We narrowed to 8,896 results for: Plat

1. His-DERA

   Plasmid

   #203225

   Purpose

   For protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

   Depositor

   Vern Schramm

   Article

   Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

   Insert

   deoxyribose aldolase
   

   Use

   Tags

   N-terminal TEV cleavable 6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMaCTag-P27

   Plasmid

   #120038

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 1xHA

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   1xHA
   

   Use

   Pcr template

   Tags

   1xHA

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBbdCas9S_P0526-sgRNAmreB

   Plasmid

   #149654

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi mreB

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNAmreB
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30, P0526

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. CCR5-donor-MCS

   Plasmid

   #192269

   Purpose

   Donor template for CRISPR Genome Editing

   Depositor

   Shantanu Chowdhury

   Article

   Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

   Insert

   MCS within donor template
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMaCTag-P26

   Plasmid

   #120037

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mAID-Clover

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mAID-Clover
   

   Use

   Pcr template

   Tags

   mAID-Clover

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBD-pZ-FLAG

   Plasmid

   #183148

   Purpose

   Yeast-two-hybrid BD vector with pZ promoter (activated by ZPM)

   Depositor

   Assaf Mosquna

   Article

   Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

   Insert

   GAL4 BD
   

   Use

   Yeast two-hybrid

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSb_init containing anti SARS-CoV-2_RBD sybody Sb#16

   Plasmid

   #153524

   Purpose

   Expression of the anti SARS-CoV-2_RBD sybody Sb#16

   Depositor

   Markus Seeger

   Article

   Walter et al bioRxiv 2020.04.16.045419

   Insert

   Sb#16
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMaCTag-Z22

   Plasmid

   #120065

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with SNAP

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   SNAP
   

   Use

   Pcr template

   Tags

   SNAP

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB_TRE_Z17-PDGFR_EF1a_mCherry

   Plasmid

   #186303

   Purpose

   Plasmid for inducible mammalian cell expression of the Z17 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction.

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   Z17-PDGFR
   mCherry
   

   Use

   Tags

   HA, myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Col1a-3'probe

    Plasmid

    #20731

    Depositor

    Rudolf Jaenisch

    Article

    Beard et al Genesis. 2006 Jan . 44(1):23-8.

    Insert

    Col1a-3'probe (Col1a1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. modRNAc0-Cas9

    Plasmid

    #170180

    Purpose

    IVT template plasmid for making modified Cas9 RNA

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

    Insert

    Cas9
    

    Use

    Ivt template plasmid

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. piCRg Entry

    Plasmid

    #58904

    Purpose

    Cas9/gRNA Entry expression plasmid

    Depositor

    Danwei Huangfu

    Article

    Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

    Insert

    hSpCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJAT57

    Plasmid

    #204303

    Purpose

    Expresses Cas9 under UAS control. HDR integrant markers with DsRed expressed in flight muscles.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    MHC-DsRed
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    MHC

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMaCTag-Z34

    Plasmid

    #120062

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 3xFLAG

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    3xFLAG
    

    Use

    Pcr template

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Accessory plasmid 2

    Plasmid

    #128571

    Purpose

    for cloning identified individual SPECS

    Depositor

    Timothy Lu

    Article

    Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TagRFP

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. CL7-LbCas12aD156R

    Plasmid

    #164659

    Purpose

    Bacterial expression of mutated LbCas12aD156R using CL7/Im7

    Depositor

    Piyush Jain

    Article

    Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

    Insert

    LbCas12a-D156R
    

    Use

    Tags

    Thioredoxin, CL7, SUMO

    Expression

    Bacterial

    Mutation

    changed Aspartic Acid 156 to Arginine

    Promoter

    T7

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-V96 ELP

    Plasmid

    #67853

    Purpose

    GFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residues

    Depositor

    Andrew MacKay

    Article

    Pastuszka et al Biomacromolecules. 2012 Nov 12;13(11):34

    Insert

    GFP fused to ELP (VPGVG) repeated 96 times
    

    Use

    Tags

    GFP fused to N-terminal of ELP

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTRE:iRFP670-EFS:Cre-2A-GFP

    Plasmid

    #68477

    Purpose

    Inducible lentivirus expressing iRFP670, Cre, and GFP

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    iRFP670
    Cre-2A-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and pTRE

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMaCTag-Z26

    Plasmid

    #120069

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mAID-Clover

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mAID-Clover
    

    Use

    Pcr template

    Tags

    mAID-Clover

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMaCTag-26

    Plasmid

    #120005

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with mAID-Clover

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mAID-Clover
    

    Use

    Pcr template

    Tags

    mAID-Clover

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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