We narrowed to 16,056 results for: aga

1. pcDNA3-HA-DEPTOR S286/287/291D

   Plasmid

   #159161

   Purpose

   Expresses DEPTOR S286/287/291D mutant in mammalian cells

   Depositor

   Michele Pagano

   Article

   Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

   Insert

   DEPTOR (DEPTOR Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed serine 286,287 and 291 to aspartic acid

   Promoter

   CMV

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLCHKO_PDPR_exon_deletion_2

   Plasmid

   #155066

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLCHKO_SFRS7_exon_deletion_1

   Plasmid

   #155069

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLCHKO_MDM4_exon_deletion_1

   Plasmid

   #155073

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLCHKO_SFRS7_exon_deletion_4

   Plasmid

   #155072

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-CMV-Fyn-cGASΔN-HA

   Plasmid

   #130922

   Purpose

   Expresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the Fyn dual acylation motif (aa1-16); Puromycin selection marker

   Depositor

   Jonathan Kagan

   Article

   Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

   Insert

   Fyn-ΔNcGAS
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   cGAS N terminus (aa1-159) replaced with the dual …

   Promoter

   CMV

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-INTS3-C

   Plasmid

   #128642

   Purpose

   Express INTS3 C-termini (514-1042 aa) in E. coli with N-terminal and C-terminal His tag

   Depositor

   Yuliang Wu

   Article

   Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

   Insert

   INTS3 C-termini (INTS3 Human)
   

   Use

   Tags

   His tag on N and C-terminal

   Expression

   Bacterial

   Mutation

   INTS3 C-termini (514–1042 aa)

   Promoter

   T7

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. gh18

   Plasmid

   #106702

   Purpose

   expression of gRNA targeting GALNT18

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   GALNT18 (GALNT18 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSIREN-RetroQ-HSPE-sh1

   Plasmid

   #92033

   Purpose

   pSIREN-RetroQ vector containing shRNA sequence to HPSE

   Depositor

   Joe Landry

   Article

   Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

   Insert

   shRNA #1 to HPSE (Hpse Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 19, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. PCK2 gRNA (BRDN0001487084)

    Plasmid

    #78082

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PCK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PCK2 (PCK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. PRKACB gRNA (BRDN0001148118)

    Plasmid

    #75962

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKACB

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKACB (PRKACB Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. SLP2a-TIR-GFP

    Plasmid

    #52738

    Purpose

    Retroviral vector expresses the lipid binding domain of SLP2a fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable marker

    Depositor

    Jonathan Kagan

    Article

    Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10

    Insert

    Toll/IL-1 Receptor adaptor protein (TIRAP Human)
    

    Use

    Retroviral

    Tags

    C2A domain of Murine SLP2a and GFP

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1-85

    Promoter

    Available since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pCF821-sgCIDE-42_U6-sgRNA-EF1a-mNeonGreen

    Plasmid

    #211682

    Purpose

    U6-sgRNA-EF1a-mNeonGreen

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    SpyCas9 and sgCIDE-42 guide RNA vector
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-GenEPi

    Plasmid

    #140236

    Purpose

    Mammalian expression of Piezo1-based fluorescent force sensor GenEPi

    Depositor

    Periklis Pantazis

    Article

    Yaganoglu et al Nat Commun. 2023 Jul 19;14(1):4352. doi:

    Insert

    GenEPi (Piezo1-based fluorescent force sensor) (PIEZO1 Human)
    

    Use

    Tags

    GCaMP 6s RS1 EF4

    Expression

    Mammalian

    Mutation

    Promoter

    simian CMV IE94

    Available since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP

    Plasmid

    #64216

    Purpose

    Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptide

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA targeting ROSA26-1
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-BFP

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. R701-X65-527: His6-tev-Hs.CALM1(2-148)

    Plasmid

    #159693

    Purpose

    E. coli protein expression of His6-tev-Hs.CALM1(2-148)

    Depositor

    Dominic Esposito

    Article

    Agamasu et al Biophys J. 2019 Mar 19;116(6):1049-1063.

    Insert

    His6-tev-Hs.CALM1(2-148) (CALM1 Human)
    

    Use

    Tags

    His6-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-imp α

    Plasmid

    #119718

    Purpose

    Expresses EGFP-tagged human importinα in mammalian cells

    Depositor

    Kenji Sugimoto

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2012;76(2):38

    Insert

    importinα (KPNA2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains amino acids 251-529

    Promoter

    CMV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti- V6.3 lox-dsRED-stop-lox-eGFP-blas

    Plasmid

    #106171

    Purpose

    cre mediated red to green shift

    Depositor

    Ewa Snaar-Jagalska

    Article

    Heitzer et al Sci Rep. 2019 Mar 11;9(1):4096. doi: 10.

    Inserts

    dsRED-express2
    eGFP
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEF019 MBP-β-catenin-His

    Plasmid

    #154070

    Purpose

    Expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    CTNNB1 (CTNNB1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMG059 MBP-CREB-His

    Plasmid

    #154076

    Purpose

    Expresses MBP-CREB-His (human CREB as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    CREB1 (CREB1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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