Picard

    Article

    Bhattacharya et al Sci Rep. 2018 Feb 12;8(1):2801. doi: 10.

    Insert

    LBT-EGFP-His6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7, lacO

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pNM3749

    Plasmid

    #154108

    Purpose

    loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Inserts

    LoxP-SEC-LoxP
    FRT::rpl-28p::GFP::his-58::FRT3
    

    Use

    Cre/Lox; Rmce

    Tags

    Expression

    Worm

    Mutation

    Promoter

    not applicable

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pKH-MoHyPer

    Plasmid

    #78267

    Purpose

    Express HyPer-AS sensor that change excitation wavelength with the presence of hydrogen peroxide.

    Depositor

    Nicole Donofrio, Jeffrey Caplan

    Article

    Huang et al Mol Plant Pathol. 2016 Mar 7. doi: 10.11

    Insert

    MoHyper
    

    Use

    Express in magnaporthe

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Rp27

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

37. pCASP-HilA empty vector

    Plasmid

    #153333

    Purpose

    Triggers HilA overexpression from Salmonella upon induction with arabinose without any synthetic protein expressed

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Type

    Empty backbone

    Use

    Salmonella expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. bactin:GFP-DCX

    Plasmid

    #105961

    Purpose

    transgenesis, ubiquitous expression

    Depositor

    Caren Norden

    Article

    Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

    Insert

    DCX (DCX Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pASH3 [pmyo-3::BeCNG1-YFP]

    Plasmid

    #168167

    Purpose

    Expression of BeCNG1-YFP in BWMs of C. elegans

    Depositor

    Alexander Gottschalk

    Article

    Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

    Insert

    BeCNG1-YFP
    

    Use

    Tags

    YFP

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAct:dCas9-scFv

    Plasmid

    #78900

    Purpose

    Expresses scFv-VP64 under actin promoter for SunTag CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    scFV-VP64
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    pActin (Drosophila)

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge